Template:Team:Uppsala/JS/notebook

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MikaelS (Talk | contribs)

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Latest revision as of 23:26, 4 October 2013 (view source)

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<html>

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var month = ~~new Date().getMonth()~~;

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var month = 8;

var year = new Date().getFullYear();

var cal_table = '';

var eventString;

var days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);

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var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385'

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var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385','t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399','t2013910','t2013911','t2013912','t2013917','t2013918','t2013919','t2013920','t2013921','t2013924','t2013927','t2013928','t2013101','t2013102','t2013104','t20131011','t20131012','t20131013');

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,'t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399'

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var inputs = 106;

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,'t2013910','t2013911','t2013912','~~t2013913~~','~~t2013914~~','~~t2013915~~','~~t2013919~~');

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var inputs = 96;

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Date.prototype.getWeek = function()

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eventString = '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';

document.getElementById('file_holder').innerHTML = eventString;

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document.getElementById('calendar').style.marginBottom="350px";

}

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eventString = eventString + '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';

}

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document.getElementById('calendar').style.marginBottom="350px";

document.getElementById('file_holder').innerHTML = eventString;

eventString = '';

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ds = '<div id="dairy-text"><h1>Wednesday 2013-08-14</h1> Name of participants: Christoffer A, Stephanie H, Alona N, Mikael S, Viktor B, Viktor T, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Peter C, Lovisa P <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 115. pSB3K3-J23101-B0034-His-TAL E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <h2>Todays work:</h2> Re-streak: 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 pSB1K3-J23110-B0034-aeBlue (should be subcloned to pSB1C3 after) pSB3K3-J23110-B0034-AmilCP (should be subcloned to pSB1C3 after) pSB3K3-J23110-B0034-asCP (should be subcloned to pSB1C3 after) Re streak 15/8 or 16/8 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink O/N: 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP Streak from frozen stock: 111. pSBLb4C15-red Gradient touchdown colony PCR: CSP Gel Electrophoresis: CBP (colony PCR from Lb.Reuteri) CSP (colony PCR from Lb.Reuteri) Mcherry (from plasmid preparation) 256. pSBLb4C15-pem PCR purification: M1 M2 M3 M4 CB1 CB2 256. pSBLb4C15-pem (P1) Digestion: M3 (x+p) CB1 (x+p) P1 (x+p) 111.7 pSB4C15-red (e+p) Bleomycin resistance cassette (SacI) pEL PCR product with e. coli ori (ClaI) Lb3 reuteri DSM 20016 pVS2 (ClaI) CP promoters with amilGFP, aeBlue,tsPurple Ligation: M3 with pSB1C3, pSBLB4C15 + CP8, CP1 CB1 with pSB1C3 P1 with pSB1C3 pVS2 origin of replication with pEL PCR-product, cut by ClaI CP promoters with amilGFP, aeBlue,tsPurple Frozen stock and plasmid preparation: 275.1 pSBLb4C15-red (dam-)* 275.2 pSBLb4C15-red (dam-) 276. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-) 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-) *O/N of clone 1 was made from a very small colony, its colour wasn’t as strong as clone 2’s after growing overnight. Transformation: 109.pEL3S17-K206000-B0034-IspA x 110.pEL3S17-K206001-B0034-IspA x 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His x 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His x 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His x 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His x 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His x 184.pSB1C3-B0034-CrtZ x 208.pSB1A3-B0034-Thioredoxin-ZCD-His x 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His x 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His x 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His x 267.psB3K3-K206000-B0034-IspA x 268.psB3K3-K206001-B0034-IspA x 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His x 272 pSB1K3-B0034-Thioredoxin-ZCD-His x 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His x Plasmid prepp: 280. pSB1A3-B0034-aeBlue (should be subcloned to pSB1C3 after, give Erik the subclones version) <h2>Results:</h2> Gel Electrophoresis: CBP (colony PCR from Lb.Reuteri) CB1: 54.3o C (NC ok!), CB2: 63.3o C (NC ok!) → Correct bands!! CSP (colony PCR from Lb.Reuteri) CS1: 55.7o C (NC ok!), CS2: 65.6o C (NC ok!) → No bands!! (not even primer dimer) Mcherry (from plasmid preparation) M1: 45.0o C (NC ok!), M2: 47.9o C (NC ok!), M3: 50.5o C (NC ok!), M4: 54.3o C (NC ok!) → Correct bands!! 256. pSBLb4C15-pem: P1: 47.9o C (NC ok!) → Correct bands!! PCR purification: M1: 65.9 ng/µl → Store in freezer! M2: 200.6 ng/µl → Store in freezer! M3: 136.8 ng/µl → For digest! → Store in freezer! M4: 49.5 ng/µl → Store in freezer! CB1: 90.8 ng/µl → For digest! → Store in freezer! CB2: 56.7 ng/µl → Store in freezer! P1: 56.2 ng/µl → For digest! → Store in freezer! Plasmid preparation: 275.1 PK401 dam- pSBLb4C15-red (dam-): 13.5 ng/µl 275.2 PK401 dam- pSBLb4C15-red (dam-): 27.9 ng/µl 276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-): 18.3 ng/µl 277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-): 26.6 ng/µl Western blot: Transfer overnight not complete but continued with anyway Very promising results, bands can be seen around 50 kDa but the ladder is pretty weak so we can’t be absolutely sure. Looks like the J23101 promotor does’nt work. <h2>Characterization experiments</h2> Characterization of Resveratrol production through spectroscopy and HPLC Blotting and development of constructs: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 115. pSB3K3-J23101-B0034-His-TAL <h2>Other experiments:</h2> Making MRS-broth (2x600 ml) New buffers for western blot made </div>'

}

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else if(id == 'd2013815')

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{

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ds = '<div id="dairy-text"><h1>Thursday 2013-08-15</h1> Name of participants: Christoffer Ahlström, Stephanie Herman, Alona Nyberg, Mikael Strandgren, Alexander, Malin, Pontus, Niclas, Christoffer, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H., Peter C, Lovisa P, Theodor L <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Digestions: Erythromycin resistance cassette from pLUL631 (SacI, Tango buffer) Bleomycin resistance cassette (SalI, FD buffer) O/N Screening glycerolstock: 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His Re-streak: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 184.pSB1C3-B0034-CrtZ 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 110.pEL3S17-K206001-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His Plasmid prep: 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP Transformation: 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 292. PK401 dam- pSBLb4C15-CP1-mCherry (dam-) 294. PK401 dam- pSBLb4C15-CP8-mCherry (dam-) Ligations: pSBLB4C15 and Bleomycin resistance cassette cut with SacI and SalI Spreading of strains: 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry E. coli PK401 dam-: 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry Screening PCR and gel electrophoresis <h2Results:</h2> Yesterdays buffers for western blot resulted in smear. <h2>Characterization experiments</h2> Characterization of Resveratrol production through spectroscopy and HPLC <h2>Other experiments</h2> Making MRS-agar plates: 47 Chloramphenicol 47 Without antibiotics Testing yesterdays buffers for western blot. New separation- and stacking buffers for western blot with a factor 1000 concentration of Tris.</div>'

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}

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else if(id == 'd2013816')

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{

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ds = '<div id="dairy-text"><h1>Friday 2013-08-16</h1> Name of participants: Anton Berglund, Stephanie Herman, Mikael Strandgren, Alexander, Pontus, Nils, Christoffer, Sabri, Hampus.E, Kristoffer L, Victor S, Marcus H, Karl H, Theodor L, Peter C <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> O/N Screening & glycerolstock: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 184.pSB1C3-B0034-CrtZ 208. pSB1A3-B0034-Thioredoxin-ZCD-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 2 74.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His Streak from frozen stock: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red Digestion Erythromycin cassette amplicon from pLUL631 (SalI) Ligation pSB1C3-pemp 298. pSBLb4E15-red (E-cassette amplicon from pLUL631 with pSBLb4C15 (SacI + SalI)) 297. pSBLb4B15-red (Bleomycin cassette with pSBLb4C15 (SacI + SalI)) Plasmid preparation: 204.1 pEL3S17-J23110-B0034-HIS-STS 204.3 pEL3S17-J23110-B0034-HIS-STS 204.4 pEL3S17-J23110-B0034-HIS-STS 254.1 pSB1C3-gus 254.2 pSB1C3-gus 255.1 pSBLB4C15-pemp 255.2 pSBLB4C15-pemp 255.3 pSBLB4C15-pemp 255.4 pSBLB4C15-pemp O/N: 204.1 pEL3S17-J23110-B0034-HIS-STS 204.3 pEL3S17-J23110-B0034-HIS-STS 204.4 pEL3S17-J23110-B0034-HIS-STS 254.1 pSB1C3-gus 254.2 pSB1C3-gus 255.1 pSBLB4C15-pemp 255.2 pSBLB4C15-pemp 255.3 pSBLB4C15-pemp 255.4 pSBLB4C15-pemp 257.1 pSB1C3-pemp 257.2 pSB1C3-pemp 257.3 pSB1C3-pemp 280. pSB1K3-J23110-B0034-aeBlue 281. pSB3K3-J23110-B0034-amilCP 282. pSB3K3-J23110-B0034-asCP Transformation: 297. PK401 dam- pSBLb4B15-red 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 155pSB1C3-j23110-RBS-meffRed 156pSB1C3-j23106-RBS-meffRed 157pSB1C3-j23110-RBS-spisPink 158pSB1C3-j23106-RBS-spisPink 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208.pSB1A3-B0034-Thioredoxin-ZCD-His 247pSB1C3-J23110-RBS-tsPurple 248pSB1C3-J23106-RBS-tsPurple 249pSB1C3-J23110-RBS-scOrange 250pSB1C3-J23106-RBS-scOrange 251pSB1C3-J23110-RBS-fwYellow 252pSB1C3-J23106-RBS-fwYellow 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272 pSB1K3-B0034-Thioredoxin-ZCD-His 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS <h2>Results:</h2> Plasmid prep: 204.1 pEL3S17-J23110-B0034-HIS-STS: 9.7 ng/µl 204.3 pEL3S17-J23110-B0034-HIS-STS: 11.9 ng/µl 204.4 pEL3S17-J23110-B0034-HIS-STS: 7.6 ng/µl 254.1 pSB1C3-gus: 6.7 ng/µl 254.2 pSB1C3-gus: 11.5 ng/µl 255.1 pSBLB4C15-pemp: 9.9 ng/µl 255.2 pSBLB4C15-pemp: 6.3 ng/µl 255.3 pSBLB4C15-pemp: 5.0 ng/µl 255.4 pSBLB4C15-pemp: 4.6 ng/µl Since the concentrations were so low a new set of overnight cultures were prepared. Spectophotometry: Inconclusive results. <h2>Characterization experiments</h2> Characterization of Resveratrol production through spectroscopy and HPLC Testing of new buffers for western blot. <h2>Other experiments</h2> Making MRS-broth (2x600 ml) Making LB-agar plates: 173 Chloramphenicol (chloramphenicol was forgotten in 26 of the plates, but they had already been mixed with 25 others, so also these had to be discarded. 5 plates of doubtful antibiotic content had already been used by the carotenoid group, they were probably ones with ab. E. colis containing pSB3K3 were spread on some of the mixed plates.) 28 Kanamycin</div>'

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}

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else if(id == 'd2013817')

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{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-17</h1> Name of participants: Anders Edlund, Alexander, Pontus, Sabri, Hampus.E, Kristoffer L, Viktor @, Marcus H. Ken B-A, Karl H, Theodor L <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Restreak 254.3 pSB1C3-Gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry 297. pSBLb4B15-red 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS Plasmid preparation: 254.1 pSB1C3-Gus 254.2 pSB1C3-Gus 255.1 pSBLB4C15-pemp 255.2 pSBLB4C15-pemp 257.1 pSB1C3-pemp 257.2 pSB1C3-pemp 184.pSB1C3-B0034-CrtZ 280. pSB1K3-J23110-B0034-aeBlue 281. pSB3K3-J23110-B0034-amilCP 282. pSB3K3-J23110-B0034-asCP Digest: 147.pSB1C3-B0034-Thioredoxin (X,P) Ligation: 147.pSB1C3-B0034-Thioredoxin, 91.BBa_J61002 (amp)-J23106, 36.pEL3S17-red ---> 300.pEL3S17-J23106-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin, 92.BBa_J61002 (amp)-J23110, 36.pEL3S17- red ---> 301.pEL3S17-J23110-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin, 103.pSB1K3-J23101, 36.pEL3S17-red ---> 299.pEL3S17-J23101-B0034-Thioredoxin Transformation: 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin <h2>Results:</h2> Plasmid preparation: 254.1 pSB1C3-Gus 110 ng/uL 254.2 pSB1C3-Gus 132 ng/uL 255.1 pSBLB4C15-pemp156 ng/uL 255.2 pSBLB4C15-pemp 158 ng/uL 257.1 pSB1C3-pemp 146 ng/uL 257.2 pSB1C3-pemp 140 ng/uL</div>'

+

}

+

else if(id == 'd2013818')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-18</h1> Name of participants: Anders Edlund, Alexander, Pontus, Kristoffer L, Marcus H. <h2>Ongoing constructs:</h2> <h2>E. coli:</h2> 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> ON: 134. pSB1C3-B0034-CBP 254. pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry pSBLB4C15-red pSBLB4C15-red dam- Restreak: 68.1 pSB3K3-B0034-BFP 68.2 pSB3K3-B0034-BFP 68.4 pSB3K3-B0034-BFP 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.pSB1C3-B0034-Thioredoxin 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.pSB3K3-K206000-B0034-IspA 268.pSB3K3-K206001-B0034-IspA 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.pEL3S17-J23106-B0034-Thioredoxin PCR: 134. pSB1C3-B0034-CBP 254. pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Digest: 125. pSB1C3-ZCD-His 127. pSB1A3-B0034-UGTCs2-His 129.pSB1C3-Idi(mut) 184.pSB1C3-B0034-CrtZ Ligation: 4.pSB1A3-red 7.pSB3K3-red 36.pEL3S17-red 91.j23106 92.j23110 103.pSB1K3-j23101 125. pSB1C3-ZCD-His 127. pSB1A3-B0034-UGTCs2-His 129.pSB1C3-Idi(mut) 184.pSB1C3-B0034-CrtZ Plasmidprep: 184.pSB1C3-B0034-CrtZ Transformation: 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 302. idi (mut)-B0034-Ispa 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ 305.pSB1A3-j23110-B0034-CrtZ O/N screening: 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 <h2>Results:</h2> Failed: 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208.pSB1A3-B0034-Thioredoxin-ZCD-His 272 pSB1K3-B0034-Thioredoxin-ZCD-His 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His</div>'

+

}

+

else if(id == 'd2013819')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-19</h1> Name of participants: Anton Berglund, Viktor Blomkvist, Mikael Strandgren, Stephanie Herman, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alexander, Pontus, Sabri <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Screening 134.1 pSB1C3-CBP 254 pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Screening O/N: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.B0034-Thioredoxin 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.J23106-B0034-Thioredoxin 202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Digestion & screening: 254.1 pSB1C3-gus: XbaI & PstI 254.2 pSB1C3-gus: XbaI & PstI 255.1 pSBLB4C15-pemp: EcoRI & PstI 255.2 pSBLB4C15-pemp: EcoRI & PstI 257.1 pSB1C3-pemp: EcoRI & PstI 257.2 pSB1C3-pemp: EcoRI & PstI Frozen stock: 134.1 pSB1C3-CBP 275. pSBLB4C15-red (dam-) 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry 1 ml overnight culture: 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 139. pSB3K3-J23101-B0034-BFP Plasmid preparation 111.7(1) pSBLB4C15-red 111.7(2) pSBLB4C15-red 134.1 pSB1C3-CBP 275 pSBLB4C15-red (dam-) 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Overnight culture: Lb11.2 plantarum 36E-no plasmid Phusion PCR: usp45 (synthesized signal peptide from Lactococcus lactis) Restreak 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ digest 36. pEL3S17-red Ligation 108.pEL3S17-J23106-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-His 141.pEL3S17-J23101-B0034-UGTCs2-His 142.pEL3S17-CP1-B0034-UGTCs2-His 143.pEL3S17-CP8-B0034-UGTCs2-His Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Two step Assembly: 92. BBa_J61002-J23110, 149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed 91. BBa_J61002-J23106, 149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed 92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink 91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink 92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple 91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple <h2>Results:</h2> Screening 134.1 pSB1C3-CBP: showed the correct band! 254 pSB1C3-gus: showed a very weak band, not sure if correct! 288.1 pSB1C3-pem: showed the correct band! 288.2 pSB1C3-pem: showed the correct band! 288.3 pSB1C3-pem: did not show the correct band! 288.4 pSB1C3-pem: showed the correct band! 289.1 pSB1C3-mCherry: showed the correct band! 289.2 pSB1C3-mCherry: did not show the correct band! 289.3 pSB1C3-mCherry: showed the correct band! 289.4 pSB1C3-mCherry: showed the correct band. 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His: fail 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His: fail 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His: fail 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His: fail 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: fail 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: fail 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: fail Digestion & screening: 254.1 pSB1C3-gus: XbaI & PstI, did not show the correct bands! 254.2 pSB1C3-gus: XbaI & PstI, did not show the correct bands! 255.1 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands! 255.2 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands! 257.1 pSB1C3-pemp: EcoRI & PstI, showed the correct bands! 257.2 pSB1C3-pemp: EcoRI & PstI, showed the correct bands! Plasmid preparation 111.7(1) pSBLB4C15-red: 81.7 ng/µl 111.7(2) pSBLB4C15-red: 93.9 ng/µl 134.1 pSB1C3-CBP: 163.8 ng/µl 275 pSBLB4C15-red (dam-): 92.4 ng/µl 288.1 pSB1C3-pem: 211.2 ng/µl 288.2 pSB1C3-pem: 193.2 ng/µl 288.4 pSB1C3-pem: 193.9 ng/µl 289.1 pSB1C3-mCherry: 230.4 ng/µl 289.3 pSB1C3-mCherry: 229.7 ng/µl 289.4 pSB1C3-mCherry: 239.7 ng/µl</div>'

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}

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else if(id == 'd2013820')

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{

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ds = '<div id="dairy-text"><h1>Tuesday 2013-08-20</h1> Name of participants: Anton Berglund, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Theodor L, Emil M, Victor S <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His (201. E.Coli. Nissle - CrtY-CrtI) 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Characterisation of the CP promoters with facs: 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP Digestion: USP. with E, P 191. pSB1A2-fusionGFP with X, P 280. pSB1A3-B0034-aeBlue with X, P 147.B0034-Thioredoxin (E, S) 147.B0034-Thioredoxin (X,P) 27.pEL3K16-red (E,P) 125.pSB1C3-ZCD-His (X,P) Ligation: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin Transformation: 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ Screen/ ON: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Plasmid prep. / Seq. prep. 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ Gel: 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Phusion PCR: ->219.pSB1C3-Miraculin Making competent Lactobacillus reuteri Lb12.1 reuteri DSM 20016 plasmid free: eight Lb12.2 reuteri DSM 20016 plasmid free: eight Electroporation Lb3.2 reuteri DSM 20016 pVS2: two 111.7(1) pSBLB4C15-red: one 111.7(2) pSBLB4C15-red: two 255.1 pSBLB4C15-pemp: one 255.2 pSBLB4C15-pemp: one 275 pSBLB4C15-red (dam-): three <h2>Results:</h2> Electroporation Lb3.2 reuteri DSM 20016 pVS2: one of the two survived 111.7(1) pSBLB4C15-red: survived 111.7(2) pSBLB4C15-red: one of the two survived 255.1 pSBLB4C15-pemp: survived 255.2 pSBLB4C15-pemp: survived 275 pSBLB4C15-red (dam-): two of the three survived</div>'

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}

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else if(id == 'd2013821')

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{

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ds = '<div id="dairy-text"><h1>Wednesday 2013-08-21</h1> Name of participants: Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Alexander, Pontus, Sabri, Malin, Magnus, christoffer, Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E, Lovisa P <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 204(1-4). pEL3S17-J23110-B0034-His-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Digestion: usp45: XbaI & AgeI 191.1 pSB1A2-fusionGFP: XbaI, PstI 191.1 pSB1A2-fusionGFP: NgoMIV & PstI 275. pSBLB4C15-red (dam-): EcoRI & PstI 289.1 pSB1C3-mCherry: NgoMIV & PstI 289.1 pSB1C3-mCherry: XbaI, PstI 297.1 pSBLB4B15-red (dam-): EcoRI & PstI Ligation: pSBLB4B15-CP11-fusionGFP (297.1+63.2+191.1) pSBLB4B15-CP30-fusionGFP (297.1+65.1+191.1) pSBLB4B15-CP11-usp45-fusionGFP (297.1+63.2+usp45+191.1) pSBLB4B15-CP30-usp45-fusionGFP (297.1+65.2+usp45+191.1) pSBLB4B15-CP11-usp45-mCherry (297.1+63.2+usp45+289.1) pSBLB4B15-CP30-usp45-mCherry (297.1+65.2+usp45+289.1) pSB1C3-usp45 (1+usp45) pSB1C3-usp45 (6+usp45) pSBLb4C15-CP1-B0034-BFP pSBLb4C15-CP8-B0034-BFP pSBLb4C15-CP11-B0034-BFP pSBLb4C15-CP29-B0034-BFP pSBLb4C15-CP30-B0034-BFP pSBLb4C15-CP41-B0034-BFP pSBLb4C15-CP44-B0034-BFP pSBLb4C15-CP11-aeblue pSBLb4C15-CP30-aeblue pSBLb4B15-CP11-mcherry pSBLb4B15-CP30-mcherry pSBLb4B15-CP11-tspurple pSBLb4B15-CP30-tspurple pSBLb4B15-CP11-amilCP pSBLb4B15-CP30-amilCP pSBLb4B15-CP11-amilGFP pSBLb4B15-CP30-amilGFP 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Transformation: 108.pEL3S17-J23106-B0034-IspA 141.pRL3S17-J23101-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 272.pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ 204(1-4) Re-streak: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Screen/ ON: 219.pSB1C3-Miraculin 304.pSB1A3-J23106-B0034-CrtZ 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 280. pSB1A3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP Plasmid prep. 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His Mutagenesis PCR: 336. pSB1C3-spisPink 337. pSB1C3-scOrange 338. pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 257. pSB1C3-pemp 288. pSB1C3-pem <h2>Other experiments:</h2> Phosphorylation of primers (20µl of each): Pem-mutE_F Pem-mutE_R Pem-mutS_F Pem-mutS_R Cleaning of electroporation cuvettes</div>'

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}

+

else if(id == 'd2013822')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-22</h1> Name of participants: Anders Edlund, Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His (201. E.Coli. Nissle - CrtY-CrtI) 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Competent cell preparation: Lb11.1 plantarum 36E no plasmid Lb12.1 reuteri DSM 20016 no plasmid Lb12.2 reuteri DSM 20016 no plasmid Digest: 147.B0034-Thioredoxin (E, S) 147.B0034-Thioredoxin (X,P) 27.pEL3K16-red (E,P) 125.pSB1C3-ZCD-His (X,P) Ligation: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin Transformation: 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP <strike>3.2. pVS2 (died in electroporation) 310. pSBLB4B15-CP11-fusionGFP (died in electroporation) 318. pSBLB4B15-CP11-mCherry (died in electroporation) 320. pSBLB4B15-CP11-amilGFP (died in electroporation) 313. pSBLB4B15-CP30-usp45-fusionGFP (died in electroporation) 317. pSBLB4B15-CP30-tsPurple (died in electroporation) 331. pSBLB4C15-CP41-BFP (died in electroporation) 332. pSBLB4C15-CP44-BFP (died in electroporation)</strike> + other E. coli transformations Mutagenesis PCR: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R Screen/ ON: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Plasmid prep. / Seq. prep. 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ Gel: 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Phusion PCR: ->219.pSB1C3-Miraculin <h2>Results:</h2> Mutagenesis PCR: 257. pSB1C3-pemp: Problem with PCR-machine, paused at 98*C for 25 min. → re-do. 288. pSB1C3-pem: --”-- Transformation: 11.1 Lb. plantarum 36E: 3.2 pVS2: survived electroporation 3.2 pVS2: died during electroporation 310. pSBLB4B15-CP11-fusionGFP: died during electroporation 311. pSBLB4B15-CP30-fusionGFP: survived electroporation 312. pSBLB4B15-CP11-usp45-fusionGFP: survived electroporation 313. pSBLB4B15-CP30-usp45-fusionGFP: died during electroporation 316. pSBLB4B15-CP11-tsPurple: survived electroporation 317. pSBLB4B15-CP30-tsPurple: died during electroporation 12.2 Lb. reuteri DSM 20016: 3.2 pVS2: survived electroporation 318. pSBLB4B15-CP11-mCherry: died during electroporation 319. pSBLB4B15-CP30-mCherry: survived electroporation 320. pSBLB4B15-CP11-amilGFP: died during electroporation 321. pSBLB4B15-CP30-amilGFP: survived electroporation 322. pSBLB4B15-CP11-amilCP: survived electroporation 323. pSBLB4B15-CP30-amilCP: survived electroporation 324. pSBLB4C15-CP11-aeBlue: survived electroporation 12.1 Lb. reuteri DSM 20016: 325. pSBLB4C15-CP30-aeBlue: survived electroporation 326. pSBLB4C15-CP1-BFP: survived electroporation 327. pSBLB4C15-CP8-BFP: survived electroporation 328. pSBLB4C15-CP11-BFP: survived electroporation 329. pSBLB4C15-CP29-BFP: survived electroporation 330. pSBLB4C15-CP30-BFP: survived electroporation 331. pSBLB4C15-CP41-BFP: died during electroporation 332. pSBLB4C15-CP44-BFP: died during electroporation <h2>Characterization:</h2> Western blot analysis (SDS-page) on constructs: 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) (neg.contr) <h2>Other experiments:</h2> Phosphorylation of primers (60 µl of each): Pem-mutE_F Pem-mutE_R Pem-mutS_F Pem-mutS_R</div>'

+

}

+

else if(id == 'd2013823')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-23</h1> Name of participants: Anton Berglund, Stephanie Herman, Viktor Berglund, Mikael Strandgren, Anders Edlund, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M <h2>Ongoing constructs:</h2> E. coli (D5α): 111.7 pSBLb4C15-red 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Todays work:</h2> Gel electrophoresis: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R 111.7 pSBLb4C15-red (cut with E and P) (to get reference/examine cutsites) Mutagenesis PCR: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R Touchdown with Tm1+2 and Tm1+4. Transformation: 102.pSB1C3-CrtE 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208. pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 300. pEL3S17-J23106-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin Re-streak: 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Screening O/N: 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 222.pSB3K3-CP1-B0034-UGTCs2-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin O/N: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Glycerol stock Plasmidprep/sekvensprep: 108.pEL3S17-J23106-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 299.pEL3S17-J23101-B0034-Thioredoxin <h2>Results:</h2> Gel electrophoresis: 257. pSB1C3-pemp with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!* 288. pSB1C3-pem with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!* 111.7 pSBLb4C15-red (cut with E and P): No band *→ redo mutagenesis PCR with higher temperatures</div>'

+

}

+

else if(id == 'd2013824')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-24</h1> Name of participants: Mikael Strandgren, Anders Berglund, Alexander, Pontus, Sabri, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M <h2>Ongoing constructs:</h2> Escherichia coli 257. pSB1C3-pemp 288. pSB1C3-pem 136. pSB1C3-B0034-USP pSB1C3-B0034-usp45 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS Lactobacillus reuteri: Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP Lactobacillus plantarum: Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple <h2>Todays work:</h2> Mutagenesis 338.pSB1C3-meffBlue Digest: 200.pSB1C3-B0034-asCP E,X 148.pSB1C3-RBS-gfasPurple E,X 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 E,X 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI) 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI) Ligation: 200.pSB1C3-B0034-asCP+91.BBa_J61002 (amp)-J23106→ → 343.J23106-B0034-asCP 148.pSB1C3-RBS-gfasPurple+91.BBa_J61002 (amp)-J23106→ → 160.j23106-RBS-gfasPurple 148.pSB1C3-RBS-gfasPurple+92.BBa_J61002 (amp)-J23110→ → 159j23110-RBS-gfasPurple 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1+6.pSB1K3-red→ →215.pSB1K3-pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→ → 303.pSB1K3-J23101-B0034-CrtZ 127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→ → 223.pSB3K3-CP8-B0034-UGTCs2-His Transformation: 223.pSB3K3-CP8-B0034-UGTCs2-His 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 343.pSB1C3-J23106-B0034-asCP 160.pSB1C3-j23106-RBS-gfasPurple 159.pSB1C3-j23110-RBS-gfasPurple 303.pSB1K3-j23101-B0034-CrtZ 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 336.pSB1C3.spisPink 337.pSB1C3.scOrange 284.pSB1C3.B0034-aeBlue 285.pSB1C3.J23110-B0034-aeBlue 286.pSB1C3.J23110-B0034-amilCP 287.pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 284. pSB1C3-B0034-aeBlue 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP O/N screening: 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP 136. 312 313 315 321 325 327 332 317 O/N: 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin Plasmid prepp & sequence prepp 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Gel electrophoresis: Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R + E. coli transformations PCR-purification: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R DpnI: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R <h2>Results:</h2> Transformations: All transformations spread on Bleomycin/zeocin have grown! If they are the right lactobacilli with the right plasmid remains to be seen, there seem to be a lot of contamination in the form of “clouds” covering all plates. Yet still there are larger colonies that very well could be what we want. Maybe the antibiotics are not efficient enough, we will try to spread normal plasmid free reuteri from frozen stock on the plates to investigate… We’ll also try a colony screening today with VF2 and VR. Nothing grew on chloramphenicol plates, not even the positive control pVS2, though it grew on erythromycin both when transformed into reuteri and plantarum. Gel electrophoresis: All Pem/Pemp mutagenesises showed bands at the right lengths (~2700 bp), although they were a bit smudgy - weakly stretching backward toward the wells. The digests of Pem simply showed one band, a weaker counterpart of the uncut PCR-product. We suspect the digest didn’t go as supposed, maybe due to the PCR buffer. The lactobacillus colony screenings showed up empty, perhaps the cells didn’t lyse? Or didn’t contain plasmids with VF and VR… We will continue to investigate the resistance problems, and await the sequencing results of the insertsite. The screenings of e. coli transformations done thursday also gave us an empty gel, without even ladders. Possibly the gel had to shallow wells.</div>'

+

}

+

else if(id == 'd2013825')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1> Name of participants: Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Todays work:</h2> Mutagenesis 338.pSB1C3-meffBlue Plasmidprepp: 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin Digest: 147.pSB1C3-B0034-Thioredoxin Ligate: 147.pSB1C3-B0034-Thioredoxin+CP1→ → 346.pSB1C3-CP1-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP8→ →347.pSB1C3-CP8-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP41→ →348.pSB1C3-CP41-B0034-Thioredoxin Transformation: 1.pSB1C3-Red (competens test) 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 285.pSB1C3.J23110-B0034-aeBlue 286.pSB1C3.J23110-B0034-amilCP 287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ 336.pSB1C3-spisPink 337pSB1C3-scOrange 346.pSB1C3-CP1-B0034-Thioredoxin 347.pSB1C3-CP8-B0034-Thioredoxin 348.pSB1C3-CP41-B0034-Thioredoxin 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple Re-streak: 284. pSB1C3-B0034-aeBlue Sequenceprep: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) </div>'

+

}

+

else if(id == 'd2013826')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-26</h1> Name of participants: Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin <h2>Todays work</h2> Streak from frozen stock: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 139. pSB3K3-J23101-B0034-BFP PCR: Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures +2° and +4°C) Slovenian 4CL with His-primers Gel electrophoresis: Erythromycin-cassette PCR-product from Lb2.1 pLUL631 Erythromycin-cassette PCR-product from Lb2.2 pLUL631 Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R Assembly & transformation: 349 Mutagenesis 338.pSB1C3-meffBlue Re streak 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple 343. pSB1C3-J23106-B0034-asCP Plasmidprepp: 102.pSB1C3-CrtE 272.pSB1K3-B0034-Thioredoxin-ZCD-His (272F.pSB1K3-B0034-Thioredoxin-ZCD-His) 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin O/N screen 284. pSB1C3-B0034-aeBlue Screening: 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 346.pSB1C3-CP1-B0034-Thioredoxin 347.pSB1C3-CP8-B0034-Thioredoxin 348. pSB1C3-CP41-B0034-Thioredoxin O/N: 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (annan ligering än ovan, den som screenades) Re-streak 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (den som screenades) Sekvensprep: 102.pSB1C3-CrtE 272.pSB1K3-B0034-Thioredoxin-ZCD-His (272F.pSB1K3-B0034-Thioredoxin-ZCD-His) 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Ligation: 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP1→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP8→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP41→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 20.1.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 → →215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→ → 303.pSB1K3-J23101-B0034-CrtZ 127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→ → 223.pSB3K3-CP8-B0034-UGTCs2-His <h2>Results:</h2> Gel electrophoresis: Erythromycin-cassette PCR-product: Good bands! → proceed with PCR-cleanup and assembly Erythromycin-cassette PCR-product: --”-- Lb9. faecalis JH2-2 pAMbeta1-ori: One of the two had a good band → proceed 77. pSB4C15-red: Unspecific binding → redo PCR with +2° and +4° annealing temp. 269 showed correct bands. 247 and 248 showed credible bands, but a mistake in the assembly was detected (resistance on source of insert is the same as target backbone). These samples were discarded and a new ligation with the backbone pSB1K3 was made. <h2>Characterization:</h2> Western blot sample preparation: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Other experiments</h2> Preparing MRS-agar Preparing low salt LB-agar</div>'

+

}

+

else if(id == 'd2013827')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-08-27</h1> Name of participants: Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> O/N: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 Transformation: Chromoproteins into pSB3K3 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 303.pSB1K3-j23101-B0034-CrtZ 346.pSB1K3-CP1-B0034-Thioredoxin 347.pSB1K3-CP8-B0034-Thioredoxin 348.pSB1K3-CP41-B0034-Thioredoxin Gel electrophoresis: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures +2° and +4°C) Screening PCR: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His Mutagenesis 338.pSB1C3-meffBlue Plasmidprepp: 182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ Sekvensprep: 182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 284. pSB1C3-B0034-aeBlue Re-streak 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple 343. pSB1C3-J23106-B0034-asCP PCR: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures Tm1 and +5°C (with touchdown)) Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 5 µg/ml 10 µg/ml 20 µg/ml 50 µg/ml - lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations: 50 µg/ml 100 µg/ml <h2>Results</h2> Gel electrophoresis: 77. pSB4C15-red +2°C annealing temperature: Strange band at 1000 bp 77. pSB4C15-red +4°C annealing temperature: Strange band at 1000 bp <h2>Other experiments</h2> Preparing test plates, to determine minimum antibiotic concentration: Lb-erythromycin: 5 µg/ml 10 µg/ml 20 µg/ml 50 µg/ml MRS-zeocin: 50 µg/ml 100 µg/ml</div>'

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}

+

else if(id == 'd2013828')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-08-28</h1> Name of participants: Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin <h2>Todays work</h2> Gel electrophoresis: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 77. pSB4C15-red (annealing at Tm1): 77. pSB4C15-red (annealing at Tm1+5): Ligation: 352. pSBLbC3-red (pEL4C15 (ClaI) + pVS2 (ClaI)) 108.pEL3S17-J23106-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-His 141.pEL3S17-J23101-B0034-UGTCs2-His 142.pEL3S17-CP1-B0034-UGTCs2-His 143.pEL3S17-CP8-B0034-UGTCs2-His PCR: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R (Tm1) Restreak 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ Screening O/N: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.B0034-Thioredoxin 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.J23106-B0034-Thioredoxin 202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Digest 36. pEL3S17-red Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Two step Assembly: 92. BBa_J61002-J23110, 149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed 91. BBa_J61002-J23106, 149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed 92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink 91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink 92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple 91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple <h2>Results</h2> Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 5 µg/ml: Growth 10 µg/ml: Growth 20 µg/ml: Growth 50 µg/ml: Still some growth, but less → do new plates with higher conc. and try 50 µg/ml-plates from Erik Gullberg in case our antibiotic has lost efficiency. - lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations: 50 µg/ml: Growth 100 µg/ml: Growth (Plantarum grew less, but did so also on antibiotic free plates.) - lactobacillus (Lb4., Lb11. and Lb12.) onto antibiotic-free MRS-plates: Everything grew on Ab-, plantarum slightly less. Gel electrophoresis: 351.1 pSB1C3-pemp-mut1: A weak band at ~1000 bp 351.2 pSB1C3-pemp-mut1: Nothing 77. pSB4C15-red (annealing at Tm1): Nothing → do a new PCR at TM1. 77. pSB4C15-red (annealing at Tm1+5): Nothing. 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: Failed 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: Failed 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: Failed Re-streak: 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <h2>Other experiments</h2> Preparation of new test-plates: LB-erythromycin: 100 µg/ml 200 µg/ml</div>'

+

}

+

else if(id == 'd2013829')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-29</h1> Name of participants: Mikael Strandgren, Viktor Blomkvist, Alona Nyberg, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> E. coli: 353. pSBLb4E15 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His <h2>Todays work</h2> Transformation: 353. pSBLb4E15 Plasmidprep: 223 346 <h2>Results</h2> Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 100 µg/ml: Weak growth 200 µg/ml: Possibly no growth, let them “grow” some more. <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Other experiments</h2> Preparation of new test-plates: MRS-Zeocin: 250 µg/ml </div>'

+

}

+

else if(id == 'd2013830')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-30</h1> Name of participants: Mikael Strandgren, Viktor Björklund, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> 77. pSB4C15-red 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His Lb9. faecalis JH2-2 pAMbeta <h2>Todays work</h2> Gel extraction: 77. pSB4C15-red (annealing at Tm1): PCR: Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R* *Since the product from monday disappeared. PCR purification: Lb9. faecalis JH2-2 pAMbeta1-ori Digestion: Lb9. faecalis JH2-2 pAMbeta1-ori (PCR product)(XbaI and BglII) 77. pSB4C15-red (PCR product) (NheI and BglII) Ligation: 169. pSBLbEcC2-red (shuttle-vector with pAMbeta and e. coli replicon) <h2>Results</h2> Western blot: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS failed. <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd2013831')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-31</h1> Name of participants: Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> Transformation: 111. pSBLb4C15-red 169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) 386. pSBLbEcC2-red (in PK401 dam-) (pEL+replicon from pAMbeta1) Gel electrophoresis: 169. pSBLbEcC2-red (ligation) Ligation: 111. pSBLb4C15-red (pEL+ replicon from pJP) 352. pSBLbC3-red (pEL+ replicon from pVS2) <h2>Results</h2> Gel electrophoresis: 169. pSBLbEcC2-red (ligation): No band, possibly to weak due to being ligation → transform and screen.</div>'

+

}

+

else if(id == 'd201391')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-09-01</h1> Name of participants: Anders Edlund, Mikael Strandgren, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 275. pSBLb4C15-red (dam-) 352. pSBLbC3-red 406. pSBLbC3-red (dam-) 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> Transformation: 111. pSBLb4C15-red (pEL+ replicon from pJP) 275. pSBLb4C15-red (dam-) 352. pSBLbC3-red (pEL+ replicon from pVS2) 406. pSBLbC3-red (dam-) Screening: 169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) (x2) Re-streak: 169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) <h2>Results</h2> First screening failed (weird gel) <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201392')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-02</h1> Name of participants: Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work: </h2> O/N: 169. pSBLbEcC2-red Mutagenesis PCR: 111.7 pSBLb4C15-red * *with primers to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried). Two attempts, second over night. Ligation: 111. pSBLb4C15-red (pEL4C15+replicon from pJP059)* 352. pSBLbC3-red* *New attempts at ligation, just in case. <h2>Results: </h2> Gel electrophoresis: 111.7 pSBLb4C15-red: No bands Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS failed. <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201393')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-03</h1> Name of participants: Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 261. pSB3K3-J23110-B0034-His-4CL 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work: </h2> Frozen stock and plasmid preparation: 169. pSBLbEcC2-red Gel electrophoresis: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried) Transformation: 111. pSBLb4C15-red (pEL4C15+replicon from pJP059)* *New attempts at ligation, just in case. Sequencing preparation: 169. pSBLbEcC2-red <h2>Characterization experiments:</h2> Western blot sample preparation on construct: 335. pSB3K3-J23101-HIs-STS <h2>Results: </h2> Frozen stock and plasmid preparation: 169. pSBLbEcC2-red: 44.2 ng/µl 169. pSBLbEcC2-red: 47.8 ng/µl Gel electrophoresis: 111.7 pSBLb4C15-red: Correct bands in five lanes of six, weird ladder.</div>'

+

}

+

else if(id == 'd201394')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1> Name of participants: Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 335. pSB3K3-J23101-HIs-STS <h2>Todays work: </h2> Assembly: 353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old) Trasformation: 111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP) 353. pSBLb4E15-red PCR-purification: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried) DpnI-treatment: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'

+

}

+

else if(id == 'd201395')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1> Name of participants: Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L <h2>Ongoing constructs:</h2> 335. pSB3K3-J23101-HIs-STS <h2>Todays work</h2>First day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. <h2>Characterization:</h2> Western blot on construct: 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201396')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1> Name of participants: Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L <h2>Ongoing constructs: </h2> 335. pSB3K3-J23101-HIs-STS <h2>Todays work: </h2> PCR-purification and recircularization: Mutagenesis´d pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30) O/N: 111.7 pSBLb4C15-red* *to do a new plasmid preparation since old may have corrupt red-cassette/contamination) Streak from frozen stock: 111.7 pSBLb4C15-red* * to test if red-cassette has been damaged, or possibly the stock contaminated. Second day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. <h2>Characterization:</h2> Western blot on construct: 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201397')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1> Name of participants: Mikael Strandgren, Christoffer L <h2>Ongoing constructs: </h2> 407. pSB1C3-CP29-Mcherry 408. pSB1C3-CP30-usp45-mCherry 409. pSB1C3-CP29-usp45-mCherry 410. pSB1C3-CP30-tsPurple 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red <h2>Todays work:</h2> Plasmid preparation: 351.5 pSB1C3-pemp-mut1 (mutE) 355.2 pSB3K3-CP29-mCherry 355.3 pSB3K3-CP29-mCherry 357.3 pSB3K3-CP30-usp45-mCherry 357.4 pSB3K3-CP30-usp45-mCherry 358.1 pSB3K3-CP29-usp45-mCherry 358.2 pSB3K3-CP29-usp45-mCherry 363. pSB3K3-CP30-tspurple 364. pSB3K3-CP29-tspurple 365. pSB3K3-CP11-tspurple 366. pSB3K3-CP30-amilCP 367. pSB3K3-CP29-amilCP 368. pSB3K3- CP11-amilCP 369. pSB3K3-CP30-aeblue 370. pSB3K3-CP29-aeblue 371. pSB3K3-CP11-aeblue Assembly: 407. pSB1C3-CP29-Mcherry* 408. pSB1C3-CP30-usp45-mCherry* 409. pSB1C3-CP29-usp45-mCherry* 410. pSB1C3-CP30-tsPurple* 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP* 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue* 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old) * pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today. Transformation 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette. O/N: 111.7 pSBLb4C15-red Third day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. <h2>Results: </h2> O/N: 111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today. Plasmid preparation: 351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl 355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl 355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl 357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl 357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl 358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl 358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl 363. pSB3K3-CP30-tspurple: 75.5 ng/µl 364. pSB3K3-CP29-tspurple: 43.9 ng/µl 365. pSB3K3-CP11-tspurple: 47.3 ng/µl 366. pSB3K3-CP30-amilCP: 74.5 ng/µl 367. pSB3K3-CP29-amilCP: 77.2 ng/µl 368. pSB3K3- CP11-amilCP: 34.3 ng/µl 369. pSB3K3-CP30-aeblue: 37.4 ng/µl 370. pSB3K3-CP29-aeblue: 60.3 ng/µl 371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'

+

}

+

else if(id == 'd201398')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-09-08</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs: </h2> 407. pSB1C3-CP29-Mcherry 408. pSB1C3-CP30-usp45-mCherry 409. pSB1C3-CP29-usp45-mCherry 410. pSB1C3-CP30-tsPurple 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red <h2>Todays work: </h2> Transformations: 411. pSB1C3-CP29-tsPurple * 412. pSB1C3-CP11-tsPurple * 414. pSB1C3-CP29-amilCP * 415. pSB1C3-CP11-amilCP * 417. pSB1C3-CP29-aeBlue * 418. pSB1C3-CP11-aeBlue * * The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good. ON: 353. pSBLB4E15-red <h2>Results: </h2> Transformation 411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today. 412. pSB1C3-CP11-tsPurple: “ 414. pSB1C3-CP29-amilCP “ 415. pSB1C3-CP11-amilCP “ 417. pSB1C3-CP29-aeBlue “ 418. pSB1C3-CP11-aeBlue “ 419. pSBLbEcE-red: Growth covering the plates, only white colonies. pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'

+

}

+

else if(id == 'd201399')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-09</h1> Name of participants: Sabri, Christoffer L <h2>Todays Work:</h2> Digest 338.pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Ligation 338.pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Fourth day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. </div>'

+

}

+

else if(id == 'd2013910')

+

{

+

ds = '<div id="dairy-text"><h1>Tueday 2013-09-10</h1> Name of participants: Sabri, Christoffer L <h2>Todays Work:</h2> Re-streak 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue Fifth day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. </div>'

+

}

+

else if(id == 'd2013911')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-11</h1> Name of participants: Sabri <h2>Todays Work:</h2> O/N: 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue</div>'

+

}

+

else if(id == 'd2013912')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-09-12</h1> Name of participants: Sabri <h2>Todays Work:</h2> Plasmid prep: 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue Sequence prep: 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue</div>'

+

}

+

else if(id == 'd20131013')

+

{

+

ds ='<h1>European Jamboree Lyon end</h1>';

+

}

+

else if(id == 'd20131012')

+

{

+

ds ='<h1>European Jamboree Lyon</h1>';

+

}

+

else if(id == 'd20131011')

+

{

+

ds ='<h1>European Jamboree Lyon start</h1>';

+

}

+

else if(id == 'd2013104')

+

{

+

ds ='<h1>WIKIFREEZE 23.59 Boston time</h1>';

+

}

+

else if(id == 'd2013102')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-10-02</h1> Name of participants: Anders Edlund <h2>Ongoing constructs</h2> Lb47. reuteri 100-23 pSBLbE-red <h2>Todays work</h2> Sequencing preparation: Lb47. reuteri 100-23 pSBLbE-red </div>'

+

}

+

else if(id == 'd2013101')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-10-01</h1> Name of participants: Anders Edlund <h2>Ongoing constructs </h2> Lb47. reuteri 100-23 pSBLbE-red <h2>Todays work</h2> Screening PCR: Lb47. reuteri 100-23 pSBLbE-red <h2>Results</h2> Transformation: To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256: Lb3. pVS2: Growing Lb13. pJP059: Growing 420. pSBLbE-CP11-amilCP: Not growing 435. pSBLbC-CP29-amilCP: Not growing 440. pSBLbC-red: Not growing 441. pSBLbE-red: Growing All negative controls were clear, and the positive controls grew. </div>'

+

}

+

else if(id == 'd2013928')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-28</h1> Name of participants: Viktor Blomkvist, Mikael Strandgren, Erik Edlund <h2>Ongoing constructs</h2> E. coli: 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red L. reuteri: Lb3. pVS2 Lc. lactis: Lb13. pJP059 <h2>Todays work</h2> Transformation: To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256: Lb3. pVS2 Lb13. pJP059 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red </div>'

+

}

+

else if(id == 'd2013927')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-27</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs</h2> Lb4. reuteri 100-23 no plasmid Lb11. Plantarum 256 no plasmid Lb12. Reuteri DSM20016 no plasmid 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red <h2>Todays work</h2> Overnight: Lb4. reuteri 100-23 no plasmid Lb11. Plantarum 256 no plasmid Lb12. Reuteri DSM20016 no plasmid Streak from frozen stock (for photos): 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red </div>'

+

}

+

else if(id == 'd2013924')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-24</h1> Name of participants: Erik Edlund, Mikael Strandgren, Viktor Blomkvist <h2>Ongoing constructs</h2> <h2>Results</h2> Transformation: No growth. Failed negative controls and bad electroporations encourage us to try again when we have time, probably next saturday. </div>'

+

}

+

else if(id == 'd2013921')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-21</h1> Name of participants: Erik Edlund, Mikael Strandgren, Viktor Blomkvist <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red <h2>Todays work</h2> Transformation To L. reuteri DSM 20016 and L. plantarum 256: Lb3. pVS2 (positive control) 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red <h2>Other experiments</h2> Making MRS-agar plates: Erythromycin Chloramphenicol Antibiotic-free </div>'

+

}

+

else if(id == 'd2013920')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-20</h1> Name of participants: Anders Edlund <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP <h2>Todays work</h2> Plasmid preparation: 435. pSBLbC-CP29-amilCP </div>'

+

}

else if(id == 'd2013919')

{

ds ='<h1>Uppsala iGEMs Synbioday, 12.15 to 17.00</h1>';

}

+

else if(id == 'd2013918')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-18</h1> Name of participants: Viktor Blomkvist <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP <h2>Todays work</h2> O/N: 435. pSBLbC-CP29-amilCP </div>'

+

}

+

else if(id == 'd2013917')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-17</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP <h2>Todays work</h2> Re-streak: 435. pSBLbC-CP29-amilCP </div>'

+

}

+

else if(id == 'd2013916')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-16</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP 440. pSBLbC-red <h2>Todays work</h2> Plasmid preparation: 440. pSBLbC-red Assembly and transformation: 435. pSBLbC-CP29-amilCP </div>'

+

}

else

{

@@ Line 1: / Line 1: @@
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 var days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);
-var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385'
+var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385','t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399','t2013910','t2013911','t2013912','t2013917','t2013918','t2013919','t2013920','t2013921','t2013924','t2013927','t2013928','t2013101','t2013102','t2013104','t20131011','t20131012','t20131013');
-,'t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399'
+var inputs = 106;
-,'t2013910','t2013911','t2013912','t2013913','t2013914','t2013915','t2013919');
-var inputs = 96;
 Date.prototype.getWeek = function()
@@ Line 149: / Line 147: @@
    eventString =  '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
    document.getElementById('file_holder').innerHTML = eventString;
+document.getElementById('calendar').style.marginBottom="350px";
   }
@@ Line 186: / Line 185: @@
      eventString = eventString + '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
     }
+document.getElementById('calendar').style.marginBottom="350px";
    document.getElementById('file_holder').innerHTML = eventString;
    eventString = '';
@@ Line 484: / Line 484: @@
 	ds = '<div id="dairy-text"><h1>Wednesday 2013-08-14</h1><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Mikael S, Viktor B, Viktor T, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Peter C, Lovisa P<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>115. pSB3K3-J23101-B0034-His-TAL<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Todays work:</h2><br><b>Re-streak:</b><br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>pSB1K3-J23110-B0034-aeBlue (should be subcloned to pSB1C3 after)<br>pSB3K3-J23110-B0034-AmilCP (should be subcloned to pSB1C3 after)<br>pSB3K3-J23110-B0034-asCP (should be subcloned to pSB1C3 after)<br>Re streak 15/8 or 16/8<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>O/N:</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br><br><b>Streak from frozen stock:</b><br>111. pSBLb4C15-red<br><br><b>Gradient touchdown colony PCR:</b><br>CSP <br><br><b>Gel Electrophoresis:</b><br>CBP (colony PCR from Lb.Reuteri)<br>CSP (colony PCR from Lb.Reuteri)<br>Mcherry (from plasmid preparation)<br>256. pSBLb4C15-pem<br><br><b>PCR purification: </b><br>M1 <br>M2<br>M3<br>M4<br>CB1<br>CB2<br>256. pSBLb4C15-pem (P1)<br><br><b>Digestion:</b><br>M3 (x+p)<br>CB1 (x+p)<br>P1 (x+p)<br>111.7 pSB4C15-red (e+p)<br>Bleomycin resistance cassette (SacI)<br>pEL PCR product with e. coli ori (ClaI)<br>Lb3 reuteri DSM 20016 pVS2 (ClaI)<br>CP promoters with amilGFP, aeBlue,tsPurple<br><br><b>Ligation:</b><br>M3 with pSB1C3, pSBLB4C15 + CP8, CP1<br>CB1 with pSB1C3<br>P1 with pSB1C3<br>pVS2 origin of replication with pEL PCR-product, cut by ClaI <br>CP promoters with amilGFP, aeBlue,tsPurple<br><br><b>Frozen stock and plasmid preparation:</b><br>275.1 pSBLb4C15-red (dam-)*<br>275.2 pSBLb4C15-red (dam-)<br>276. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-)<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-)<br><i>*O/N of clone 1 was made from a very small colony, its colour wasn’t as strong as clone 2’s after growing overnight.</i><br><br><b>Transformation:</b><br>109.pEL3S17-K206000-B0034-IspA x<br>110.pEL3S17-K206001-B0034-IspA x<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His x<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His x<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His x<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His x<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His x<br>184.pSB1C3-B0034-CrtZ x<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His x<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His x<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His x<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His x<br>267.psB3K3-K206000-B0034-IspA x<br>268.psB3K3-K206001-B0034-IspA x<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His x<br>272 pSB1K3-B0034-Thioredoxin-ZCD-His x<br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x<br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x<br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His x<br><br><b>Plasmid prepp:</b><br>280. pSB1A3-B0034-aeBlue (should be subcloned to pSB1C3 after, give Erik the subclones version)<br><br><h2>Results:</h2><br><b>Gel Electrophoresis:</b><br>CBP (colony PCR from Lb.Reuteri)<br>CB1: 54.3o C (NC ok!), CB2: 63.3o C (NC ok!) → Correct bands!!<br>CSP (colony PCR from Lb.Reuteri) <br>CS1: 55.7o C (NC ok!), CS2: 65.6o C (NC ok!) → No bands!! (not even primer dimer) <br>Mcherry (from plasmid preparation)<br>M1: 45.0o C (NC ok!), M2: 47.9o C (NC ok!), M3: 50.5o C (NC ok!), M4: 54.3o C (NC ok!) → Correct bands!!<br>256. pSBLb4C15-pem: <br>P1: 47.9o C (NC ok!) → Correct bands!!<br><br><b>PCR purification: </b><br>M1: 65.9 ng/µl → Store in freezer!<br>M2: 200.6 ng/µl → Store in freezer!<br>M3: 136.8 ng/µl → For digest! → Store in freezer!<br>M4: 49.5 ng/µl → Store in freezer!<br>CB1: 90.8 ng/µl → For digest! → Store in freezer!<br>CB2: 56.7 ng/µl → Store in freezer!<br>P1: 56.2 ng/µl → For digest! → Store in freezer!<br><br><b>Plasmid preparation:</b><br>275.1 PK401 dam- pSBLb4C15-red (dam-):				13.5 ng/µl<br>275.2 PK401 dam- pSBLb4C15-red (dam-):				27.9 ng/µl<br>276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-): 18.3 ng/µl<br>277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-): 26.6 ng/µl<br><br><b>Western blot:</b><br>Transfer overnight not complete but continued with anyway<br>Very promising results, bands can be seen around 50 kDa but the ladder is pretty weak so we can’t be absolutely sure. Looks like the J23101 promotor does’nt work.<br><br><h2>Characterization experiments</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><b>Blotting and development of constructs:</b><br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>115. pSB3K3-J23101-B0034-His-TAL<br><br><h2>Other experiments:</h2><br><b>Making MRS-broth (2x600 ml)</b><br><br><b>New buffers for western blot made </b></div>'
   }
+      else if(id == 'd2013815')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-08-15</h1><br><b>Name of participants: </b> Christoffer Ahlström, Stephanie Herman, Alona Nyberg, Mikael Strandgren, Alexander, Malin, Pontus, Niclas, Christoffer, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H., Peter C, Lovisa P, Theodor L<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Digestions:</b><br>Erythromycin resistance cassette from pLUL631 (SacI, Tango buffer)<br>Bleomycin resistance cassette (SalI, FD buffer)<br><br><b>O/N Screening glycerolstock:</b><br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br><br><b>Re-streak:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>184.pSB1C3-B0034-CrtZ<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>110.pEL3S17-K206001-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br><br><b>Plasmid prep:</b><br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br><br><b>Transformation:</b><br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>292. PK401 dam- pSBLb4C15-CP1-mCherry (dam-)<br>294. PK401 dam- pSBLb4C15-CP8-mCherry (dam-)<br><br><b>Ligations:</b><br>pSBLB4C15 and Bleomycin resistance cassette cut with SacI and SalI<br><br><b>Spreading of strains:</b><br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br><i>E. coli PK401 dam-:</i><br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><b>Screening PCR and gel electrophoresis</b><br><br><h2Results:</h2><br><b>Yesterdays buffers for western blot resulted in smear. </b><br><br><h2>Characterization experiments</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><h2>Other experiments</h2><br><b>Making MRS-agar plates:</b><br>47 Chloramphenicol<br>47 Without antibiotics<br><br><b>Testing yesterdays buffers for western blot.</b><br> <br><b>New separation- and stacking buffers for western blot with a factor 1000 concentration of Tris.</b></div>'
+ }
+      else if(id == 'd2013816')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-16</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Mikael Strandgren, Alexander, Pontus, Nils, Christoffer, Sabri, Hampus.E, Kristoffer L, Victor S, Marcus H, Karl H, Theodor L, Peter C<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>O/N Screening & glycerolstock:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>184.pSB1C3-B0034-CrtZ<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>2 74.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br><br><b>Streak from frozen stock:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br><br><b>Digestion</b><br>Erythromycin cassette amplicon from pLUL631 (SalI)<br><br><b>Ligation</b><br>pSB1C3-pemp<br>298. pSBLb4E15-red (E-cassette amplicon from pLUL631 with pSBLb4C15 (SacI + SalI))<br>297. pSBLb4B15-red (Bleomycin cassette with pSBLb4C15 (SacI + SalI))<br><br><b>Plasmid preparation:</b><br>204.1 pEL3S17-J23110-B0034-HIS-STS<br>204.3 pEL3S17-J23110-B0034-HIS-STS<br>204.4 pEL3S17-J23110-B0034-HIS-STS<br>254.1 pSB1C3-gus<br>254.2 pSB1C3-gus<br>255.1 pSBLB4C15-pemp<br>255.2 pSBLB4C15-pemp<br>255.3 pSBLB4C15-pemp<br>255.4 pSBLB4C15-pemp<br><br><b>O/N:</b><br>204.1 pEL3S17-J23110-B0034-HIS-STS<br>204.3 pEL3S17-J23110-B0034-HIS-STS<br>204.4 pEL3S17-J23110-B0034-HIS-STS<br>254.1 pSB1C3-gus<br>254.2 pSB1C3-gus<br>255.1 pSBLB4C15-pemp<br>255.2 pSBLB4C15-pemp<br>255.3 pSBLB4C15-pemp<br>255.4 pSBLB4C15-pemp<br>257.1 pSB1C3-pemp<br>257.2 pSB1C3-pemp<br>257.3 pSB1C3-pemp<br>280. pSB1K3-J23110-B0034-aeBlue<br>281. pSB3K3-J23110-B0034-amilCP<br>282. pSB3K3-J23110-B0034-asCP<br><br><b>Transformation:</b><br>297. PK401 dam- pSBLb4B15-red <br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>155pSB1C3-j23110-RBS-meffRed<br>156pSB1C3-j23106-RBS-meffRed<br>157pSB1C3-j23110-RBS-spisPink<br>158pSB1C3-j23106-RBS-spisPink<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His <br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His <br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His <br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <br>184.pSB1C3-B0034-CrtZ<br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His <br>247pSB1C3-J23110-RBS-tsPurple<br>248pSB1C3-J23106-RBS-tsPurple<br>249pSB1C3-J23110-RBS-scOrange<br>250pSB1C3-J23106-RBS-scOrange<br>251pSB1C3-J23110-RBS-fwYellow<br>252pSB1C3-J23106-RBS-fwYellow<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>272 pSB1K3-B0034-Thioredoxin-ZCD-His <br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><h2>Results:</h2><br><b>Plasmid prep:</b><br>204.1 pEL3S17-J23110-B0034-HIS-STS: 9.7 ng/µl<br>204.3 pEL3S17-J23110-B0034-HIS-STS: 11.9 ng/µl<br>204.4 pEL3S17-J23110-B0034-HIS-STS: 7.6 ng/µl<br>254.1 pSB1C3-gus: 6.7 ng/µl<br>254.2 pSB1C3-gus: 11.5 ng/µl<br>255.1 pSBLB4C15-pemp: 9.9 ng/µl<br>255.2 pSBLB4C15-pemp: 6.3 ng/µl<br>255.3 pSBLB4C15-pemp: 5.0 ng/µl<br>255.4 pSBLB4C15-pemp: 4.6 ng/µl<br><i>Since the concentrations were so low a new set of overnight cultures were prepared.</i><br><br><b>Spectophotometry:</b><br>Inconclusive results.<br><br><h2>Characterization experiments</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><b>Testing of new buffers for western blot.</b><br><br><h2>Other experiments</h2><br><b>Making MRS-broth (2x600 ml)</b><br><br><b>Making LB-agar plates:</b><br>173 Chloramphenicol <i>(chloramphenicol was forgotten in 26 of the plates, but they had already been mixed with 25 others, so also these had to be discarded. 5 plates of doubtful antibiotic content had already been used by the carotenoid group, they were probably ones with ab. E. colis containing pSB3K3 were spread on some of the mixed plates.)</i><br>28 Kanamycin</div>'
+ }
+      else if(id == 'd2013817')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-17</h1><br><b>Name of participants: </b>Anders Edlund, Alexander, Pontus, Sabri, Hampus.E, Kristoffer L, Viktor @, Marcus H. Ken B-A, Karl H, Theodor L<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Restreak</b><br>254.3 pSB1C3-Gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br>297. pSBLb4B15-red <br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 <br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><b>Plasmid preparation:</b><br>254.1 pSB1C3-Gus<br>254.2 pSB1C3-Gus<br>255.1 pSBLB4C15-pemp<br>255.2 pSBLB4C15-pemp<br>257.1 pSB1C3-pemp<br>257.2 pSB1C3-pemp<br>184.pSB1C3-B0034-CrtZ<br>280. pSB1K3-J23110-B0034-aeBlue<br>281. pSB3K3-J23110-B0034-amilCP<br>282. pSB3K3-J23110-B0034-asCP<br><br><b>Digest:</b><br>147.pSB1C3-B0034-Thioredoxin (X,P)<br><br><b>Ligation:</b><br>147.pSB1C3-B0034-Thioredoxin, 91.BBa_J61002 (amp)-J23106, 36.pEL3S17-red<br>---> 300.pEL3S17-J23106-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin, 92.BBa_J61002 (amp)-J23110, 36.pEL3S17- red<br>---> 301.pEL3S17-J23110-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin, 103.pSB1K3-J23101, 36.pEL3S17-red<br>---> 299.pEL3S17-J23101-B0034-Thioredoxin<br><br><b>Transformation:</b><br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><h2>Results:</h2><br><b>Plasmid preparation:</b><br>254.1 pSB1C3-Gus 110 ng/uL<br>254.2 pSB1C3-Gus 132 ng/uL<br>255.1 pSBLB4C15-pemp156 ng/uL<br>255.2 pSBLB4C15-pemp 158 ng/uL<br>257.1 pSB1C3-pemp 146 ng/uL<br>257.2 pSB1C3-pemp 140 ng/uL</div>'
+ }
+      else if(id == 'd2013818')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-18</h1><br><b>Name of participants: </b>Anders Edlund, Alexander, Pontus, Kristoffer L, Marcus H.<br><br><h2>Ongoing constructs:</h2><br><h2>E. coli:</h2><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>ON:</b><br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br>pSBLB4C15-red<br>pSBLB4C15-red dam-<br><br><b>Restreak:</b><br>68.1 pSB3K3-B0034-BFP<br>68.2 pSB3K3-B0034-BFP<br>68.4 pSB3K3-B0034-BFP<br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.pSB1C3-B0034-Thioredoxin<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His <br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His <br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His <br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.pSB3K3-K206000-B0034-IspA <br>268.pSB3K3-K206001-B0034-IspA <br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.pEL3S17-J23106-B0034-Thioredoxin<br><br><b>PCR:</b> <br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Digest:</b><br>125. pSB1C3-ZCD-His<br>127. pSB1A3-B0034-UGTCs2-His<br>129.pSB1C3-Idi(mut) <br>184.pSB1C3-B0034-CrtZ<br><br><b>Ligation:</b><br>4.pSB1A3-red<br>7.pSB3K3-red<br>36.pEL3S17-red<br>91.j23106<br>92.j23110<br>103.pSB1K3-j23101<br>125. pSB1C3-ZCD-His<br>127. pSB1A3-B0034-UGTCs2-His<br>129.pSB1C3-Idi(mut) <br>184.pSB1C3-B0034-CrtZ<br><br><b>Plasmidprep:</b><br>184.pSB1C3-B0034-CrtZ<br><br><b>Transformation:</b><br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>302. idi (mut)-B0034-Ispa<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br>305.pSB1A3-j23110-B0034-CrtZ<br><br><b>O/N screening:</b><br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><h2>Results:</h2><br><b>Failed:</b><br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <br>184.pSB1C3-B0034-CrtZ<br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His <br>272 pSB1K3-B0034-Thioredoxin-ZCD-His <br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His</div>'
+ }
+      else if(id == 'd2013819')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-19</h1><br><b>Name of participants: </b>Anton Berglund, Viktor Blomkvist, Mikael Strandgren, Stephanie Herman, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alexander, Pontus, Sabri<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Screening</b><br>134.1 pSB1C3-CBP<br>254 pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Screening O/N:</b><br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.B0034-Thioredoxin<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.J23106-B0034-Thioredoxin<br>202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Digestion & screening:</b><br>254.1 pSB1C3-gus: XbaI & PstI<br>254.2 pSB1C3-gus: XbaI & PstI<br>255.1 pSBLB4C15-pemp: EcoRI & PstI<br>255.2 pSBLB4C15-pemp: EcoRI & PstI<br>257.1 pSB1C3-pemp: EcoRI & PstI<br>257.2 pSB1C3-pemp: EcoRI & PstI<br><br><b>Frozen stock:</b><br>134.1 pSB1C3-CBP<br>275. pSBLB4C15-red (dam-)<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>1 ml overnight culture:</b><br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>Plasmid preparation</b><br>111.7(1) pSBLB4C15-red<br>111.7(2) pSBLB4C15-red<br>134.1 pSB1C3-CBP<br>275 pSBLB4C15-red (dam-)<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Overnight culture:</b><br>Lb11.2 plantarum 36E-no plasmid<br><br><b>Phusion PCR:</b><br>usp45 (synthesized signal peptide from Lactococcus lactis)<br><br><b>Restreak</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br><br><b>digest</b><br>36. pEL3S17-red<br><br><b>Ligation</b><br>108.pEL3S17-J23106-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-His<br>141.pEL3S17-J23101-B0034-UGTCs2-His<br>142.pEL3S17-CP1-B0034-UGTCs2-His<br>143.pEL3S17-CP8-B0034-UGTCs2-His<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><b>Two step Assembly:</b><br>92. BBa_J61002-J23110,  149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed<br>91. BBa_J61002-J23106,  149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed<br>92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink<br>91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink<br>92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple<br>91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple<br><br><h2>Results:</h2><br><b>Screening</b><br>134.1 pSB1C3-CBP: showed the correct band!<br>254 pSB1C3-gus: showed a very weak band, not sure if correct!<br>288.1 pSB1C3-pem: showed the correct band!<br>288.2 pSB1C3-pem: showed the correct band!<br>288.3 pSB1C3-pem: did not show the correct band!<br>288.4 pSB1C3-pem: showed the correct band!<br>289.1 pSB1C3-mCherry: showed the correct band!<br>289.2 pSB1C3-mCherry: did not show the correct band!<br>289.3 pSB1C3-mCherry: showed the correct band!<br>289.4 pSB1C3-mCherry: showed the correct band.<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His: fail<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His: fail<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His: fail<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His: fail<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: fail<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: fail<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: fail<br><br><b>Digestion & screening:</b><br>254.1 pSB1C3-gus: XbaI & PstI, did not show the correct bands!<br>254.2 pSB1C3-gus: XbaI & PstI, did not show the correct bands!<br>255.1 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands!<br>255.2 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands!<br>257.1 pSB1C3-pemp: EcoRI & PstI, showed the correct bands!<br>257.2 pSB1C3-pemp: EcoRI & PstI, showed the correct bands!<br><br><b>Plasmid preparation</b><br>111.7(1) pSBLB4C15-red: 81.7 ng/µl<br>111.7(2) pSBLB4C15-red: 93.9 ng/µl<br>134.1 pSB1C3-CBP: 163.8 ng/µl<br>275 pSBLB4C15-red (dam-): 92.4 ng/µl<br>288.1 pSB1C3-pem: 211.2 ng/µl<br>288.2 pSB1C3-pem: 193.2 ng/µl<br>288.4 pSB1C3-pem: 193.9 ng/µl<br>289.1 pSB1C3-mCherry: 230.4 ng/µl<br>289.3 pSB1C3-mCherry: 229.7 ng/µl<br>289.4 pSB1C3-mCherry: 239.7 ng/µl</div>'
+ }
+      else if(id == 'd2013820')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-08-20</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Theodor L, Emil M, Victor S<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Characterisation of the CP promoters with facs:</b><br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>Digestion:</b><br>USP. with E, P<br>191. pSB1A2-fusionGFP with X, P<br>280. pSB1A3-B0034-aeBlue with X, P <br>147.B0034-Thioredoxin (E, S)<br>147.B0034-Thioredoxin (X,P)   <br>27.pEL3K16-red (E,P)<br>125.pSB1C3-ZCD-His (X,P)<br><br><b>Ligation:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Transformation:</b><br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br><br><b>Screen/ ON:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Plasmid prep. / Seq. prep.</b><br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br><br><b>Gel:</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Phusion PCR:</b><br>->219.pSB1C3-Miraculin<br><br><b>Making competent Lactobacillus reuteri</b><br>Lb12.1 reuteri DSM 20016 plasmid free: eight<br>Lb12.2 reuteri DSM 20016 plasmid free: eight<br><br><b>Electroporation</b><br>Lb3.2 reuteri DSM 20016 pVS2: two<br>111.7(1) pSBLB4C15-red: one<br>111.7(2) pSBLB4C15-red: two<br>255.1 pSBLB4C15-pemp: one<br>255.2 pSBLB4C15-pemp: one<br>275 pSBLB4C15-red (dam-): three<br><br><h2>Results:</h2><br><b>Electroporation</b><br>Lb3.2 reuteri DSM 20016 pVS2: one of the two survived<br>111.7(1) pSBLB4C15-red: survived<br>111.7(2) pSBLB4C15-red: one of the two survived<br>255.1 pSBLB4C15-pemp: survived<br>255.2 pSBLB4C15-pemp: survived <br>275 pSBLB4C15-red (dam-): two of the three survived</div>'
+ }
+      else if(id == 'd2013821')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-08-21</h1><br><b>Name of participants: </b>Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Alexander, Pontus, Sabri, Malin, Magnus, christoffer, Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E, Lovisa P<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>204(1-4). pEL3S17-J23110-B0034-His-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Digestion:</b><br>usp45: XbaI & AgeI<br>191.1 pSB1A2-fusionGFP: XbaI, PstI<br>191.1 pSB1A2-fusionGFP: NgoMIV & PstI<br>275. pSBLB4C15-red (dam-): EcoRI & PstI<br>289.1 pSB1C3-mCherry: NgoMIV & PstI<br>289.1 pSB1C3-mCherry: XbaI, PstI<br>297.1 pSBLB4B15-red (dam-): EcoRI & PstI<br><br><b>Ligation:</b><br>pSBLB4B15-CP11-fusionGFP (297.1+63.2+191.1)<br>pSBLB4B15-CP30-fusionGFP (297.1+65.1+191.1)   		 <br>pSBLB4B15-CP11-usp45-fusionGFP (297.1+63.2+usp45+191.1)   <br>pSBLB4B15-CP30-usp45-fusionGFP (297.1+65.2+usp45+191.1)    <br>pSBLB4B15-CP11-usp45-mCherry (297.1+63.2+usp45+289.1)   	 <br>pSBLB4B15-CP30-usp45-mCherry (297.1+65.2+usp45+289.1)   	 <br>pSB1C3-usp45 (1+usp45)<br>pSB1C3-usp45 (6+usp45)<br>pSBLb4C15-CP1-B0034-BFP<br>pSBLb4C15-CP8-B0034-BFP<br>pSBLb4C15-CP11-B0034-BFP<br>pSBLb4C15-CP29-B0034-BFP<br>pSBLb4C15-CP30-B0034-BFP<br>pSBLb4C15-CP41-B0034-BFP<br>pSBLb4C15-CP44-B0034-BFP<br>pSBLb4C15-CP11-aeblue<br>pSBLb4C15-CP30-aeblue<br>pSBLb4B15-CP11-mcherry<br>pSBLb4B15-CP30-mcherry<br>pSBLb4B15-CP11-tspurple<br>pSBLb4B15-CP30-tspurple<br>pSBLb4B15-CP11-amilCP<br>pSBLb4B15-CP30-amilCP<br>pSBLb4B15-CP11-amilGFP<br>pSBLb4B15-CP30-amilGFP<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Transformation:</b><br>108.pEL3S17-J23106-B0034-IspA<br>141.pRL3S17-J23101-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br>204(1-4)<br> 	<br><b>Re-streak:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Screen/ ON:</b><br>219.pSB1C3-Miraculin<br>304.pSB1A3-J23106-B0034-CrtZ<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>280. pSB1A3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP <br><br><b>Plasmid prep. </b><br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br><br><b>Mutagenesis PCR:</b><br>336. pSB1C3-spisPink<br>337. pSB1C3-scOrange<br>338. pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>257. pSB1C3-pemp<br>288. pSB1C3-pem<br><br><h2>Other experiments:</h2><br><b>Phosphorylation of primers (20µl of each):</b><br>Pem-mutE_F<br>Pem-mutE_R<br>Pem-mutS_F<br>Pem-mutS_R<br><br><b>Cleaning of electroporation cuvettes</b></div>'
+ }
+      else if(id == 'd2013822')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-08-22</h1><br><b>Name of participants: </b>Anders Edlund, Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Competent cell preparation:</b><br>Lb11.1 plantarum 36E no plasmid<br>Lb12.1 reuteri DSM 20016 no plasmid<br>Lb12.2 reuteri DSM 20016 no plasmid<br><br><b>Digest:</b><br>147.B0034-Thioredoxin (E, S)<br>147.B0034-Thioredoxin (X,P)   <br>27.pEL3K16-red (E,P)<br>125.pSB1C3-ZCD-His (X,P)<br><br><b>Ligation:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Transformation:</b><br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br><strike>3.2. pVS2 (died in electroporation)<br>310. pSBLB4B15-CP11-fusionGFP (died in electroporation)<br>318. pSBLB4B15-CP11-mCherry (died in electroporation)<br>320. pSBLB4B15-CP11-amilGFP (died in electroporation)<br>313. pSBLB4B15-CP30-usp45-fusionGFP (died in electroporation)<br>317. pSBLB4B15-CP30-tsPurple (died in electroporation)<br>331. pSBLB4C15-CP41-BFP (died in electroporation)<br>332. pSBLB4C15-CP44-BFP (died in electroporation)</strike><br><b>+ other E. coli transformations</b><br><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><b>Screen/ ON:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Plasmid prep. / Seq. prep.</b><br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br><br><b>Gel:</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Phusion PCR:</b><br>->219.pSB1C3-Miraculin<br><br><h2>Results:</h2><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp: Problem with PCR-machine, paused at 98*C for 25 min. → re-do.<br>288. pSB1C3-pem:  				--”--<br><br><b>Transformation:</b><br>11.1 Lb. plantarum 36E:<br>3.2 pVS2: survived electroporation<br>3.2 pVS2: died during electroporation<br>310. pSBLB4B15-CP11-fusionGFP: died during electroporation<br>311. pSBLB4B15-CP30-fusionGFP: survived electroporation<br>312. pSBLB4B15-CP11-usp45-fusionGFP: survived electroporation<br>313. pSBLB4B15-CP30-usp45-fusionGFP: died during electroporation<br>316. pSBLB4B15-CP11-tsPurple: survived electroporation<br>317. pSBLB4B15-CP30-tsPurple: died during electroporation<br><br><b>12.2 Lb. reuteri DSM 20016:</b><br>3.2 pVS2: survived electroporation<br>318. pSBLB4B15-CP11-mCherry: died during electroporation<br>319. pSBLB4B15-CP30-mCherry: survived electroporation<br>320. pSBLB4B15-CP11-amilGFP: died during electroporation<br>321. pSBLB4B15-CP30-amilGFP: survived electroporation<br>322. pSBLB4B15-CP11-amilCP: survived electroporation<br>323. pSBLB4B15-CP30-amilCP: survived electroporation<br>324. pSBLB4C15-CP11-aeBlue: survived electroporation<br><br><b>12.1 Lb. reuteri DSM 20016:</b><br>325. pSBLB4C15-CP30-aeBlue: survived electroporation<br>326. pSBLB4C15-CP1-BFP: survived electroporation<br>327. pSBLB4C15-CP8-BFP: survived electroporation<br>328. pSBLB4C15-CP11-BFP: survived electroporation<br>329. pSBLB4C15-CP29-BFP: survived electroporation<br>330. pSBLB4C15-CP30-BFP: survived electroporation<br>331. pSBLB4C15-CP41-BFP: died during electroporation<br>332. pSBLB4C15-CP44-BFP: died during electroporation<br><br><h2>Characterization:</h2><br><b>Western blot analysis (SDS-page) on constructs:</b><br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15) (neg.contr)<br><br><h2>Other experiments:</h2><br><b>Phosphorylation of primers (60 µl of each):</b><br>Pem-mutE_F<br>Pem-mutE_R<br>Pem-mutS_F<br>Pem-mutS_R</div>'
+ }
+      else if(id == 'd2013823')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-23</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Viktor Berglund, Mikael Strandgren, Anders Edlund, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M<br><br><h2>Ongoing constructs:</h2><br><b>E. coli (D5α):</b><br>111.7 pSBLb4C15-red<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work:</h2><br><b>Gel electrophoresis:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>111.7 pSBLb4C15-red (cut with E and P) (to get reference/examine cutsites)<br><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>Touchdown with Tm1+2 and Tm1+4.<br><br><b>Transformation:</b><br>102.pSB1C3-CrtE<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><b>Re-streak:</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Screening O/N:</b><br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>O/N:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><b>Glycerol stock Plasmidprep/sekvensprep:</b><br>108.pEL3S17-J23106-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>299.pEL3S17-J23101-B0034-Thioredoxin<br><br><h2>Results:</h2><br><b>Gel electrophoresis:</b><br>257. pSB1C3-pemp with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!*<br>288. pSB1C3-pem with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!*<br>111.7 pSBLb4C15-red (cut with E and P): No band<br><i>	*→ redo mutagenesis PCR with higher temperatures</i></div>'
+ }
+      else if(id == 'd2013824')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-24</h1><br><b>Name of participants: </b>Mikael Strandgren, Anders Berglund, Alexander, Pontus, Sabri, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M<br><br><h2>Ongoing constructs:</h2><br><b>Escherichia coli</b><br>257. pSB1C3-pemp<br>288. pSB1C3-pem<br>136. pSB1C3-B0034-USP<br>pSB1C3-B0034-usp45<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><b>Lactobacillus reuteri:</b><br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br><br><b>Lactobacillus plantarum:</b><br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br><br><h2>Todays work:</h2><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue <br><br><b>Digest:</b><br>200.pSB1C3-B0034-asCP E,X<br>148.pSB1C3-RBS-gfasPurple E,X<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 E,X<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI)<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI)<br><br><b>Ligation:</b><br>200.pSB1C3-B0034-asCP+91.BBa_J61002 (amp)-J23106→<br>→ 343.J23106-B0034-asCP<br>148.pSB1C3-RBS-gfasPurple+91.BBa_J61002 (amp)-J23106→<br>→ 160.j23106-RBS-gfasPurple<br>148.pSB1C3-RBS-gfasPurple+92.BBa_J61002 (amp)-J23110→<br>→ 159j23110-RBS-gfasPurple<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1+6.pSB1K3-red→<br>→215.pSB1K3-pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→<br>→ 303.pSB1K3-J23101-B0034-CrtZ<br>127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→<br>→ 223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Transformation:</b><br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>343.pSB1C3-J23106-B0034-asCP<br>160.pSB1C3-j23106-RBS-gfasPurple<br>159.pSB1C3-j23110-RBS-gfasPurple<br>303.pSB1K3-j23101-B0034-CrtZ<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>336.pSB1C3.spisPink<br>337.pSB1C3.scOrange<br>284.pSB1C3.B0034-aeBlue<br>285.pSB1C3.J23110-B0034-aeBlue<br>286.pSB1C3.J23110-B0034-amilCP<br>287.pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>284. pSB1C3-B0034-aeBlue<br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br><br><b>O/N screening:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br>136.<br>312<br>313<br>315<br>321<br>325<br>327<br>332<br>317<br><br><b>O/N:</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Plasmid prepp & sequence prepp</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Gel electrophoresis:</b><br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><i>+ E. coli transformations</i><br><br><b>PCR-purification:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><b>DpnI:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><h2>Results:</h2><br><b>Transformations:</b><br>All transformations spread on Bleomycin/zeocin have grown! If they are the right lactobacilli with the right plasmid remains to be seen, there seem to be a lot of contamination in the form of “clouds” covering all plates. Yet still there are larger colonies that very well could be what we want. Maybe the antibiotics are not efficient enough, we will try to spread normal plasmid free reuteri from frozen stock on the plates to investigate… We’ll also try a colony screening today with VF2 and VR. <br>Nothing grew on chloramphenicol plates, not even the positive control pVS2, though it grew on erythromycin both when transformed into reuteri and plantarum. <br><br><b>Gel electrophoresis:</b><br>All Pem/Pemp mutagenesises showed bands at the right lengths (~2700 bp), although they were a bit smudgy - weakly stretching backward toward the wells. The digests of Pem simply showed one band, a weaker counterpart of the uncut PCR-product. We suspect the digest didn’t go as supposed, maybe due to the PCR buffer. <br>The lactobacillus colony screenings showed up empty, perhaps the cells didn’t lyse? Or didn’t contain plasmids with VF and VR… We will continue to investigate the resistance problems, and await the sequencing results of the insertsite.<br>The screenings of e. coli transformations done thursday also gave us an empty gel, without even ladders. Possibly the gel had to shallow wells.</div>'
+ }
+      else if(id == 'd2013825')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1><br><b>Name of participants:</b> Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work:</h2><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Plasmidprepp:</b><br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Digest:</b><br>147.pSB1C3-B0034-Thioredoxin<br><br><b>Ligate:</b><br>147.pSB1C3-B0034-Thioredoxin+CP1→<br>→ 346.pSB1C3-CP1-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP8→<br>→347.pSB1C3-CP8-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP41→<br>→348.pSB1C3-CP41-B0034-Thioredoxin<br><br><b>Transformation:</b><br>1.pSB1C3-Red (competens test)<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>285.pSB1C3.J23110-B0034-aeBlue<br>286.pSB1C3.J23110-B0034-amilCP<br>287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ<br>336.pSB1C3-spisPink<br>337pSB1C3-scOrange<br>346.pSB1C3-CP1-B0034-Thioredoxin<br>347.pSB1C3-CP8-B0034-Thioredoxin<br>348.pSB1C3-CP41-B0034-Thioredoxin<br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br><br><b>Re-streak:</b><br>284. pSB1C3-B0034-aeBlue<br><br><b>Sequenceprep:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br></div>'
+ }
+      else if(id == 'd2013826')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-26</h1><br><b>Name of participants: </b>Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><h2>Todays work</h2><br><b>Streak from frozen stock:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>PCR:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures +2° and +4°C)</i><br>Slovenian 4CL with His-primers<br><br><b>Gel electrophoresis:</b><br>Erythromycin-cassette PCR-product from Lb2.1 pLUL631<br>Erythromycin-cassette PCR-product from Lb2.2 pLUL631<br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R<br><br><b>Assembly & transformation:</b><br>349<br><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Re streak</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br>343. pSB1C3-J23106-B0034-asCP<br><br><b>Plasmidprepp:</b><br>102.pSB1C3-CrtE<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>(272F.pSB1K3-B0034-Thioredoxin-ZCD-His)<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><b>O/N screen</b><br>284. pSB1C3-B0034-aeBlue<br><br><b>Screening:</b><br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>346.pSB1C3-CP1-B0034-Thioredoxin<br>347.pSB1C3-CP8-B0034-Thioredoxin<br>348. pSB1C3-CP41-B0034-Thioredoxin<br><br><b>O/N:</b><br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (annan ligering än ovan, den som screenades)<br><br><b>Re-streak</b><br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (den som screenades)<br><br><b>Sekvensprep:</b><br>102.pSB1C3-CrtE<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>(272F.pSB1K3-B0034-Thioredoxin-ZCD-His)<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Ligation:</b><br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP1→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP8→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP41→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 20.1.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 → <br>→215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→<br>→ 303.pSB1K3-J23101-B0034-CrtZ<br>127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→<br>→ 223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><h2>Results:</h2><br><b>Gel electrophoresis: </b><br>Erythromycin-cassette PCR-product: Good bands! → proceed with PCR-cleanup and assembly<br>Erythromycin-cassette PCR-product:  			--”--<br>Lb9. faecalis JH2-2 pAMbeta1-ori: One of the two had a good band → proceed<br>77. pSB4C15-red: Unspecific binding → redo PCR with +2° and +4° annealing temp.<br>269 showed correct bands. 247 and 248 showed credible bands, but a mistake in the assembly was detected (resistance on source of insert is the same as target backbone). These samples were discarded and a new ligation with the backbone pSB1K3 was made.<br><br><h2>Characterization:</h2><br><b>Western blot sample preparation:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Other experiments</h2><br><b>Preparing MRS-agar</b><br><br><b>Preparing low salt LB-agar</b></div>'
+ }
+      else if(id == 'd2013827')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday  2013-08-27</h1><br><b>Name of participants: </b>Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>O/N:</b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br><br><b>Transformation:</b><br>Chromoproteins into pSB3K3<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>346.pSB1K3-CP1-B0034-Thioredoxin<br>347.pSB1K3-CP8-B0034-Thioredoxin<br>348.pSB1K3-CP41-B0034-Thioredoxin<br><br><b>Gel electrophoresis:</b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures +2° and +4°C)</i><br><br><b>Screening PCR: </b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Plasmidprepp:</b><br>182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br><br><b>Sekvensprep:</b><br>182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>284. pSB1C3-B0034-aeBlue<br><br><b>Re-streak</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br>343. pSB1C3-J23106-B0034-asCP<br><br><b>PCR:</b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures Tm1 and +5°C (with touchdown))</i><br><br><b>Streaking of plasmid free stock on test plates:</b><br><i>- e. coli (10. and 60.3) onto erythromycin LB-plates:</i><br>5 µg/ml<br>10 µg/ml<br>20 µg/ml<br>50 µg/ml<br><i>- lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations:</i><br>50 µg/ml<br>100 µg/ml<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>77. pSB4C15-red +2°C annealing temperature: Strange band at 1000 bp<br>77. pSB4C15-red +4°C annealing temperature: Strange band at 1000 bp<br><br><h2>Other experiments</h2><br><b>Preparing test plates, to determine minimum antibiotic concentration:</b><br>Lb-erythromycin:<br>5 µg/ml<br>10 µg/ml<br>20 µg/ml<br>50 µg/ml<br><br><b>MRS-zeocin:</b><br>50 µg/ml<br>100 µg/ml</div>'
+ }
+      else if(id == 'd2013828')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday  2013-08-28</h1><br><b>Name of participants: </b>Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><h2>Todays work</h2><br><b>Gel electrophoresis: </b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br>77. pSB4C15-red (annealing at Tm1): <br>77. pSB4C15-red (annealing at Tm1+5): <br><br><b>Ligation: </b><br>352. pSBLbC3-red (pEL4C15 (ClaI) + pVS2 (ClaI))<br>108.pEL3S17-J23106-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-His<br>141.pEL3S17-J23101-B0034-UGTCs2-His<br>142.pEL3S17-CP1-B0034-UGTCs2-His<br>143.pEL3S17-CP8-B0034-UGTCs2-His<br><br><b>PCR: </b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R (Tm1)<br><br><b>Restreak</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br><br><b>Screening O/N:</b><br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.B0034-Thioredoxin<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.J23106-B0034-Thioredoxin<br>202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><br><b>Digest</b><br>36. pEL3S17-red<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><br><b>Two step Assembly:</b><br>92. BBa_J61002-J23110,  149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed<br>91. BBa_J61002-J23106,  149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed<br>92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink<br>91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink<br>92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple<br>91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple<br><br><h2>Results</h2><br><b>Streaking of plasmid free stock on test plates:</b><br>- e. coli (10. and 60.3) onto erythromycin LB-plates:<br>5 µg/ml: Growth <br>10 µg/ml: Growth<br>20 µg/ml: Growth<br>50 µg/ml: Still some growth, but less → do new plates with higher conc. and try 50 µg/ml-plates from Erik Gullberg in case our antibiotic has lost efficiency.<br>- lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations:<br>50 µg/ml: Growth<br>100 µg/ml: Growth (Plantarum grew less, but did so also on antibiotic free plates.)<br>- lactobacillus (Lb4., Lb11. and Lb12.) onto antibiotic-free MRS-plates:<br>Everything grew on Ab-, plantarum slightly less.<br><br><b>Gel electrophoresis: </b><br>351.1 pSB1C3-pemp-mut1: A weak band at ~1000 bp<br>351.2 pSB1C3-pemp-mut1: Nothing<br>77. pSB4C15-red (annealing at Tm1): Nothing → do a new PCR at TM1.<br>77. pSB4C15-red (annealing at Tm1+5): Nothing. <br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: Failed<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: Failed<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: Failed<br><br><b>Re-streak:</b><br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Other experiments</h2><br><b>Preparation of new test-plates:</b><br>LB-erythromycin:<br>100 µg/ml<br>200 µg/ml</div>'
+ }
+      else if(id == 'd2013829')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday  2013-08-29</h1><br><b>Name of participants: </b>Mikael Strandgren, Viktor Blomkvist, Alona Nyberg, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>353. pSBLb4E15<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>353. pSBLb4E15<br><br><b>Plasmidprep:</b><br>223<br>346<br><br><h2>Results</h2><br><b>Streaking of plasmid free stock on test plates:</b><br>- e. coli (10. and 60.3) onto erythromycin LB-plates:<br>100 µg/ml: Weak growth<br>200 µg/ml: Possibly no growth, let them “grow” some more. <br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Other experiments</h2><br><b>Preparation of new test-plates:</b><br>MRS-Zeocin:<br>250 µg/ml<br></div>'
+ }
+      else if(id == 'd2013830')
+ {
+	ds = '<div id="dairy-text"><h1>Friday  2013-08-30</h1><br><b>Name of participants: </b>Mikael Strandgren, Viktor Björklund, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br>77. pSB4C15-red <br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br>Lb9. faecalis JH2-2 pAMbeta<br><br><h2>Todays work</h2><br><b>Gel extraction:</b><br>77. pSB4C15-red (annealing at Tm1):  <br><br><b>PCR:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R*<br>*Since the product from monday disappeared.<br><br><b>PCR purification:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori<br><br><b>Digestion:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori (PCR product)(XbaI and BglII)<br>77. pSB4C15-red (PCR product) (NheI and BglII) <br><br><b>Ligation:</b><br>169. pSBLbEcC2-red (shuttle-vector with pAMbeta and e. coli replicon)<br><br><h2>Results</h2><br><b>Western blot:</b> <br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>failed.<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd2013831')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday  2013-08-31</h1><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br>386. pSBLbEcC2-red (in PK401 dam-) (pEL+replicon from pAMbeta1)<br><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation)<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation): No band, possibly to weak due to being ligation → transform and screen.</div>'
+ }
+      else if(id == 'd201391')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday  2013-09-01</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>275.  pSBLb4C15-red (dam-)<br>352. pSBLbC3-red<br>406. pSBLbC3-red (dam-)<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>275.  pSBLb4C15-red (dam-)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br>406. pSBLbC3-red (dam-)<br><br><b>Screening:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) (x2)<br><br><b>Re-streak:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br><br><h2>Results</h2><br>First screening failed (weird gel)<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201392')
+ {
+	ds = '<div id="dairy-text"><h1>Monday  2013-09-02</h1><br><br><b>Name of participants:</b> Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br><b>O/N:</b><br>169. pSBLbEcC2-red<br><br><b>Mutagenesis PCR:</b><br>111.7 pSBLb4C15-red *<br>*with primers to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried). Two attempts, second over night.<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br>352. pSBLbC3-red*<br>*New attempts at ligation, just in case.<br><br><h2>Results: </h2><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: No bands<br><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>failed.<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201393')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-03</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br>Frozen stock and plasmid preparation:<br>169. pSBLbEcC2-red<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red* <br><i>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</i><br><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br><i>*New attempts at ligation, just in case.</i><br><br><b>Sequencing preparation:</b><br>169. pSBLbEcC2-red<br><br><h2>Characterization experiments:</h2><br><b>Western blot sample preparation on construct:</b><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Results: </h2><br><b>Frozen stock and plasmid preparation:</b><br>169. pSBLbEcC2-red: 44.2 ng/µl<br>169. pSBLbEcC2-red: 47.8 ng/µl<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: Correct bands in five lanes of six, weird ladder.</div>'
+ }
+      else if(id == 'd201394')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>Assembly:</b><br>353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old)<br><br><b>Trasformation:</b><br>111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP)<br>353. pSBLb4E15-red <br><br><b>PCR-purification:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)<br><br><b>DpnI-treatment:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'
+ }
+      else if(id == 'd201395')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1><br><b>Name of participants:</b> Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs:</h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work</h2><b>First day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201396')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>PCR-purification and recircularization:</b><br>Mutagenesis´d pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30)<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red*<br><i>*to do a new plasmid preparation since old may have corrupt red-cassette/contamination)</i><br><br><b>Streak from frozen stock:</b><br>111.7 pSBLb4C15-red*<br><i>* to test if red-cassette has been damaged, or possibly the stock contaminated.</i><br><br><b>Second day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201397')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1><br><br><b>Name of participants:</b> Mikael Strandgren, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work:</h2> <br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE) <br>355.2 pSB3K3-CP29-mCherry<br>355.3 pSB3K3-CP29-mCherry<br>357.3 pSB3K3-CP30-usp45-mCherry<br>357.4 pSB3K3-CP30-usp45-mCherry<br>358.1 pSB3K3-CP29-usp45-mCherry<br>358.2 pSB3K3-CP29-usp45-mCherry<br>363. pSB3K3-CP30-tspurple<br>364. pSB3K3-CP29-tspurple<br>365. pSB3K3-CP11-tspurple<br>366. pSB3K3-CP30-amilCP<br>367. pSB3K3-CP29-amilCP<br>368. pSB3K3- CP11-amilCP<br>369. pSB3K3-CP30-aeblue<br>370. pSB3K3-CP29-aeblue<br>371. pSB3K3-CP11-aeblue<br><br><b>Assembly:</b><br>407. pSB1C3-CP29-Mcherry*<br>408. pSB1C3-CP30-usp45-mCherry*<br>409. pSB1C3-CP29-usp45-mCherry*<br>410. pSB1C3-CP30-tsPurple*<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP* <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue*<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old)<br>* pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today.<br><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>417. pSB1C3-CP29-aeBlue <br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red <br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette.<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red<br><br><b>Third day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Results: </h2><br><b>O/N:</b><br>111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today.<br><br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl<br>355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl<br>355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl<br>357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl<br>357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl<br>358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl<br>358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl<br>363. pSB3K3-CP30-tspurple: 75.5 ng/µl<br>364. pSB3K3-CP29-tspurple: 43.9 ng/µl<br>365. pSB3K3-CP11-tspurple: 47.3 ng/µl<br>366. pSB3K3-CP30-amilCP: 74.5 ng/µl<br>367. pSB3K3-CP29-amilCP: 77.2 ng/µl<br>368. pSB3K3- CP11-amilCP: 34.3 ng/µl<br>369. pSB3K3-CP30-aeblue: 37.4 ng/µl<br>370. pSB3K3-CP29-aeblue: 60.3 ng/µl<br>371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'
+ }
+      else if(id == 'd201398')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday  2013-09-08</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work: </h2><br><b>Transformations:</b><br>411. pSB1C3-CP29-tsPurple *<br>412. pSB1C3-CP11-tsPurple *<br>414. pSB1C3-CP29-amilCP *<br>415. pSB1C3-CP11-amilCP *<br>417. pSB1C3-CP29-aeBlue *<br>418. pSB1C3-CP11-aeBlue *<br>* The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good.<br><br><b>ON:</b><br>353. pSBLB4E15-red<br><br><h2>Results: </h2><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today.<br>412. pSB1C3-CP11-tsPurple: 			“ <br>414. pSB1C3-CP29-amilCP 			“<br>415. pSB1C3-CP11-amilCP 			“<br>417. pSB1C3-CP29-aeBlue 			“<br>418. pSB1C3-CP11-aeBlue			“<br>419. pSBLbEcE-red: Growth covering the plates, only white colonies.<br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'
+ }
+      else if(id == 'd201399')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-09-09</h1><br><b>Name of participants: </b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Digest</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br> <br><b>Ligation</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Fourth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br></div>'
+ }
+      else if(id == 'd2013910')
+ {
+	ds = '<div id="dairy-text"><h1>Tueday 2013-09-10</h1><br><b>Name of participants:</b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Re-streak</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br><br><b>Fifth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br></div>'
+ }
+      else if(id == 'd2013911')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-11</h1><br><b>Name of participants: </b>Sabri<br><br><h2>Todays Work:</h2><br><b>O/N:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'
+ }
+      else if(id == 'd2013912')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-09-12</h1><br><b>Name of participants:</b> Sabri<br><br><h2>Todays Work:</h2><br><b>Plasmid prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br> <br><b>Sequence prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'
+ }
+ else if(id == 'd20131013')
+ {
+  ds ='<h1>European Jamboree Lyon end</h1>';
+ }
+ else if(id == 'd20131012')
+ {
+  ds ='<h1>European Jamboree Lyon</h1>';
+ }
+ else if(id == 'd20131011')
+ {
+  ds ='<h1>European Jamboree Lyon start</h1>';
+ }
+ else if(id == 'd2013104')
+ {
+  ds ='<h1>WIKIFREEZE 23.59 Boston time</h1>';
+ }
+      else if(id == 'd2013102')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-10-02</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Sequencing preparation:</b><br>Lb47. reuteri  100-23 pSBLbE-red<br></div>'
+ }
+       else if(id == 'd2013101')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-10-01</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs </h2><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Screening PCR:</b><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Results</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2: <b>Growing</b><br>Lb13. pJP059: <b>Growing</b><br>420. pSBLbE-CP11-amilCP: Not growing<br>435. pSBLbC-CP29-amilCP: Not growing<br>440. pSBLbC-red: Not growing<br>441. pSBLbE-red: <b>Growing</b><br>All negative controls were clear, and the positive controls grew</b>.<br></div>'
+ }
+       else if(id == 'd2013928')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-28</h1><br><b>Name of participants:</b> Viktor Blomkvist, Mikael Strandgren, Erik Edlund<br><br><h2>Ongoing constructs</h2><br><b>E. coli:</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><b>L. reuteri:</b><br>Lb3. pVS2<br><br><b>Lc. lactis:</b><br>Lb13. pJP059<br><br><h2>Todays work</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2<br>Lb13. pJP059<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'
+ }
+       else if(id == 'd2013927')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-27</h1><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Overnight:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br><br><b>Streak from frozen stock (for photos):</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'
+ }
+       else if(id == 'd2013924')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-24</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br><br><h2>Results</h2><br><b>Transformation</b>:<br>No growth. Failed negative controls and bad electroporations encourage us to try again when we have time, probably next saturday.<br></div>'
+ }
+       else if(id == 'd2013921')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-21</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist <br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Transformation</b><br><i>To L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2 (positive control)<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Other experiments</h2><br><b>Making MRS-agar plates:</b><br>Erythromycin<br>Chloramphenicol <br>Antibiotic-free<br></div>'
+ }
+       else if(id == 'd2013920')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-20</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
   else if(id == 'd2013919')
   {
    ds ='<h1>Uppsala iGEMs Synbioday, 12.15 to 17.00</h1>';
   }
+       else if(id == 'd2013918')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-18</h1><br><b>Name of participants: </b> Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>O/N:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+        else if(id == 'd2013917')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-17</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Re-streak:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+         else if(id == 'd2013916')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-09-16</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>440. pSBLbC-red<br><br><b>Assembly and transformation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
   else
   {

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Latest revision as of 23:26, 4 October 2013