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Team:Tuebingen/Notebook/Protocols/onestep
From 2013.igem.org
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<p>Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. Store in 1 mL aliquots at -20 °C.</p> | <p>Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. Store in 1 mL aliquots at -20 °C.</p> | ||
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| + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
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Latest revision as of 12:07, 4 October 2013
ONE-STEP Buffer
Procedure
Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. Store in 1 mL aliquots at -20 °C.
Back to Protocols
