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| - | This year we aim to solve the problem of quantitative multiple gene regulations on genomic level. To fulfill this goal, we need 2 systems in total. One is a regulating system acting on genomic genes, and the other is a controlling system functioning on regulating part, better with a user-friendly interface.
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| - | After careful discussion and selection, we decide to use the newly devised gene regulating tool named CRISPRi(Clustered Regularly Interspaced Short Palindromic Repeats interference) as a perfect candidate for efficiently knocking down specific genes simultaneously and independently according to different people's need. It has also been proved that this system can act on genomic genes with no detectable off-target effects. Then we choose three light controlled gene expression systems as the controlling part, each of them will control an sgRNA(small guide RNA), a key part of CRISPRi system, to regulate different targeted genes. By changing light intensity, the strength of the promoter will vary, thus realizing quantitative regulation of genes.
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| - | With the combination of these 2 systems, we are able to use common LED light to regulate different genomic genes. Moreover, considering advantages of using light as an induced signal, we have designed a dark box and written a software as our experiment measurements. Finally, we can adjust different genomic genes expression levels just by typing in several numbers.
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| - | Our project demonstrates a wide range of new application areas for both fundamental scientific research and industrial producing process.
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| | =<font color=white>Why CRISPRi?</font>= | | =<font color=white>Why CRISPRi?</font>= |
| - | <font color=white>
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| - | *Regulate any target genes without extra structure design.
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| - | *Totipotent. Change the target gene as you want by changing sgRNA sequence. | + | * Regulate genomic genes without changing their promoters. |
| - | </font> | + | |
| | + | * "Totipotent"! Change the target gene as you want by making some minor changes in gRNA sequence. |
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| | + | =<font color=white>Why Lights?</font>= |
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| | + | * Easy to adjust! Get rid of those laborious work of adding inducing chemicals! :) |
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| - | =<font color=white>Why Light?</font>=
| + | * Multi-control! lights of different colors can be exerted simultaneously. And one sensor will only be activated by photons with a narrow range of wavelengths. |
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| - | * Easy to adjust. Get rid of those laborious work of adding inducing chemicals. | + | * Accurate! |
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| - | *Accurate. | + | * Uniformly distribute in cell culture. |
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| - | * Distribute uniformly when culturing.
| + | =<font color=white>Why CRISPRi AND Lights?</font>= |
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| - | =<font color=white>Why CRISPRi and Light?</font>=
| + | * Lights alone? CANNOT be easily applied on genomic genes. |
| - | *Universal. Regulate multiple genes in one same metabolic pathway simultaneously, independently and quantitatively. | + | |
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| - | *Extensible. The number of genes being regulated can be easily increased if other light sensing systems become available, which will further increase the complexity and accuracy of our regulating system. | + | * CRISPRi alone? HARD to be simultaneous; NOT convenient. |
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| | =<font color=white>Why Computer?</font>= | | =<font color=white>Why Computer?</font>= |
| | Computer and Electronics! Experience the information century! | | Computer and Electronics! Experience the information century! |
| - | * Input your desired expression level into the computer. And just leave the rest to our program and illuminating device to adjust to your specific requirement. | + | * Input your desired expression level into our User Interface on the computer. And just leave rest of the work to our program and our illuminating device! |
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