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Team:DTU-Denmark/Methods/rehydrating primers
From 2013.igem.org
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(Created page with "{{:Team:DTU-Denmark/Templates/StartPage|Rehydrating Primers}} We dilute the lyphelized pellet in 150uL of MilliQ water (diluted 150 times) and save this as stock at -80C. From t...") |
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{{:Team:DTU-Denmark/Templates/StartPage|Rehydrating Primers}} | {{:Team:DTU-Denmark/Templates/StartPage|Rehydrating Primers}} | ||
| - | We dilute the lyphelized pellet in 150uL of MilliQ water (diluted 150 times) and save this as stock at - | + | We dilute the lyphelized pellet in 150uL of MilliQ water (diluted 150 times) and save this as stock at -80<sup>o</sup>C. From the stock we take 10uL and add to 90uL of MilliQ (diluted 10 times) to make working solution of primers kept in -20<sup>o</sup>C. |
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 18:08, 4 October 2013
Rehydrating Primers
We dilute the lyphelized pellet in 150uL of MilliQ water (diluted 150 times) and save this as stock at -80oC. From the stock we take 10uL and add to 90uL of MilliQ (diluted 10 times) to make working solution of primers kept in -20oC.
