Team:METU Turkey

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Taking a major role in pollination, bees are one of the most important organisms within an ecosystem. However their populations are in serious decline. Colony Collapse Disorder has been found as the most common cause of the disappearance of bees in large numbers. Some pathogens like parasitic fungi and a pesticide called imidacloprid are the leading reasons of CCD. In this study, our aim is to decrease the number of hives affected by chemical compounds such as imidacloprid.  
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<img class="grpelem" id="u5138-13" src="http://ghucuk.netii.net/u5138-13.png" alt="Our Project Description:

 Taking a major role in pollination, bees are one of the most important organisms within an ecosystem. However their populations are in serious decline. Colony Collapse Disorder has been found as the most common cause of the disappearance of bees in large numbers. Some pathogens like parasitic fungi and a pesticide called imidacloprid are the leading reasons of CCD. In this study, our aim is to decrease the number of hives affected by chemical compounds such as imidacloprid. Our plan is to turn the mutualistic bacteria living in bees' guts into a shield mechanism to protect the bees against these factors. A protein CYP6G1 found in Drosophilia melanogaster has the ability to degrade imidacloprid into harmless substances. Moreover, coumaric acid increases the general immunity of bees against harmful components and parasitic organisms and we aim to increase the level of coumaric acid in bees' guts. The main objective of this study is the transformation of the genes coding for these two proteins to Bacillus subtilis, which mutualistically live in bees' guts." width="449" height="288"/><!-- rasterized frame -->
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<p id="u3287-4"><span><span class="actAsDiv normal_text" id="u3288"><!-- content --><span class="actAsDiv museBGSize" id="u3289"><!-- image --></span></span></span><span>&nbsp; </span><span id="u3287-3">As a well&#45;known fact, bees take the major part in pollination, which leads to give fruit, while preparing honey. During the last few decades, some powerful insecticides like imidacloprid were developed to fight with harmful insects; however, the ability to kill almost all insects started to affect other insects, which they must not have affected from the beginning.</span></p>
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<p id="u3287-6">This was the start point of our project. We, as a group formed by mostly biological sciences students, knew that no ecological system can maintain without bees. When we looked into the source of the problem, it was clearly revealed that we couldn’t stop usage of imidacloprid. Then, we came up with an idea to protect bees against existing imidacloprid. Having known that drosophila has some sort of immunity to this insecticide, we planned to use it.</p>
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<p id="u19146-3"><span>&nbsp; </span><span id="u19146-2">To protect the bees against living microorganisms that are dangerous for the bees is our aim. We made a research and we realized that bees recently suffer from insufficient feed.</span></p>
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<p>Since beekeepers prefer to feed the bees with high&#45;fructose corn syrup or sucrose because of the low prices, bees started to suffer from insufficient nutrition. Bees lost their ability of fighting against the pathogens, parasites and pesticides and this situation creates a major problem, colony losses. P&#45;coumaric acid is the most important substance as one of these nutritions.</p>
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<p>P&#45;coumaric acid, a monomer of sporopollenin, is the essential substance for the bees’ diet.</p>
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<p id="u3292-2"><span id="u3292">&nbsp;In this year, in the kill switch part of our project, we use an mRNA interferase, MazF, which cleaves mRNA’s at specific sequences. In our kill switch, we used anti&#45;sense RNA principle as a template. According to this principle, MazF, which is constantly produced via a constitutive promoter, is got inactivated by Anti&#45;MazF construct. In order to trigger this mechanism, we used IPTG, a harmless molecule for the bee which at the same time does not appear in the honey too. When IPTG is present in the environment, LacI is inhibited by IPTG and therefore promoter gets activated. With the activated promoter of it, Anti&#45;MazF is produced and inactive MazF. As long as IPTG exists, MazF gets inactivated continuously; therefore, the bacteria maintain their lives. On the other hand, if bacteria exists in a IPTG&#45;free environment, Anti&#45;MazF producing stops, which leads to MazF producing and bacteria get killed by MazF.</span></p>
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<p id="u3292-4">In this circuit, we used the parts K143053 as a constitutive promoter and SpoVG RBS due to their more efficient works in Bacillus subtilis. Besides these two, we used the part I732820 to produce LacI, but we had to change the RBS of this system since RBSelowitz is less effective in Bacillus then RBSspoVG. With this change in the activity of the RBS, we can compete with the production of MazF.</p>
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<p id="u3292-5">&nbsp;</p>
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<p id="u3292-6">&nbsp;</p>
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<p>Imidacloprid Immunity</p>
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<p>Immune System Boost</p>
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<p>Kill Switch</p>
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<a class="twitter-timeline" href="https://twitter.com/iGEM_METU2013" data-widget-id="372850246176362496">Tweets by @iGEM_METU2013</a>
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Our plan is to turn the mutualistic bacteria living in bees' guts into a shield mechanism to protect the bees against these factors. A protein CYP6G1 found in <i>Drosophilia melanogaster</i> has the ability to degrade imidacloprid into harmless substances. Moreover, coumaric acid increases the general immunity of bees against harmful components and parasitic organisms and we aim to increase the level of coumaric acid in bees' guts. The main objective of this study is the transformation of the genes coding for these two proteins to Bacillus subtilis, which mutualistically live  in bees' guts.
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<!--- The Mission, Experiments --->
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!align="center"|[[Team:METU_Turkey|Home]]
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!align="center"|[[Team:METU_Turkey/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=METU_Turkey Official Team Profile]
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!align="center"|[[Team:METU_Turkey/Project|Project]]
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!align="center"|[[Team:METU_Turkey/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:METU_Turkey/Modeling|Modeling]]
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!align="center"|[[Team:METU_Turkey/Notebook|Notebook]]
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<div class="SlideShowCaptionPanel clearfix" id="u6679"><!-- stack box -->
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<div class="SSSlideCaption clearfix grpelem" id="u7304-4"><!-- content -->
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<p>First meeting of METU iGEM '13 team</p>
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</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7007-4"><!-- content -->
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<p>It was a snowy day when we fell in love with iGEM.</p>
 +
</div>
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<div class="SSSlideCaption invi clearfix grpelem" id="u7016-4"><!-- content -->
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<p>The aseptic conditions are satisfied.</p>
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<div class="SSSlideCaption invi clearfix grpelem" id="u7025-4"><!-- content -->
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<p>Researching for our project.</p>
 +
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<div class="SSSlideCaption invi clearfix grpelem" id="u7034-4"><!-- content -->
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<p>Optimizations for lab work</p>
 +
</div>
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<div class="SSSlideCaption invi clearfix grpelem" id="u7043-4"><!-- content -->
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<p>Collaboration with ITU iGEM team @ Istanbul</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7052-4"><!-- content -->
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<p>Collaboration with ITU iGEM team @ Istanbul</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7061-4"><!-- content -->
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<p>Seminar about synthetic biology given by us to METU College students</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7070-4"><!-- content -->
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<p>Hanging out with Baskent Uni iGEM team</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7079-4"><!-- content -->
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<p>Modelling team is working, not playing LoL.</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7088-4"><!-- content -->
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<p>Participating in Treasure Hunt '13 in METU</p>
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</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7097-4"><!-- content -->
 +
<p>Modelling team saying goodbye to our captain, Buse Reis, who kept in touch with us in Cambridge.</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7106-4"><!-- content -->
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<p>Strengthening our bonds by group activities</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7115-4"><!-- content -->
 +
<p>The collaboration of all iGEM teams in Turkey. We presented our projects to each other and our advisors to improve them and to get feedback.</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7124-4"><!-- content -->
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<p>Making an introduction to lab works for ITU&nbsp; MOBGAM team</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7142-4"><!-- content -->
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<p>Making an introduction to lab works for ITU&nbsp; MOBGAM team</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7151-4"><!-- content -->
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<p>#direnplate</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7160-4"><!-- content -->
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<p>One of our meetings with Assoc. Prof. Dr. Mesut MUYAN</p>
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</div>
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<div class="SSSlideCaption invi clearfix grpelem" id="u7169-4"><!-- content -->
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<p>Collaboration and education with mechanical engineers of METU</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7178-4"><!-- content -->
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<p>Ancient autoclave found in METU Biological Sciences building</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7187-4"><!-- content -->
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<p>Planning the circuits</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7196-4"><!-- content -->
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<p>Hosting future iGEMers from Güven Eğitim &amp; Sağlık Vakfı in METU</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7205-4"><!-- content -->
 +
<p>Young iGEMers appreciating science</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7214-4"><!-- content -->
 +
<p>Our captain after doing&nbsp; hard laboratory work :D</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7223-4"><!-- content -->
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<p>SCIENCE B*TCH</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7241-4"><!-- content -->
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<p>Enjoying our bee cookies aseptically in lab</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7286-4"><!-- content -->
 +
<p>Thanks to Nihal'in Elinden for cookies</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7250-4"><!-- content -->
 +
<p>Enjoying our bee cookies not so very aseptically in lab</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7509-4"><!-- content -->
 +
<p>Falcon quality control :p</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7268-4"><!-- content -->
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<p>Modelling team at work</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7277-4"><!-- content -->
 +
<p>Raising awareness about synthetic biology in METU</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7295-4"><!-- content -->
 +
<p>Brainstorming for the project</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7376-4"><!-- content -->
 +
<p>SynBio Party poster</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7313-4"><!-- content -->
 +
<p>SynBio Party cocktails</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7322-4"><!-- content -->
 +
<p>SynBio Party people having fun</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7331-4"><!-- content -->
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<p>SynBio Party</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7340-4"><!-- content -->
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<p>SynBio Party 300 &#45;500</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7349-4"><!-- content -->
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<p>SynBio Party</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7358-4"><!-- content -->
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<p>SynBio Party :D</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7367-4"><!-- content -->
 +
<p>SynBio Party which made us some funds</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7385-4"><!-- content -->
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<p>Synthetic Biology Day Crew</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7421-4"><!-- content -->
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<p>Our beloved participants in Synthetic Biology day</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7439-4"><!-- content -->
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<p>Synthetic Biology Day Crew and Participants</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7412-4"><!-- content -->
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<p>iGEM Stand in METU</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7394-4"><!-- content -->
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<p>iGEM stand in METU</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7403-4"><!-- content -->
 +
<p>Keep Calm and Follow Us</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7430-4"><!-- content -->
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<p>Hülooooğğğğ</p>
 +
</div>
 +
<div class="SSSlideCaption invi clearfix grpelem" id="u7133-4"><!-- content -->
 +
<p>Safety first :D</p>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<img class="grpelem" id="u5739-4" src="http://ghucuk.netii.net/u5739-4.png" alt="Check Our Gallery" width="218" height="22"/><!-- rasterized frame -->
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<a class="nonblock nontext clearfix colelem" id="u20293-4" href="http://metuigem.com/"><!-- content --><p>Click here for our national site!</p></a>
 +
<a class="nonblock nontext clearfix colelem" id="u20291-4" href="http://blog.metuigem.com/"><!-- content --><p>Click here for our blog!</p></a>
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<a class="nonblock nontext clearfix grpelem" id="u20292-4" href="http://www.flickr.com/photos/igemmetu2013/"><!-- content --><p>Click here for even more photos!</p></a>
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Latest revision as of 03:55, 5 October 2013

Home

Our Project Description:

 Taking a major role in pollination, bees are one of the most important organisms within an ecosystem. However their populations are in serious decline. Colony Collapse Disorder has been found as the most common cause of the disappearance of bees in large numbers. Some pathogens like parasitic fungi and a pesticide called imidacloprid are the leading reasons of CCD. In this study, our aim is to decrease the number of hives affected by chemical compounds such as imidacloprid. Our plan is to turn the mutualistic bacteria living in bees' guts into a shield mechanism to protect the bees against these factors. A protein CYP6G1 found in Drosophilia melanogaster has the ability to degrade imidacloprid into harmless substances. Moreover, coumaric acid increases the general immunity of bees against harmful components and parasitic organisms and we aim to increase the level of coumaric acid in bees' guts. The main objective of this study is the transformation of the genes coding for these two proteins to Bacillus subtilis, which mutualistically live in bees' guts.
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Retrieved from "http://2013.igem.org/Team:METU_Turkey"