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Team:Grenoble-EMSE-LSU/Documentation/Biobricks
From 2013.igem.org
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<h1>Data Page</h1> | <h1>Data Page</h1> | ||
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<h2> Our parts</h2> | <h2> Our parts</h2> | ||
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| - | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141001 | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141001"> BBa_K1141001 </a></h3> |
| - | <p> align="center"><img src="https://static.igem.org/mediawiki/2013/7/7b/Grenoble_2013_KR1.png" width="350"></p><br> | + | |
| + | <p> <align="center"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/7/7b/Grenoble_2013_KR1.png" width="350"></p> | ||
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<p><strong>pLac-RBS-KillerRed</strong> | <p><strong>pLac-RBS-KillerRed</strong> | ||
| - | <br>This BioBrick was obtained by PCR on an eukaryote vector containing the coding sequence of the <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">KillerRed </a> protein (generous gift of Stefan Dimitrov and Yohan Roulland, IAB Grenoble). A PCR allowed to flank it with the restriction sites KpnI and BamHI upstream and downstream. It was then ligated into the pQE30 vector(Qiagen), which contains a pLac promoter and RBS. | + | <br> |
| + | This BioBrick was obtained by PCR on an eukaryote vector containing the coding sequence of the <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">KillerRed </a> protein (generous gift of Stefan Dimitrov and Yohan Roulland, IAB Grenoble). A PCR allowed to flank it with the restriction sites KpnI and BamHI upstream and downstream. It was then ligated into the pQE30 vector(Qiagen), which contains a pLac promoter and RBS. | ||
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The <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">KillerRed </a> protein fits in our project in 2 ways: its fluorescence makes the transformed cells easily detectable and can be used to quantify the amount of live cells by our device. Its ROS-mediated killing is triggered by light, such that no chemical product has to be added in the medium to make it work and the process can be stopped as soon as the light is switched off. | The <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">KillerRed </a> protein fits in our project in 2 ways: its fluorescence makes the transformed cells easily detectable and can be used to quantify the amount of live cells by our device. Its ROS-mediated killing is triggered by light, such that no chemical product has to be added in the medium to make it work and the process can be stopped as soon as the light is switched off. | ||
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| - | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141002><h3>BBa_K1141002</h3></a> | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141002"><h3>BBa_K1141002</h3></a> |
| - | <p> align="center"><img src="https://static.igem.org/mediawiki/2013/7/7b/Grenoble_2013_KR1.png" width="350"></p> | + | <p> <align="center"><img src="https://static.igem.org/mediawiki/2013/7/7b/Grenoble_2013_KR1.png" width="350"></p> |
<p><strong>pLac-RBS-KillerRed</strong> | <p><strong>pLac-RBS-KillerRed</strong> | ||
<br>This BioBrick was obtained from BBa_K1141001 using PCR to amplify the part pLac-RBS-KillerRed. An overlapping PCR was performed to remove an EcoRI restriction site from the sequence in between pLac and RBS. The sequence was then flanked with the iGEM suffix and prefix.</p> | <br>This BioBrick was obtained from BBa_K1141001 using PCR to amplify the part pLac-RBS-KillerRed. An overlapping PCR was performed to remove an EcoRI restriction site from the sequence in between pLac and RBS. The sequence was then flanked with the iGEM suffix and prefix.</p> | ||
| - | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141000><h3>BBa_K1141000</h3></a> | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141000"><h3>BBa_K1141000</h3></a> |
| - | <p> align="center"><img src="https://static.igem.org/mediawiki/2013/3/33/Grenoble_2013_MCherry1.png" width="350"></p> | + | <p> <align="center"><img src="https://static.igem.org/mediawiki/2013/3/33/Grenoble_2013_MCherry1.png" width="350"></p> |
<p><strong>pLac-RBS-mCherry</strong> | <p><strong>pLac-RBS-mCherry</strong> | ||
<br>This part contains the BioBricks <a href="http://parts.igem.org/Part:BBa_R0010">BBa_R0010</a> and <a href "http://parts.igem.org/Part:BBa_J06702">BBa_J06702</a>. | <br>This part contains the BioBricks <a href="http://parts.igem.org/Part:BBa_R0010">BBa_R0010</a> and <a href "http://parts.igem.org/Part:BBa_J06702">BBa_J06702</a>. | ||
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| - | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141003><h3>BBa_K1141003</h3></a> | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141003"><h3>BBa_K1141003</h3></a> |
| - | <p> align="center"><img src="https://static.igem.org/mediawiki/2013/a/ab/Grenoble_Gnsns_GFP.png" width="350"></p> | + | <p> <align="center"><img src="https://static.igem.org/mediawiki/2013/a/ab/Grenoble_Gnsns_GFP.png" width="350"></p> |
<p><strong>Green sensor-GFP</strong> | <p><strong>Green sensor-GFP</strong> | ||
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| - | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141004><h3>BBa_K1141004</h3></a> | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141004"><h3>BBa_K1141004</h3></a> |
| - | <p> align="center"><img src="https://static.igem.org/mediawiki/2013/8/80/Grenoble_Redsns_rfp.png" width="350"></p> | + | <p> <align="center"><img src="https://static.igem.org/mediawiki/2013/8/80/Grenoble_Redsns_rfp.png" width="350"></p> |
<p><strong>Red sensor-RFP</strong> | <p><strong>Red sensor-RFP</strong> | ||
<br>Built by Gibson Assembly with samples of vectors sent by Pr.Voigt lab. | <br>Built by Gibson Assembly with samples of vectors sent by Pr.Voigt lab. | ||
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| - | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141005><h3>BBa_K1141005</h3></a> | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141005"><h3>BBa_K1141005</h3></a> |
| - | <p> align="center"><img src="https://static.igem.org/mediawiki/2013/7/7b/Grenoble_Redsns_KR.png" width="350"></p> | + | <p> <align="center"><img src="https://static.igem.org/mediawiki/2013/7/7b/Grenoble_Redsns_KR.png" width="350"></p> |
<p><strong>Red Sensor-KillerRed</strong> | <p><strong>Red Sensor-KillerRed</strong> | ||
<br>Built by Gibson Assembly with samples of vectors sent by Pr.Voigt lab. | <br>Built by Gibson Assembly with samples of vectors sent by Pr.Voigt lab. | ||
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| - | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141006><h3>BBa_K1141006</h3></a> | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1141006"><h3>BBa_K1141006</h3></a> |
| - | <p> align="center"><img src="https://static.igem.org/mediawiki/2013/1/15/Grenoble_2013_Sspb.png" width="350"></p> | + | <p> <align="center"><img src="https://static.igem.org/mediawiki/2013/1/15/Grenoble_2013_Sspb.png" width="350"></p> |
<p><strong>pBad-RBS-sspB</strong> | <p><strong>pBad-RBS-sspB</strong> | ||
<br>This part contains the BioBricks <a href="http://parts.igem.org/Part:BBa_K206000"> pBAD K206000</a> and sspB <a href="http://parts.igem.org/Part:BBa_K174000">K174000</a>, the RBS has been added via PCR on sspB, the rest of the BioBrick was built by standard assembly. | <br>This part contains the BioBricks <a href="http://parts.igem.org/Part:BBa_K206000"> pBAD K206000</a> and sspB <a href="http://parts.igem.org/Part:BBa_K174000">K174000</a>, the RBS has been added via PCR on sspB, the rest of the BioBrick was built by standard assembly. | ||
Latest revision as of 03:35, 5 October 2013
