Team:Groningen/Labwork/12 July 2013

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'''Inne'''
'''Inne'''
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<br> Made 3 times 250 ml LB Agar (20g/L LB, 16g/L)
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<br> Made 3 bottles 250 ml LB Agar (20g/L LB, 16g/L)

Revision as of 10:05, 12 July 2013

Mirjam Made a PCR with (1) no strep tag (2) strep F (3) strep R. The annealing temperature for (1) and (2) is 78 degrees Celsius. For (3) the annealing temperature is 80 degrees Celsius.

Gel purification of the strep F silk obtained yesterday.
Run a 0.8% agarose gel for 30 min at 90V.

Inne
Made 3 bottles 250 ml LB Agar (20g/L LB, 16g/L)

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