"
Team:Tuebingen/Notebook/Protocols/onestep
From 2013.igem.org
(Difference between revisions)
| Line 21: | Line 21: | ||
<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p>Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. | + | <p>Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. Store in 1 mL aliquots at -20 °C.</p> |
</div> | </div> | ||
Revision as of 03:41, 4 October 2013
SOB Medium
Back to Protocols
Procedure
Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. Store in 1 mL aliquots at -20 °C.
