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Team:Heidelberg/Templates/Del week18 overview
From 2013.igem.org
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| - | ==SDS | + | ==Validation of pFSN== |
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| + | This week we will analyze our positive D8w clone for expression of our pFSN plasmid. Therefore a SDS page will be carried out | ||
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| + | ====Results==== | ||
This week we carried out an SDS-PAGE with our clone D8w containing the pFSN plasmid. We used ''E.coli'' DH10ß as control. For our transformed ''E.coli'' a clear band at about 190 kDa was visible. This corresponds to the molecular weight of DelE (192,473 kDa). Consequently we showed that DelE is recombinantly expressed. Futhermore one could sense a very weak band at the height of about360 kDa. However this band could not be captured on an image. Consequently the samples have to be applied on a gel in a higher about again. | This week we carried out an SDS-PAGE with our clone D8w containing the pFSN plasmid. We used ''E.coli'' DH10ß as control. For our transformed ''E.coli'' a clear band at about 190 kDa was visible. This corresponds to the molecular weight of DelE (192,473 kDa). Consequently we showed that DelE is recombinantly expressed. Futhermore one could sense a very weak band at the height of about360 kDa. However this band could not be captured on an image. Consequently the samples have to be applied on a gel in a higher about again. | ||
Latest revision as of 18:28, 4 October 2013
Validation of pFSN
This week we will analyze our positive D8w clone for expression of our pFSN plasmid. Therefore a SDS page will be carried out
Results
This week we carried out an SDS-PAGE with our clone D8w containing the pFSN plasmid. We used E.coli DH10ß as control. For our transformed E.coli a clear band at about 190 kDa was visible. This corresponds to the molecular weight of DelE (192,473 kDa). Consequently we showed that DelE is recombinantly expressed. Futhermore one could sense a very weak band at the height of about360 kDa. However this band could not be captured on an image. Consequently the samples have to be applied on a gel in a higher about again.
