Team:EPF Lausanne/Calendar/22 August 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display </font> <BR>
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Sensing <BR>
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Transformation didn't work so it was repeated.
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<br><br>
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'''Gradient PCR of Sensing Backbones''' <BR>
 
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We did another gradient PCR for the backbones but for the constructs three sensing constructs in order to check if the problem in the previous pcr were the primers or actually the plasmid. Again it did not work.
 
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<font size = "4"> Sensing-Effector </font> <BR>
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'''Nanoparticles'''
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''Gradient PCR of Sensing Backbones'' <BR>
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<BR>- Affinity purification of biotinylated nanoparticles with Streptavidin Magnetic Particles. Measurement of the products with DLS.
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-We did another gradient PCR for the backbones but for the three sensing constructs in order to check if the problem in the previous pcr were the primers or actually the plasmid. Again, it did not work.
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<BR>- ELISA-like assay in order to detect the presence of biotin.  
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<BR> <BR>
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<font size = "4"> Nanoparticles</font> <BR>
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''Making Nanoparticles, 2nd try'' <BR>
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-Affinity purification of biotinylated nanoparticles with Streptavidin Magnetic Particles. Measurement of the products with DLS.
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<BR>-ELISA-like assay in order to detect the presence of biotin.  
{{Template:EPFL2013Footer}}
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Latest revision as of 21:57, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Transformation didn't work so it was repeated.




Sensing-Effector

Gradient PCR of Sensing Backbones
-We did another gradient PCR for the backbones but for the three sensing constructs in order to check if the problem in the previous pcr were the primers or actually the plasmid. Again, it did not work.

Nanoparticles

Making Nanoparticles, 2nd try
-Affinity purification of biotinylated nanoparticles with Streptavidin Magnetic Particles. Measurement of the products with DLS.
-ELISA-like assay in order to detect the presence of biotin.

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/22_August_2013"