Team:Heidelberg/Templates/Indigoidine week7 overview

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===Indigoidine Production - bpsA===
===Indigoidine Production - bpsA===
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Since we couldn't observe indigoidine production using [[Plasmids#pMM64|pMM64]] and [[Plasmids#pMM65|pMM65]] in the previous two weeks we validated the pMM-plasmids using restriction digests.
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Since we couldn't observe indigoidine production using pMM64 and pMM65 in the previous two weeks we validated the pMM-plasmids using restriction digests.
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The restriction digest of [[Plasmids#pMM65|pMM65]] was correct but [[Plasmids#pMM64|pMM64]] didn't show the expected bands on an agarose gel. We continued trying to amplify the bpsA and svp gene in a PCR screening. </p>
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The restriction digest of pMM65 was correct but pMM64 didn't show the expected bands on an agarose gel. We continued trying to amplify the bpsA and svp gene in a PCR screening. </p>

Latest revision as of 15:06, 21 October 2013

Indigoidine Production - bpsA

Since we couldn't observe indigoidine production using pMM64 and pMM65 in the previous two weeks we validated the pMM-plasmids using restriction digests.

The restriction digest of pMM65 was correct but pMM64 didn't show the expected bands on an agarose gel. We continued trying to amplify the bpsA and svp gene in a PCR screening.

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