Team:Bonn/Project/

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       <img src="https://static.igem.org/mediawiki/2013/7/79/Bonn_sponsors_limes.gif" class="bottom-sponsor" height="50px" id="sponsor-limes">
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       <div class="subpage-head-link"><a href="javascript:history.back();">Back</a></br> <span id="type">Category: Project</span></div>
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= Overview =
 
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            Making versatile control of biological machines easily possible for everybody we engineer a system for
 
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            light dependend control of protein activity per protein degradation.</br>
 
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            Using the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused
 
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            protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is
 
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            activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br>
 
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            Modelling of the light dependency of protein degradation will even make simple and accurate control of
 
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            protein activity for any desired level of activity possible.</br>
 
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            As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.</br>
 
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Latest revision as of 02:17, 5 October 2013

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