Latest revision as of 21:12, 18 October 2013

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Overview Project Overview

Luminous System Design Device Control

Light Sensors Tri-light! Red Green Blue

Regulator-CRISPRi Introdution to CRISPRi Why CRISPRi? Integrating CRISPRi

Project Overview

Why CRISPRi?

Regulate genomic genes without changing their promoters.

"Totipotent"! Change the target gene as you want by making some minor changes in gRNA sequence.

Why Lights?

Easy to adjust! Get rid of those laborious work of adding inducing chemicals! :)

Multi-control! lights of different colors can be exerted simultaneously. And one sensor will only be activated by photons with a narrow range of wavelengths.

Accurate!

Uniformly distribute in cell culture.

Why CRISPRi AND Lights?

Lights alone? CANNOT be easily applied on genomic genes.

CRISPRi alone? HARD to be simultaneous; NOT convenient.

Why Computer?

Computer and Electronics! Experience the information century!

Input your desired expression level into our User Interface on the computer. And just leave rest of the work to our program and our illuminating device!

@@ Line 39: / Line 39: @@
 =<font color=white>Why CRISPRi?</font>=
-*Regulate genomic genes without changing their promoters.
+* Regulate genomic genes without changing their promoters.
-*"Totipotent". Change the target gene as you want by making some minor changes in gRNA sequence.
+* "Totipotent"! Change the target gene as you want by making some minor changes in gRNA sequence.
-=<font color=white>Why Light?</font>=
+=<font color=white>Why Lights?</font>=
-* Easy to adjust. Get rid of those laborious work of adding inducing chemicals.
+* Easy to adjust! Get rid of those laborious work of adding inducing chemicals! :)
-* Accurate.
+* Multi-control! lights of different colors can be exerted simultaneously. And one sensor will only be activated by photons with a narrow range of wavelengths.
-* Distribute uniformly when culturing.
+* Accurate!
-=<font color=white>Why CRISPRi AND Light?</font>=
+* Uniformly distribute in cell culture.
-*Universal. Regulate multiple genes in one same metabolic pathway simultaneously, independently and quantitatively.
-*Extensible. The number of genes being regulated can be easily increased if other light sensing systems become available, which will further increase the complexity and accuracy of our regulating system.
+=<font color=white>Why CRISPRi AND Lights?</font>=
+* Lights alone? CANNOT be easily applied on genomic genes.
+* CRISPRi alone? HARD to be simultaneous; NOT convenient.
 =<font color=white>Why Computer?</font>=
 Computer and Electronics! Experience the information century!
-* Input your desired expression level into the computer. And just leave the rest to our program and illuminating device to adjust to your specific requirement.
+* Input your desired expression level into our User Interface on the computer. And just leave rest of the work to our program and our illuminating device!
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Team:SJTU-BioX-Shanghai/Project

From 2013.igem.org