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Template:Team:Bonn:NetworkData
From 2013.igem.org
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content.titleLong = "Operon-model"; | content.titleLong = "Operon-model"; | ||
content.summary= "The operon-model is a very popular one. The operon allows an organism to regulate the expression of specific genes and therefore the production of corresponding proteins, depending on the concentration of a specific substrate ("substrate-induction") or the lack of an important product ("product-repression")."; | content.summary= "The operon-model is a very popular one. The operon allows an organism to regulate the expression of specific genes and therefore the production of corresponding proteins, depending on the concentration of a specific substrate ("substrate-induction") or the lack of an important product ("product-repression")."; | ||
| - | content.text= "In absence of the substrate, a special repressor binds to the operator DNA sequence in order to inhibit the transcription by the DNA polymerase. The substrate can bind to an allosteric center of the repressor, which leads to a change of conformation. Now the repressor lost his affinity to the operator DNA and cannot inhibit the transcription anymore. This is called "substrate induction".<sup> <a href=#711>71.1</a> </sup> </br> </br><div class='content-imgage'> <img src=https://static.igem.org/mediawiki/2013/0/0f/BonnLacOperon.jpg></br><i>lac </i>operon<sup><a href=#712>71.2</a></sup> </br> <a href='geneticsengineering.blogspot.com'> </a> </div> </br> </br> "Product repression" works the other way around. As long as there is an excess of substrate and a lack of product, the repressor has an low affinity to the operator DNA and | + | content.text= "In absence of the substrate, a special repressor binds to the operator DNA sequence in order to inhibit the transcription by the DNA polymerase. The substrate can bind to an allosteric center of the repressor, which leads to a change of conformation. Now the repressor lost his affinity to the operator DNA and cannot inhibit the transcription anymore. This is called "substrate induction".<sup> <a href=#711>71.1</a> </sup> </br> </br><div class='content-imgage'> <img src=https://static.igem.org/mediawiki/2013/0/0f/BonnLacOperon.jpg></br><i>lac </i>operon<sup><a href=#712>71.2</a></sup> </br> <a href='geneticsengineering.blogspot.com'> </a> </div> </br> </br> "Product repression" works the other way around. As long as there is an excess of substrate and a lack of product, the repressor has an low affinity to the operator DNA and is not able to inhibit the transcription. If there is enough product, it can bind to the allosteric center of the repressor, which improves it's affinity to the operator DNA. Now it is able to inhibit transcription.<sup> <a href=#713>71.3</a> </sup></br> </br><div class='content-image'> <img src='https://static.igem.org/mediawiki/2013/e/ed/BonnTrpOperon.jpg'></br>Tryptophan operon<sup><a href=#714>71.4</a></sup></div></br> </br></br><p><a name=711>71.1 </a> <a href='http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722110/'>A single mutation in the core domain of the lac repressor reduces leakiness, Pietro Gatti-Lafranconi, Willem P Dijkman, Sean RA Devenish, and Florian Hollfelder corresponding author.</a></p><p><a name=712>71.2</a> <a href='http://geneticsengineering.blogspot.de/'>geneticsengineering.blogspot </a></p><p><a name=713>71.3</a> <a href='http://www.ncbi.nlm.nih.gov/pubmed/22713856'> Influence of the feedback loops in the trp operon of B. subtilis on the system dynamic response and noise amplitude. Zamora-Chimal C, Santillán M, Rodríguez-González J.</a></p><p><a name=714>71.4</a> <a href='http://bio1903.nicerweb.com/Locked/media/ch18/18_21bTrpOperon_2.jpg'> bio1903.nicerweb.com/Locked/media/ch18/18_21bTrpOperon_2.jpg </a>"; |
content.type="Background"; | content.type="Background"; | ||
break; | break; | ||
Revision as of 00:22, 5 October 2013
