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Team:NTNU-Trondheim/Notebook/October
From 2013.igem.org
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Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below | Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below | ||
| - | <div class="col4" style="background-color:white;><a href=" | + | <div class="col4" style="background-color:white;><a href=" "> <img src=" " width="303"> |
<p style="text-align:center; color:black; "> Figure 1.</p> </div> | <p style="text-align:center; color:black; "> Figure 1.</p> </div> | ||
<div class ="row-end"> </div> | <div class ="row-end"> </div> | ||
Revision as of 08:36, 4 October 2013
Wednesday 02.10.2013
SDS-PAGE of bacterial samples with the tat_GFP_RFP (ER1) and tat_ProteinG construct
In order to determine if Protein G is produced in the S3-2B sample and wheater a GFP-RFP dimer is produced in the ER1 sample, a SDS-PAGE was performed. Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below
Figure 1.
