Revision as of 19:12, 4 October 2013

Motility assay

Ad 13 ml of the motility agar (0.4% or 0.7%) per plate.

Inoculate the plates at the center with the Strain of interest (strain A).

For every plate inoculated with Strain A, inoculate a plate with Wild type (WT).

Inoculations is done with 10 ul of liquid culture with an OD₆₀₀ of 0.4.

Grow the colonies over 16 hours.

After the 16 hour growth period the measure the spread of the colony every 2 hours.

Motility Agar 0.7% (100 ml)

compound	amount	treatment
LB broth	2 g
agar	0.7 g

Motility Agar 0.4% (100 ml)

compound	amount	treatment
LB broth	2 g
agar	0.4 g

@@ Line 27: / Line 27: @@
 <p>Inoculate the plates at the center with the Strain of interest (strain A).
 <p>For every plate inoculated with Strain A, inoculate a plate with Wild type (WT).
-<p>inoculations is done with 10 ul of liquid culture with an OD<sub>600</sub> of 0.4.
+<p>Inoculations is done with 10 ul of liquid culture with an OD<sub>600</sub> of 0.4.
 <p>Grow the colonies over 16 hours.
 <p>After the 16 hour growth period the measure the spread of the colony every 2 hours.