Team:Heidelberg/Templates/DelH week24

From 2013.igem.org

(Difference between revisions)
(Created page with " == 23-09 - 29-09-13 == ===Generation of DelH plasmids pFS02, pFS03, pFS04 and pFS05=== ====Amplification of pFS_04 Backbone (FS_85 to FS_94; 4.1 kbp)==== :'''Reaction''' {| cla...")
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== 23-09 - 29-09-13 ==
== 23-09 - 29-09-13 ==
===Generation of DelH plasmids pFS02, pFS03, pFS04 and pFS05===
===Generation of DelH plasmids pFS02, pFS03, pFS04 and pFS05===
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! Reagent !! Amount   
! Reagent !! Amount   
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|-
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| ''D. acidovorans'' SPH-1 colony || 1
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| pSB6A1 || 1
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|-
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| SR_01:  (1/10) || 2
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| FS_85:  (1/10) || 1
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|-
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| FS_23:  (1/10) || 2
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| FS_94:  (1/10) || 1
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|-
| Phusion flash Master Mix || 10
| Phusion flash Master Mix || 10
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| 1 || 98 || 10
| 1 || 98 || 10
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| rowspan="3"| 12 || 98 || 1
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| rowspan="2"| 35 || 98 || 1
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| 66 ↓ 0.5  || 5
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| 72  || 3:20
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| rowspan="2"| 18 || 98 || 1
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| 64  || 5
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| 72  || 3:20
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| 72  || 1:25
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| 1 || 72 || 10min
| 1 || 72 || 10min

Revision as of 15:03, 22 October 2013

23-09 - 29-09-13

Generation of DelH plasmids pFS02, pFS03, pFS04 and pFS05

Amplification of pFS_04 Backbone (FS_85 to FS_94; 4.1 kbp)

Reaction
Reagent Amount
pSB6A1 1
FS_85: (1/10) 1
FS_94: (1/10) 1
Phusion flash Master Mix 10
DMSO 1
dd H2O 4
Conditions
Biorad T100
Cycles temperature [°C] Time [s]
1 98 10
35 98 1
72 1:25
1 72 10min
1 12 inf

Results:

Retrieved from "http://2013.igem.org/Team:Heidelberg/Templates/DelH_week24"