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BBa_K523013 Characterization
Characterisation of the iGEM plasmid BBa_K523013 we decided to use.

Sensing-Effector

Primer Resuspension
-We recieved our primers and resuspended them. Then we dilluted them in order to have a final concentration of 100umol.

Innoculating Bacteria
-From each agar plate that we inncubated over-night we innoculated one colony in order to make a glycerol stock of MMP2 and MMP9 containing bacteria the next day.

Nanoparicles

Making Nanoparticles, 2nd try
- The nanoparticles are done.
- Characterization of the new gelatin nanoparticles with the DLS : their average size is 160 nm !