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Team:DTU-Denmark/Notebook/11 September 2013
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{{:Team:DTU-Denmark/Templates/StartPage|11 September}} | {{:Team:DTU-Denmark/Templates/StartPage|11 September}} | ||
| - | + | Navigate to the [[Team:DTU-Denmark/Notebook/10_September_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/12_September_2013|Next]] Entry | |
=lab 208= | =lab 208= | ||
<hr/> | <hr/> | ||
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==Who was in the lab== | ==Who was in the lab== | ||
<hr/> | <hr/> | ||
| + | Henrike | ||
==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
| + | |||
| + | ===Colony PCR to verify Nir in pZA21::ara-tight=== | ||
| + | Colony PCR with sequencing primers Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq. | ||
| + | |||
| + | Re-run of yesterday's PCR with the following new annealing temperatures: pair 1: 67C, pair 2: 68C | ||
==Results== | ==Results== | ||
<hr/> | <hr/> | ||
| + | |||
| + | ===Gels=== | ||
| + | |||
| + | * 1 kb | ||
| + | * colony 1, HAO | ||
| + | * colony 2, HAO | ||
| + | * colony 3, HAO | ||
| + | * colony 4, HAO | ||
| + | * colony 5, HAO | ||
| + | * colony 6, HAO | ||
| + | * Nir colony 7, primer pair 1 | ||
| + | * Nir colony 7, primer pair 2 | ||
| + | * Nir colony 8, primer pair 1 | ||
| + | * Nir colony 8, primer pair 2 | ||
| + | * 1 kb | ||
| + | |||
| + | [[File:2013-09-11 colhao colnir seqprimer2.jpg|600px]] | ||
| + | |||
| + | Made new PCR with higher annealing temperature to obliterate unspecific binding. | ||
| + | |||
| + | * 1 kb | ||
| + | * Nir colony 1, primer pair 1 | ||
| + | * Nir colony 1, primer pair 1 | ||
| + | * Nir colony 1, primer pair 2 | ||
| + | * Nir colony 1, primer pair 2 | ||
| + | |||
| + | [[File:2013-09-11 nir col seqprimr highertemp.jpg|600px]] | ||
Navigate to the [[Team:DTU-Denmark/Notebook/10_September_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/12_September_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/10_September_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/12_September_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 12:15, 4 October 2013
11 September
Contents |
lab 208
Who was in the lab
Henrike
Procedure
Colony PCR to verify Nir in pZA21::ara-tight
Colony PCR with sequencing primers Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq.
Re-run of yesterday's PCR with the following new annealing temperatures: pair 1: 67C, pair 2: 68C
Results
Gels
- 1 kb
- colony 1, HAO
- colony 2, HAO
- colony 3, HAO
- colony 4, HAO
- colony 5, HAO
- colony 6, HAO
- Nir colony 7, primer pair 1
- Nir colony 7, primer pair 2
- Nir colony 8, primer pair 1
- Nir colony 8, primer pair 2
- 1 kb
Made new PCR with higher annealing temperature to obliterate unspecific binding.
- 1 kb
- Nir colony 1, primer pair 1
- Nir colony 1, primer pair 1
- Nir colony 1, primer pair 2
- Nir colony 1, primer pair 2
