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Team:Heidelberg/Templates/DelH overview21
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===Results=== | ===Results=== | ||
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! Colonies !! Alignment File || Sequence | ! Colonies !! Alignment File || Sequence | ||
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| - | | I C5 || [[File: | + | | I C5 || [[File:Heidelberg_Sequencing Result pHM04 DN07 colony 05 old.clustal.txt | Sequencing of clone I C5 with DN_07]] || Amino Acid Substitution |
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| I C7 || sequencing insufficient || - | | I C7 || sequencing insufficient || - | ||
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===Vector Maps and Primers=== | ===Vector Maps and Primers=== | ||
As pointed out before, we ordered new shorter and HPLC purified primers for the assembly of pHM04 as well as for the mutagenesis. | As pointed out before, we ordered new shorter and HPLC purified primers for the assembly of pHM04 as well as for the mutagenesis. | ||
| - | [[File: | + | [[File:Heidelberg_PHM_04.png|200px|left|thumb|Vector map of the [[:File:Heidelberg_PHM04-DelH-pSB6A1(without mRFP).gb|PHM04-DelH-pSB6A1(without mRFP) Gibson plasmid]].]] |
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Latest revision as of 11:43, 4 October 2013
Results
| Colonies | Alignment File | Sequence |
|---|---|---|
| I C5 | File:Heidelberg Sequencing Result pHM04 DN07 colony 05 old.clustal.txt | Amino Acid Substitution |
| I C7 | sequencing insufficient | - |
| I C12 | sequencing insufficient | - |
Vector Maps and Primers
As pointed out before, we ordered new shorter and HPLC purified primers for the assembly of pHM04 as well as for the mutagenesis.
| Identifier | Order date | Note | Sequence |
|---|---|---|---|
| HM_20:BB_HPLC_rev | 11-09-2013 | HPLC version of HM11 Gibson-Primer rev, amplify the Backbone with overlap with the RBS and the lacI-promotor and it creates and overlap to the start of DelH | GATTTGGCGCAGGCGGCCACGGTCCATctagtatttctcctctttc |
| FS_77:BB_HPLC_rev | 11-09-2013 | Gibson-Primer rev, amplify the Backbone with overlap with the RBS and the lacI-promotor and it creates and overlap to the start of DelH | GCGATTTGGCGCAGGCGGCCACGGTCCATCTAGTATTTCTCCTCTTTC |
| HM21:fw_lacI_BbsI_Xba | 2013-09-15 | Forward primer for cutting out mutated fragment for mutagenesis | TTTTGAAGACAA CTAGGCAATACGCAA |
| HM22:rev_RBS | 2013-09-15 | Reverse Primer in RBS for mutagenesis | TTTTGAAGACAA CTCTTTCTCTAGTATGTGTGAAATTG |
| HM23:fw_RBS | 2013-09-15 | Forward Primer in RBS for mutagenesis | TTTTGAAGACAA AGAGGAGAAATACTAGATGGACCGTGGC |
| HM24:rev_BbsI_MfeI | 2013-09-15 | Reverse primer for cutting out mutated fragment for mutagenesis | TTTTGAAGACAA AATTGGACAGCGCGGCATGCCGGTTG |
