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Notebook : August 7

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003

1 - Sequensis analysis of BBa_K1155003

Abdou

The sequence analysis was good. We call our biobrick BBa_K1155003.

Objective : obtaining BBa_K1155007

1 - Ligation of RBS-LacZ-Term in pSB1C3

Anaïs, Nadia

Used quantities :

RBS-AmilCP : 2µL
Term in pSB1C3 : 2.5µL
Buffer : 1µL
Ligase : 1µL
H2O : 3.5µL

2 - Tranformation BBa_K115507 in DH5αstrain

XiaoJing

Protocol : Bacterial transformation

Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006

1 - Tranduction of Km in MG1655Z1

Nadia, XiaoJing

Protocol : Transduction

Wild type strain is MG1655Z1 and our mutant strain is BW1328: Δfnr::Km.

We did the first step of the protocol : bacteriophage stock grown on BW1328.

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@@ Line 7: / Line 7: @@
 ==='''A - Aerobic/Anaerobic regulation system'''===
-===='''Objective : obtaining Bba_K1155007'''====
+===='''Objective : obtaining BBa_K1155003'''====
-===='''1 - Ligation of RBS-LacZ-Term in PSB1C3 ====
+===='''1 - Sequensis analysis of BBa_K1155003'''====
+Abdou
+{|
+| style="border:1px solid black;padding:5px;background-color:#DE;" |
+The sequence analysis was good. We call our biobrick BBa_K1155003.
+|}
+===='''Objective : obtaining BBa_K1155007'''====
+===='''1 - Ligation of RBS-LacZ-Term in pSB1C3 ====
 Anaïs, Nadia
@@ Line 15: / Line 26: @@
 Used quantities :
 * RBS-AmilCP : 2µL
-* Term in PSB1C3 : 2.5µL
+* Term in pSB1C3 : 2.5µL
 * Buffer : 1µL
 * Ligase : 1µL
 * H2O : 3.5µL
-===='''2 - Tranformation Bba_K115507 in DH5α====
+===='''2 - Tranformation BBa_K115507 in DH5αstrain====
 XiaoJing
@@ Line 26: / Line 37: @@
 Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
-===='''Objective : characterize Bba_K1155000, Bba_K1155004, Bba_K1155005, Bba_K1155006'''====
+===='''Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006'''====
 ===='''1 - Tranduction of Km in MG1655Z1 ====
-Nadia, XioaJing
+Nadia, XiaoJing
-Protocol : [[Team:Paris_Saclay/Protocols/transduction|Transduction]]
+Protocol : [[Team:Paris_Saclay/transduction|Transduction]]
-Our Mutant bacteria was called BW : Δfnr::Km.
+Wild type strain is MG1655Z1 and our mutant strain is BW1328: Δfnr::Km.
-Our wild type bacteria was called MG1655Z1.
-We did the first step of the protocol : bacteriophage stock which packed Km gene.
+We did the first step of the protocol : bacteriophage stock grown on BW1328.
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Team:Paris Saclay/Notebook/August/7

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