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Team:Leeds/Project
From 2013.igem.org
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type="image/png" | type="image/png" | ||
href="https://2013.igem.org/File:Leeds_favicon.png"> | href="https://2013.igem.org/File:Leeds_favicon.png"> | ||
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| + | /************ Gallery javascript inserts for header ************/ | ||
| + | <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.6.0/jquery.min.js"></script> | ||
| + | |||
| + | <style type="text/css"> | ||
| + | |||
| + | /*Make sure your page contains a valid doctype at the top*/ | ||
| + | #simplegallery1{ //CSS for Simple Gallery Example 1 | ||
| + | position: relative; /*keep this intact*/ | ||
| + | visibility: hidden; /*keep this intact*/ | ||
| + | border: 8px solid black; | ||
| + | } | ||
| + | |||
| + | #simplegallery1 .gallerydesctext{ //CSS for description DIV of Example 1 (if defined) | ||
| + | text-align: left; | ||
| + | padding: 2px 5px; | ||
| + | font-family: AlteHaasGrotesk, Verdana; | ||
| + | } | ||
| + | |||
| + | </style> | ||
| + | |||
| + | <script type="text/javascript" src="simplegallery.js"> | ||
| + | |||
| + | /*********************************************** | ||
| + | * Simple Controls Gallery- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com) | ||
| + | * This notice MUST stay intact for legal use | ||
| + | * Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more | ||
| + | ***********************************************/ | ||
| + | |||
| + | </script> | ||
| + | |||
| + | <script type="text/javascript"> | ||
| + | |||
| + | var Explanagallery=new simpleGallery({ | ||
| + | wrapperid: "explanagallery", //ID of main gallery container, | ||
| + | dimensions: [750, 380], //width/height of gallery in pixels. Should reflect dimensions of the images exactly | ||
| + | imagearray: [ | ||
| + | ["http://i26.tinypic.com/11l7ls0.jpg", "http://en.wikipedia.org/wiki/Swimming_pool", "_new", "There's nothing like a nice swim in the Summer."], | ||
| + | ["http://i29.tinypic.com/xp3hns.jpg", "http://en.wikipedia.org/wiki/Cave", "", ""], | ||
| + | ["http://i30.tinypic.com/531q3n.jpg", "", "", "Eat your fruits, it's good for you!"], | ||
| + | ["http://i31.tinypic.com/119w28m.jpg", "", "", ""] | ||
| + | ], | ||
| + | autoplay: [true, 5000, 30], //[auto_play_boolean, delay_btw_slide_millisec, cycles_before_stopping_int] | ||
| + | persist: false, //remember last viewed slide and recall within same session? | ||
| + | fadeduration: 500, //transition duration (milliseconds) | ||
| + | oninit:function(){ //event that fires when gallery has initialized/ ready to run | ||
| + | //Keyword "this": references current gallery instance (ie: try this.navigate("play/pause")) | ||
| + | }, | ||
| + | onslide:function(curslide, i){ //event that fires after each slide is shown | ||
| + | //Keyword "this": references current gallery instance | ||
| + | //curslide: returns DOM reference to current slide's DIV (ie: try alert(curslide.innerHTML) | ||
| + | //i: integer reflecting current image within collection being shown (0=1st image, 1=2nd etc) | ||
| + | } | ||
| + | }) | ||
| + | |||
| + | </script> | ||
</head> | </head> | ||
</html> | </html> | ||
Revision as of 15:09, 15 July 2013
/************ Gallery javascript inserts for header ************/
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Phase IWe split our project into self-contained phases, to help better organise ourselves, but also lay out a road map for our own future work within and without iGEM. The first of these looks at developing, characterising and producing basic Devices. Device I Device I is a very simple modification of the Cpx promoter in E. coli to produce a fluorescent reporter when the cell is under membrane stress. We will then characterise and test the insertion following the work by [http://www.ncbi.nlm.nih.gov/pubmed/11830644 Otto & Silhaevy 2001] in which glass beads were used to induce hydrophobic membrane stress. What we would hope is that when we add our cells to a vial containing similar hydrophobic beads we can detect fluorescence emissions confirming that our biobrick is working as we had hoped. Bio bricks we will be using:
BBa_K135000 pCpxR promoter responds to membrane stress
BBa_K081012 Green fluorescent protein generator. Contain ribosome binding site and terminator
Device 2 Simultaneously, we will be developing a BioBrick for physical attachment and detection of particles. This will work using Ice Nucleation Protein to display a oligo-peptide of our choice on the outer-membrane of our E. coli, initially this will be a peptide capable of binding silica beads allowing us to create a model system of particle detection. The cells producing this receptor complex will also be constitutively producing a fluorescent reporter of our choice to allow for its detection on silica beads following removal of supernatant and washing. Biobricks we will be using: BBa_K081012 Green fluorescent protein generator. Contain ribosome binding site and terminator BBa_K523008 Ice nucleation protein. Transported to cell membrane shows C-terminus on cell surface BBaa_B0015 Double terminator BBa_B0034 Strong ribosome binding site BBa_J23119 Constitutive promoter Again, fluorescent reporters will be used, but this time the focus is on the adhesion proteins being used.
Youtube VideosWhile doing all of this, the team aims to create short [http://www.youtube.com/user/igemleeds/videos Youtube] videos to teach Synthetic Biology at a range of levels, but especially including instructional videos for future team members.
Phase IIPhase II takes the products of Phase I to the next level, and begins to look at integration of the two BioBricks. Device III Due to it's size, the team have decided to collaborate with the Evry team and make use of the Goldengate/GoldenBrick assembly methods. This will hopefully allow our small team to rather rapidly integrate and produce this thrid master Device.
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