Team:BIT/project hardware

From 2013.igem.org

(Difference between revisions)
Line 159: Line 159:
width: 824px;
width: 824px;
margin-left: 200px;
margin-left: 200px;
-
overflow-x:hidden;
 
-
overflow-y:auto;
 
}
}
#right a {color: #09F;}
#right a {color: #09F;}
Line 190: Line 188:
padding-bottom: 20px;
padding-bottom: 20px;
padding-left: 20px;
padding-left: 20px;
-
}
 
-
.t3{ text-align:center; }
 
-
.t4{
 
-
font-family: Verdana, Geneva, sans-serif;
 
-
font-size: medium;
 
-
color: #CFF;
 
-
line-height: 25px;
 
-
padding-top: 10px;
 
-
padding-right: 20px;
 
-
padding-bottom: 20px;
 
-
padding-left: 20px;
 
-
        text-align:center;
 
}
}
.bg{ background-color:black;}
.bg{ background-color:black;}
Line 215: Line 201:
         <tr>
         <tr>
           <td width="713"><div><a id="a1" href="https://2013.igem.org/wiki/index.php?title=Team:BIT/project_hardware&action=edit"></a></div></td>
           <td width="713"><div><a id="a1" href="https://2013.igem.org/wiki/index.php?title=Team:BIT/project_hardware&action=edit"></a></div></td>
-
           <td width="167"><div><a id="a2" href="http://www.igem.org/Main_Page"></a></div></td>
+
           <td width="167"><div><a id="a2" href="https://2013.igem.org/Main_Page"></a></div></td>
           <td width="130"><div><a id="a3" href="https://2013.igem.org/wiki/index.php?title=Special:UserLogout&returnto=Main_Page"></a></div></td>
           <td width="130"><div><a id="a3" href="https://2013.igem.org/wiki/index.php?title=Special:UserLogout&returnto=Main_Page"></a></div></td>
         </tr>
         </tr>
Line 234: Line 220:
</div>
</div>
-
<div id="class">
+
-
  <table width="1024" border="0" class="bg">
+
      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;As we know, antibiotics are widely used in modern industry to prevent the infection of cow breasts. However, the residual of antibiotics and other components, such as Cr (VI) which is widely used in the recycle of proteins from leather, will endanger the health of customers. Therefore, it is necessary to find solution to identify these harmful chemicals, but the method of timely detection of harmful chemicals in products still remains unsolved. <br/>
-
        <tr>
+
  &nbsp;&nbsp;&nbsp;&nbsp;To solve this problem, our sensor has been divided into three, respectively, of <a id="b1" href="https://2013.igem.org/Team:BIT/project_biosensors">hexavalent chromium, tetracycline and β-lactam</a> there is a response, for each sensor, we are all assembled in a downstream of the <a id="b1" href="https://2013.igem.org/Team:BIT/project_AC">amplification block</a> (amplifier) and the <a  id="b1" href="https://2013.igem.org/Team:BIT/project_AC">control block </a>(controller), the output signal to be amplified, by adjusting the concentration of IPTG to achieve the adjustment of magnification. Amplifier by the T7 RNA polymerase gene and T7 promoter composition control block is through the downstream of the T7 promoter plus <i>lacO</i> and the addition of regular expression <i>lacI</i>, by adjusting the concentration of IPTG, <i>lacO</i> then adjust the boot situation achieved, the final response module for the green fluorescent protein.<br/>
-
          <td><div id="c1"><ul><li><a href="https://2013.igem.org/Team:BIT/project_AC">>>MORE</a></li></ul></div></td>
+
  &nbsp;&nbsp;&nbsp;&nbsp;In order to achieve real-time detection, we also made a <a  id="b1" href="https://2013.igem.org/Team:BIT/project_microchip">biochip</a> which provided a reaction room for both engineering bacteria and samples. Now we are able to detect all these three harmful chemicals with a<a id="b1" href="https://2013.igem.org/Team:BIT/project_hardware"> hand-held electronic equipment</a> made by ourselves.</td>
-
          <td><div id="c2"><ul><li><a href="https://2013.igem.org/Team:BIT/project_biosensors">>>MORE</a></li></ul></div></td>
+
-
          <td><div id="c3"><ul><li><a href="https://2013.igem.org/Team:BIT/project_microchip">>>MORE</a></li></ul></div></td>
+
-
          <td><div id="c4"><ul><li><a href="https://2013.igem.org/Team:BIT/project_hardware">>>MORE</a></li></ul></div></td>
+
-
        </tr>
+
-
</table>
+
-
</div>
+
-
<div id="main">
+
-
  <div id="left">
+
-
    <ul>
+
-
<li><a href="https://2013.igem.org/Team:BIT">HOME</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BIT/project_biosensors">BIOSENSORS</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BIT/project_AC">AMPLIFIER</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BIT/project_microchip">MICRO CHIP</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BIT/project_hardware">HARDWARE</a></li>
+
-
</ul>
+
-
    </div>
+
-
<div id="right">
+
-
  <table width="824" border="0" class="bg3">
+
-
    <tr>
+
-
      <td width="608" class="t1">Hardware</td>
+
-
        </tr>
+
-
<tr>
+
-
      <td class="t2"><strong>Introduction</strong></td>
+
-
        </tr>
+
-
<tr>
+
-
            <td class="t3"><img src="https://static.igem.org/mediawiki/2013/2/2b/BIT_H1.jpg" alt="" width="355" height="250">
+
-
            </td>
+
-
</tr>
+
-
<tr>
+
-
            <td class="t3"><img src="https://static.igem.org/mediawiki/2013/0/06/BIT_H2.png" alt="" width="736" height="299">
+
-
            </td>
+
-
</tr>
+
-
<tr>
+
-
      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;As the joint of biological and non-biological research, this device aims to detect the fluorescent intensity of GFP and calculate the concentration of the chemicals (Cr (VI), beta-lactam, and tetracycline, respectively) detected based on it. We can then assess the quality of the sample detected.
+
-
</td>
+
-
        </tr>
+
-
<tr>
+
-
            <td class="t3"><img src="https://static.igem.org/mediawiki/2013/0/00/BIT_H3.png" alt="" width="450" height="350">
+
-
            </td>
+
-
</tr>
+
-
<tr>
+
-
    <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp; The mechanism of this device is simple (figure). The exciting light coming from blue LED through 490nm narrowband filter, which only allow 490nm light  to pass through. The filtered light then penetrates our test chip. If GFP exists on our test chip, it would transform the frequency of the excitation light into about 520nm, which is the only frequency of light that can pass through the 517nm narrowband filter. Then our sensor will be able to detect the intensity of the light and calculate the content of GFP, indicating the composition of the tested sample with our mathematical model.<br>
+
-
          &nbsp;&nbsp;&nbsp;&nbsp; The characteristic of our device is that it is really CHEAP. Although it costs less than 300RMB in total and can easily cooperate with our biological products.
+
-
</td>
+
-
</tr>
+
-
<tr>
+
-
      <td class="t2"><strong>Result</br></br>
+
-
A) Linear Regression</strong></td>
+
-
</tr>
+
-
      <tr>
+
-
    <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp; To indicate the reliability of our system, we conducted experiments with fluorescein sodium solutions of different concentration. The figure below shows the curve of our result, which fits perfectly with the linear curve with an R-squared of 0.9842. The result indicates that our detection system is reliable.</td>
+
-
</tr>
+
-
<tr>
+
-
            <td class="t3"><img src="https://static.igem.org/mediawiki/2013/5/52/BIT_H_R1.png">
+
-
            </td>
+
-
</tr>
+
-
<tr>
+
-
<td class="t2"><strong>B) Blind Test</strong></td>
+
-
</tr>
+
-
<tr>
 
-
    <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp; We conducted a “Blind test”, in which we detected the concentration of antibiotics in pure water. In the test, we had seven samples:</br>
 
-
1) Five SAFE samples, with only pure water;</br>
 
-
2) One HARMFUL sample, with a low concentration of antibiotics;</br>
 
-
3) One TOXIC sample, with a high concentration of antibiotics.</br>
 
-
Before the test, we did not know which sample was safe and which sample was harmful. Both of the conventional method (VarioskaFlash) and our new method were adopted to detect the concentration of antibiotics in the sample and here is the result. As can be seen from the figure below, the data of our detector was consistent with that of VarioskaFlash, which means our system really worked well.
 
-
</td>
 
-
</tr>
 
-
<tr>
 
-
            <td class="t3"><img src="https://static.igem.org/mediawiki/2013/3/30/BIT_H_R2.png"><br><br>
 
-
            </td>
 
-
</tr>
 
-
 
-
 
-
 
-
 
-
      </table>
 
-
</div>
 
-
</div>
 
-
<div id="footer1">
 
-
  <table width="1024" border="0" class="bg2">
 
-
      <tr>
 
-
        <td>&nbsp;</td>
 
-
        <td>&nbsp;</td>
 
-
        <td>&nbsp;</td>
 
-
      </tr>
 
-
      <tr>
 
-
        <td width="110">&nbsp;</td>
 
-
        <td width="790">Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081 </td>
 
-
        <td width="110">&nbsp;</td>
 
-
      </tr>
 
-
      <tr>
 
-
        <td>&nbsp;</td>
 
-
        <td> E-mail: yifei0114@bit.edu.cn</td>
 
-
        <td>&nbsp;</td>
 
-
      </tr>
 
-
      <tr>
 
-
        <td>&nbsp;</td>
 
-
        <td>Beijing Institute of Technology © 2013<a id="d1" href="http://english.bit.edu.cn/">&nbsp;Privacy Policy</a></td>
 
-
        <td>&nbsp;</td>
 
-
      </tr>
 
-
  </table>
 
-
</div>
 
</body>
</body>
</html>
</html>

Revision as of 11:53, 26 October 2013

iGEM BIT

    As we know, antibiotics are widely used in modern industry to prevent the infection of cow breasts. However, the residual of antibiotics and other components, such as Cr (VI) which is widely used in the recycle of proteins from leather, will endanger the health of customers. Therefore, it is necessary to find solution to identify these harmful chemicals, but the method of timely detection of harmful chemicals in products still remains unsolved.
    To solve this problem, our sensor has been divided into three, respectively, of hexavalent chromium, tetracycline and β-lactam there is a response, for each sensor, we are all assembled in a downstream of the amplification block (amplifier) and the control block (controller), the output signal to be amplified, by adjusting the concentration of IPTG to achieve the adjustment of magnification. Amplifier by the T7 RNA polymerase gene and T7 promoter composition control block is through the downstream of the T7 promoter plus lacO and the addition of regular expression lacI, by adjusting the concentration of IPTG, lacO then adjust the boot situation achieved, the final response module for the green fluorescent protein.
    In order to achieve real-time detection, we also made a biochip which provided a reaction room for both engineering bacteria and samples. Now we are able to detect all these three harmful chemicals with a hand-held electronic equipment made by ourselves.

Retrieved from "http://2013.igem.org/Team:BIT/project_hardware"