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Team:Paris Saclay/Notebook/June/28
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='''Notebook : June 28'''= | ='''Notebook : June 28'''= | ||
=='''Lab work'''== | =='''Lab work'''== | ||
| - | + | ==='''Objective : obtaining BBa_K1155000'''=== | |
| - | - | + | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''=== |
| - | - | + | ===='''1 - PCR for the sequence of promoter Pndh* and origin of replication''' ==== |
| - | - | + | - Make an agarose gel with BET |
| - | - | + | - Gel electrophoresis with PCR product |
| - | - | + | ==== '''2 - Test efficiency of the super competent cell''' ==== |
| + | |||
| + | - transformation super competent cells with plasmid PUC18. (Spread bacteria on the boxes) | ||
| + | |||
| + | Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial tranformation]] | ||
| + | |||
| + | - Plate bacteria on LB plate with ampicillin | ||
| + | |||
| + | |||
| + | |||
Latest revision as of 01:02, 5 October 2013
Contents |
Notebook : June 28
Lab work
Objective : obtaining BBa_K1155000
A - Aerobic/Anaerobic regulation system / B - PCB sensing system
1 - PCR for the sequence of promoter Pndh* and origin of replication
- Make an agarose gel with BET
- Gel electrophoresis with PCR product
2 - Test efficiency of the super competent cell
- transformation super competent cells with plasmid PUC18. (Spread bacteria on the boxes)
Protocol : Bacterial tranformation
- Plate bacteria on LB plate with ampicillin
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