Team:Heidelberg/Templates/DelH week25
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(Difference between revisions)
(Created page with "(18.10) ==HM17-FS_78== Annealing temps: 62°C, 60°C, 57°C, 55°C <br/> Elongation time: 2:10 ==Screening remaining Gibson electroporated== 60 colonies were picked and screened ...") |
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60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62) | 60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62) | ||
==Gibson assembly== | ==Gibson assembly== | ||
+ | {|class="wikitable" | ||
+ | |- | ||
+ | ! Reagent !! Volume added (µl) | ||
+ | |- | ||
+ | | BB (FS..) || 0.61 | ||
+ | |- | ||
+ | | DN11-FS66 || 1.00 | ||
+ | |- | ||
+ | | FS_67-FS_68 || 1.35 | ||
+ | |- | ||
+ | | FS_69-FS_70 || 6.34 | ||
+ | |- | ||
+ | | FS_71-HM08 || 0.70 | ||
+ | |- | ||
+ | | Gibson Master Mix 2x || 10 | ||
+ | |} |
Revision as of 15:23, 18 October 2013
(18.10)
HM17-FS_78
Annealing temps: 62°C, 60°C, 57°C, 55°C
Elongation time: 2:10
Screening remaining Gibson electroporated
60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62)
Gibson assembly
Reagent | Volume added (µl) |
---|---|
BB (FS..) | 0.61 |
DN11-FS66 | 1.00 |
FS_67-FS_68 | 1.35 |
FS_69-FS_70 | 6.34 |
FS_71-HM08 | 0.70 |
Gibson Master Mix 2x | 10 |