"
Team:Groningen/Team/attributions
From 2013.igem.org
| Line 39: | Line 39: | ||
1: Brooks, Amanda E., Shane M. Stricker, Sangeeta B. Joshi, Timothy J. Kamerzell, C. Russell Middaugh, and Randolph V. Lewis. "Properties of Synthetic Spider Silk Fibers Based on Argiope Aurantia MaSp2." Biomacromolecules 9.6 (2008): 1506-510. | 1: Brooks, Amanda E., Shane M. Stricker, Sangeeta B. Joshi, Timothy J. Kamerzell, C. Russell Middaugh, and Randolph V. Lewis. "Properties of Synthetic Spider Silk Fibers Based on Argiope Aurantia MaSp2." Biomacromolecules 9.6 (2008): 1506-510. | ||
<br> | <br> | ||
| - | 2: | + | 2: Garrity, Liam F., and George W. Ordal. "Chemotaxis in< i> Bacillus subtilis</i>: How bacteria monitor environmental signals." Pharmacology & therapeutics 68.1 (1995): 87-104. |
| + | <br> | ||
</html> | </html> | ||
Revision as of 18:08, 4 October 2013
Attributions
We have thought of this project by ourselves, so it was not an assignment from one of our supervisors. The supervisors and advisers only had influence on the team as a consultant. All the design, labwork and modeling was done by the team itself and started in June. We also took care of the sponsoring and all other organisational issues, made our own presentations and poster.
Our silk we have developed was based on the article of Brooks et al. [1], but the codon optimization was done by the team itself using software written by one of the team members. We improved the backbone made by the iGEM team from Munich in 2012 and used this backbone for our constructs. We also validated that biobrick (Bba_E0840) works in bacillus subtilis (Figure 1).
The coating mechanism was developed by the team using article [2]. The knock-out chains where shipped from the university of Illinois and Buenos Aires .
|
|
Figure 1: The intensity in of ~40 cells in the GFP-channel, were analyzed from two pictures. The average intensity (AU) from the cells are plotted above with the standard deviation. A GFP from our group is shown and in a biobricked GFP (BBa_E0840) is shown in a plot. wt=wildtype, T0= before induction, T1,2,3=#h of induction with IPTG. |
References
1: Brooks, Amanda E., Shane M. Stricker, Sangeeta B. Joshi, Timothy J. Kamerzell, C. Russell Middaugh, and Randolph V. Lewis. "Properties of Synthetic Spider Silk Fibers Based on Argiope Aurantia MaSp2." Biomacromolecules 9.6 (2008): 1506-510.2: Garrity, Liam F., and George W. Ordal. "Chemotaxis in< i> Bacillus subtilis: How bacteria monitor environmental signals." Pharmacology & therapeutics 68.1 (1995): 87-104.
