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Exeter/7 July 2013
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Fentwistle (Talk | contribs) (Created page with "'''Making standard liquid cultures of our transformed cells''' In each 10ml Falcon, we need... - 5ml LB broth - 5ul antibiotic (in this case, all of our transformed cells are ...") |
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'''Making standard liquid cultures of our transformed cells''' | '''Making standard liquid cultures of our transformed cells''' | ||
| - | In each | + | In each 10 ml Falcon, we need... |
| - | - | + | - 5 ml LB broth |
| - | - | + | - 5 ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone) |
| - | We used a | + | We used a 50 ml conical flask of LB broth which had been autoclaved and added 50 ul of chloramphenicol to make a "stock" to be pipetted into the Falcons. |
Stab the chosen colony with a pipette tip and eject into the Falcon tube. | Stab the chosen colony with a pipette tip and eject into the Falcon tube. | ||
The tubes are incubated overnight in a spinning 37oC incubator. | The tubes are incubated overnight in a spinning 37oC incubator. | ||
Revision as of 10:44, 16 July 2013
Making standard liquid cultures of our transformed cells
In each 10 ml Falcon, we need...
- 5 ml LB broth
- 5 ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)
We used a 50 ml conical flask of LB broth which had been autoclaved and added 50 ul of chloramphenicol to make a "stock" to be pipetted into the Falcons.
Stab the chosen colony with a pipette tip and eject into the Falcon tube.
The tubes are incubated overnight in a spinning 37oC incubator.
