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Team:Paris Bettencourt/Notebook/Drug Screening/Thursday 22nd August.html
From 2013.igem.org
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<li>pLS FprA with FprA F/R</li> | <li>pLS FprA with FprA F/R</li> | ||
<li>pET duet with MCSI F/R</li> | <li>pET duet with MCSI F/R</li> | ||
| - | <li>pACYC duet with MCSI F/R</li> | + | <li>pACYC duet with MCSI F/R</li></ul> |
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</div> | </div> | ||
</html> | </html> | ||
Latest revision as of 22:41, 4 October 2013
Target
ASDFThursday 22nd August
Nano drop
Plasmid mini-prep of transformationsNano dropped
- pETDuet: 59.1ng/ul
- pETDuet: 45.9ng/ul
- pJET Fdx: 54.2ng/ul
- pLS FRP: 19.7ng/ul
Digest pLS FrpA with NdeI and AvrII
Digest pETDuet with NdeI and AvrII
Digest FdxA gBlock with NcoI and HindIII
Digest pETDuet with NcoI and HindIII
Ligation of FprA with pETDuet
Ligation of FdxA with pETDuet
Transformation of pET FprA and pET FdxA into NEBTurbo
PCR of
- FdxA gBlock with FdxA F/R
- pLS FprA with FprA F/R
- pET duet with MCSI F/R
- pACYC duet with MCSI F/R
