Team:EPF Lausanne/Calendar/25 August 2013

From 2013.igem.org

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''Glycerol Stock'' <br>
''Glycerol Stock'' <br>
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Inoculation of competent cells trensformed with streptavidin alive gibson product to make glycerol stock.
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Inoculation of competent cells trensformed with streptavidin alive gibson product to make glycerol stock and miniprep.
<br>Planning next experiments.
<br>Planning next experiments.
<br><br>
<br><br>

Revision as of 09:34, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Glycerol Stock
Inoculation of competent cells trensformed with streptavidin alive gibson product to make glycerol stock and miniprep.
Planning next experiments.

Sensing-Effector

PCR of Cad Backbone
-We tried to amplify the Backbone for the Cad-GFP construct. But it did not work. Since the restriction digest implied that the plasmid was correct, the problem must have been with the primer temperatures. So we decided to design new primers and try another PCR with the old ones but also adding DMSO.