"
Team:NTNU-Trondheim/Notebook/October
From 2013.igem.org
| (One intermediate revision not shown) | |||
| Line 19: | Line 19: | ||
| - | <div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/ | + | <div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/2/21/Logo2_NTNU.png" alt="header" border="0" usemap="#igemmap" width="1000" height="400"></center></div> |
| Line 122: | Line 122: | ||
<div class ="row-end"> </div> | <div class ="row-end"> </div> | ||
</div> | </div> | ||
| + | |||
| + | <div class="row"> | ||
| + | <div class="col12-3" align = "justify"> | ||
| + | <div class="col12-3" align = "center" style="background-color:white;> | ||
| + | <p style="text-align:center; color:black; "> Wednesday 02 - Friday 04.10.2013</p> </div> | ||
| + | <div class ="row-end"> </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="row"> | ||
| + | <div class="col12-3" align = "justify"> | ||
| + | <p> | ||
| + | As the first attempt to send in the tat_GFP_RFP construct to iGEM Headquarters failed, we redid the cloning as we did 26-28.09.2013 and sent in a new construct. | ||
</div> | </div> | ||
Latest revision as of 22:43, 4 October 2013
In order to determine if Protein G is produced in the S3-2B sample and wheater a GFP-RFP dimer is produced in the ER1 sample, a SDS-PAGE was performed. Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below
Figure 1: SDS-PAGE of bacterial samples. From the left: wild type ER2566, tat_GFP_RFP and tat_ProteinG
A new test on the Pm/XylS promoter was performed with the same conditions as earlier.
As the first attempt to send in the tat_GFP_RFP construct to iGEM Headquarters failed, we redid the cloning as we did 26-28.09.2013 and sent in a new construct.
