Team:Paris Bettencourt/Notebook/Drug Screening/Tuesday 20th August.html

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Plasmid mini-prep of transformations
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Digestion of FprA with NdeI and AvrII
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<br>Nano dropped
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<ol><li>pETDuet: 59.1ng/ul</li>
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    <li>pETDuet: 45.9ng/ul</li>
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    <li>pJET Fdx: 54.2ng/ul</li>
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    <li>pLS FRP: 19.7ng/ul</li></ol>
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<br>Digestion of FprA with NdeI and AvrII
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<br>Gel Clean up of FprA fragment
<br>Gel Clean up of FprA fragment
<br>Digestion of pETDuet-1 with NdeI and AvrII
<br>Digestion of pETDuet-1 with NdeI and AvrII

Latest revision as of 22:08, 4 October 2013

Target

Tuesday 20th August

Cloning of FdxA and FprA

Digestion of FprA with NdeI and AvrII
Gel Clean up of FprA fragment
Digestion of pETDuet-1 with NdeI and AvrII
Digestion of FdxA with NcoI and HindIII
Digestion of pETDuet-1 NcoI and HindIII

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