The major goals of our modeling work are: validating our project design, simulating its working condition and fitting our simulation with our wet-lab result. The genetic pathway we used is described as below.

The enzymes that can induce the expression of propanoic acid is replaced with mRFP. Throughout the whole modelling work, we choose to study AraC instead of mRFP since they are in the same plasmid and we assumed that the expression rate of both protein is similar. By doing this, we can reduce the number of equations. We divide our modelling work into three parts:
(1)Feasibility of genetic oscillator and its proper parameters.
(2)Oscillation of a group of genetic oscillator cells.
(3)Fitting our experiment data with our simulation data.
To be more specifically, we want to find out if this genetic oscillator can oscillate in the first place, if its period can be adjusted, if it is stable against environment changes, whether a large group of these oscillators can oscillate as well and how our our modelling result fit with our wet-lab result.

If you want to learn about details, please click HERE to download a 11 pages thesis, including every aspects of our work.

You may also want to download a supplementary data of our thesis. Formulas that cannot put on website due to space limitation and raw data are included.