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Team:Tuebingen/Notebook/Protocols/plates
From 2013.igem.org
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<h3>Reagents</h3> | <h3>Reagents</h3> | ||
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<td style="text-align: center">1000 mL</td> | <td style="text-align: center">1000 mL</td> | ||
<td><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></td> | <td><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></td> | ||
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| + | <td>Antibiotics</td> | ||
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<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p>Add agar to LB medium and heat in microwave until all agar has completely dissolved. Cover flask with lid. <u>Perform next step at laminar flow cabinet!</u> Let cool down a bit. If required, add antibiotics to Agar-LB medium. Cast plates in petri dishes (approx. 20 mL per plate).</p> | + | <p>Add agar to LB medium and heat in microwave until all agar has completely dissolved. Cover flask with lid. <u>Perform next step at laminar flow cabinet!</u> Let cool down a bit. If required, add antibiotics to Agar-LB medium. Cast plates in petri dishes (approx. 20 mL per plate). Store plates at 4 °C.</p> |
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| + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
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Latest revision as of 12:08, 4 October 2013
LB Plates
Reagents
| 16.0 g | Agar |
| 1000 mL | LB medium |
| Antibiotics |
Procedure
Add agar to LB medium and heat in microwave until all agar has completely dissolved. Cover flask with lid. Perform next step at laminar flow cabinet! Let cool down a bit. If required, add antibiotics to Agar-LB medium. Cast plates in petri dishes (approx. 20 mL per plate). Store plates at 4 °C.
Back to Protocols
