Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period8/Dailylog

(Difference between revisions)

Revision as of 20:30, 12 July 2013

Phage Purification May - June Notebook: May 27 - June 9 Daily Log

5/29/13

- Finished the PEG purification.

6/7/13

- Made 1 L of 10 X phage suspension buffer.

@@ Line 43: / Line 43: @@
 :[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.26_PEG_Purification|5.26 PEG Purification]]
-<br>
-<font size="4"> '''5/31/13''' </font>
-- Worked on setting up our notebook online.
-<br>
-<font size="4"> '''6/3/13''' </font>
-- Ran the CsCl purification step.
-:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]]
-- Next class we will attempt extracting the phage from the gradient.
-<br>
-<font size="4"> '''6/5/13''' </font>
-- Extracted the phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] using a syringe and a needle.
-: Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
-: Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
-: Placed these in the refrigerator.
-- Prepared stocks of bacteria for future use.
-: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]
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