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Team:Groningen/Labwork/12 July 2013
From 2013.igem.org
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| - | <br> Made 3 bottles 250 ml LB Agar (20g/L LB, 16g/L) | + | <br> Made 3 bottles of 250 ml LB Agar (20g/L LB, 16g/L) |
Revision as of 10:05, 12 July 2013
Mirjam Made a PCR with (1) no strep tag (2) strep F (3) strep R. The annealing temperature for (1) and (2) is 78 degrees Celsius. For (3) the annealing temperature is 80 degrees Celsius.
Gel purification of the strep F silk obtained yesterday.
Run a 0.8% agarose gel for 30 min at 90V.
Inne
Made 3 bottles of 250 ml LB Agar (20g/L LB, 16g/L)
