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Team:Paris Bettencourt/Notebook/Drug Screening/Monday 26th August.html
From 2013.igem.org
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| + | <b>P1 phage transduction</b> | ||
| - | + | <br> The p1 phage lysate was prepared as described in the P1 phage transduction protocol. | |
| - | + | <br> BL21 (DE3) <i>ΔCysI Δfpr ΔydbK </i>was grown in 5ml Lb. | |
| - | + | <br>One colony was discovered in transformed plate. This colony was re-streaked and grown overnight. | |
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<br>the ligation product from overnight ligation was used to transorm NEB cc cells by heatshock | <br>the ligation product from overnight ligation was used to transorm NEB cc cells by heatshock | ||
<br>5ul plate gave a few colonies | <br>5ul plate gave a few colonies | ||
<br>10ul plate gave many colonies | <br>10ul plate gave many colonies | ||
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Latest revision as of 23:29, 4 October 2013
P1 phage transduction
The p1 phage lysate was prepared as described in the P1 phage transduction protocol.
BL21 (DE3) ΔCysI Δfpr ΔydbK was grown in 5ml Lb.
One colony was discovered in transformed plate. This colony was re-streaked and grown overnight.
the ligation product from overnight ligation was used to transorm NEB cc cells by heatshock
5ul plate gave a few colonies
10ul plate gave many colonies
