Team:Paris Bettencourt/Notebook/Drug Screening/Friday 30th August.html
From 2013.igem.org
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PCR confirmed that CysI was deleted in BL21(AI) and replaced with a Kanomycin resistance gene. | PCR confirmed that CysI was deleted in BL21(AI) and replaced with a Kanomycin resistance gene. | ||
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Revision as of 00:05, 5 October 2013
Confirmation of P1 phage transduction:
Colony PCR was used on patched colonies from the p1 phage transduction.
PCR confirmed that CysI was deleted in BL21(AI) and replaced with a Kanomycin resistance gene.