Team:NTNU-Trondheim/Notebook/June

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[https://2013.igem.org/Team:NTNU-Trondheim/TransformedBricks Transformed BioBricks]
 
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Latest revision as of 20:55, 4 October 2013

Trondheim iGEM 2013

header
Mercury
Notebook

Tuesday 18.06.13



Started transforming BioBricks. Transformed some composite BioBricks consisting of constitutive promotor, RBS, a fluorescent protein and a terminator. Transformed the parts Promoter+RBS+RFP+term (BBa_I13521), Promoter+RBS+GFP+term (BBa_I13522),Promoter+RBS+CFP+term (BBa_I13600) and Pm+RBS+mCherry+term to competent ''E.coli'' DH5α cells, to have some colorful cells to show the camera crew from NRK (Norwegian broadcasting), who will visit our lab tomorrow :-) For our transformation protocol, see the protocol page.

Wednesday 19.06.13



We transformed BioBricks consisting of fluorescent proteins. Transformed the parts YFP (BBa_E0030), CFP (BBa_ I13600), RFP (BBa_E1010), GFP (BBa_E0040), BFP (BBa_K592100) and SYFP (BBa_K864100).
We transferred mCherry from agar plate to liquid medium and incubated at 37° C.

Thursday 20.06.13



We transferred the transformed cells from yesterday to liquid medium and incubated at 37°C ( protocol).
We isolated the DNA from the transformed mCherry cells by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.

Sample Concentration (ng/µl)
mCherry1 5,6
mCherry2 7,3

Friday 21.06.13



We isolated the DNA from the transformed cells by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.

Sample Concentration (ng/µl)
CFP1 8,59
CFP2 7,04
BFP 7,06
SYFP 5,42
RFP1 5,04
RFP2 7,52
YFP 6,79
GFP 5,27

Monday 24.06.13 - Friday 28.06.13



This week we have established protocols for further work and designed primers for PCR.


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