"
Team:Grenoble-EMSE-LSU/Documentation/Biobricks
From 2013.igem.org
(Difference between revisions)
| Line 35: | Line 35: | ||
<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141001> BBa_K1141001 </a></h3> | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141001> BBa_K1141001 </a></h3> | ||
| - | <p> align="center"><img src="https://static.igem.org/mediawiki/2013/7/7b/Grenoble_2013_KR1.png" width="350"></p><br> | + | |
| + | <p> align="center"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/7/7b/Grenoble_2013_KR1.png" width="350"></p> | ||
| + | <br> | ||
<p><strong>pLac-RBS-KillerRed</strong> | <p><strong>pLac-RBS-KillerRed</strong> | ||
| - | <br>This BioBrick was obtained by PCR on an eukaryote vector containing the coding sequence of the <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">KillerRed </a> protein (generous gift of Stefan Dimitrov and Yohan Roulland, IAB Grenoble). A PCR allowed to flank it with the restriction sites KpnI and BamHI upstream and downstream. It was then ligated into the pQE30 vector(Qiagen), which contains a pLac promoter and RBS. | + | <br> |
| + | This BioBrick was obtained by PCR on an eukaryote vector containing the coding sequence of the <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">KillerRed </a> protein (generous gift of Stefan Dimitrov and Yohan Roulland, IAB Grenoble). A PCR allowed to flank it with the restriction sites KpnI and BamHI upstream and downstream. It was then ligated into the pQE30 vector(Qiagen), which contains a pLac promoter and RBS. | ||
<br> | <br> | ||
The <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">KillerRed </a> protein fits in our project in 2 ways: its fluorescence makes the transformed cells easily detectable and can be used to quantify the amount of live cells by our device. Its ROS-mediated killing is triggered by light, such that no chemical product has to be added in the medium to make it work and the process can be stopped as soon as the light is switched off. | The <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">KillerRed </a> protein fits in our project in 2 ways: its fluorescence makes the transformed cells easily detectable and can be used to quantify the amount of live cells by our device. Its ROS-mediated killing is triggered by light, such that no chemical product has to be added in the medium to make it work and the process can be stopped as soon as the light is switched off. | ||
Revision as of 03:31, 5 October 2013
