Team:XMU Software/html

From 2013.igem.org

(Difference between revisions)

Latest revision as of 05:18, 27 October 2013

18A-AI-2M19

2013/7/29 09:15:49--Ruosang

2013/7/29 09:16:24

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/7/30 09:18:19

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/7/30 09:19:34

2013/7/30 09:21:18

EX SP
Extract plasmid
	(A)	(B)	(C)
Plasmid

EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H₂0 /μL

2013/7/30 09:21:31

2013/7/30 09:22:23

Analyse Electrophoretogram
Electrophoretogram and recover
	A_260/280	ng/μL	bp
(A)
(B)
(C)
Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel

2013/7/30 09:23:12

Ligation

	Recommend	Actual use
A (vector) /μL
B (insert) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/7/31 09:23:55

2013/8/1 09:24:26--Ruosang

2013/8/1 09:24:42

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/1 09:25:15

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/1 09:25:39

2013/8/5 21:44:19--Ruosang

2013/8/5 21:45:25

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/5 21:45:55

2013/8/6 21:46:26

2013/8/7 21:50:17

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/7 21:50:56

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/7 21:52:12

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/7 21:52:52

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/8 21:54:37

2013/8/9 22:05:07

2013/8/9 22:13:49

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/9 22:14:05

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/16 23:58:07

2013/8/7 21:46:54--Ruosang

2013/8/7 21:47:35

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/7 21:48:21

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/7 21:48:47

2013/8/9 22:02:31

2013/8/9 22:15:05

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/9 22:15:18

Cultivation

Plasmid (A )

Strain

antibiotics

Medium

Volume

Temperature

℃

Speed

Plasmid (B )

Strain

antibiotics

Medium

Volume

Temperature

℃

Speed

2013/8/10 22:16:21

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/16 23:58:45

2013/8/8 21:56:45--Ruosang

2013/8/8 21:57:12

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/8 22:08:20

2013/8/9 22:09:20--Ruosang

2013/8/9 22:09:38

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/9 22:10:21

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/9 22:11:10

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/9 22:11:57

2013/8/12 22:22:06--Ruosang

2013/8/12 22:22:24

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/12 22:22:43

Cultivation

Plasmid (A )

Strain

antibiotics

Medium

Volume

Temperature

℃

Speed

Plasmid (B )

Strain

antibiotics

Medium

Volume

Temperature

℃

Speed

2013/8/13 23:21:13

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/13 23:21:47

2013/8/13 23:25:17

EX SP
Extract plasmid
	(A)	(B)	(C)
Plasmid

EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H₂0 /μL

2013/8/13 23:36:12

2013/8/15 23:41:26--Ruosang

2013/8/15 23:41:42

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/15 23:44:36

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/15 23:43:57

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/15 23:47:13

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/15 23:52:24

EX SP
Extract plasmid
	(A)	(B)	(C)
Plasmid

EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H₂0 /μL

2013/8/15 23:54:43

2013/8/15 23:55:52

Analyse Electrophoretogram
Electrophoretogram and recover
	A_260/280	ng/μL	bp
(A)
(B)
(C)
Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel

2013/8/15 23:56:29

Ligation

	Recommend	Actual use
A (insert) /μL
B (vector) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/16 23:59:03

2013/8/17 23:59:37

2013/8/19 00:07:27

2013/8/19 00:09:02

Ligation

	Recommend	Actual use
A (insert) /μL
B (vector) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/20 00:13:32

2013/8/20 00:14:31--Ruosang

2013/8/20 00:14:48

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/20 00:15:21

2013/8/20 00:16:34

2013/8/20 00:16:50

Ligation

	Recommend	Actual use
A (vector) /μL
B (insert) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/21 00:17:26--Ruosang

2013/8/21 00:17:45

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/21 00:18:04

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/22 00:18:33--Ruosang

2013/8/22 00:18:50

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/22 00:19:07

2013/8/22 00:20:05

EX SP
Extract plasmid
	(A)	(B)	(C)
Plasmid

EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H₂0 /μL

2013/8/22 00:20:33

Ligation

	Recommend	Actual use
A (vector) /μL
B (insert) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/23 00:21:17--Ruosang

2013/8/23 00:21:30

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/23 00:21:48

2013/8/23 00:23:10--Ruosang

2013/8/23 00:23:21

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (C)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/23 00:24:04

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

(C)

2013/8/23 00:24:54

2013/8/23 00:25:28

2013/8/23 00:26:59--Ruosang

2013/8/23 00:27:17

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/23 00:28:07

EX SP
Extract plasmid
	(A)	(B)	(C)
Plasmid

EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H₂0 /μL

2013/8/23 00:29:20

Analyse Electrophoretogram
Electrophoretogram and recover
	A_260/280	ng/μL	bp
(A)
(B)
(C)
Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel

2013/8/23 00:29:43

Ligation

	Recommend	Actual use
A (vector) /μL
B (insert) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/24 00:33:05

2013/8/25 00:30:23

Ligation

	Recommend	Actual use
A (vector) /μL
B (insert) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/25 00:30:39

2013/8/26 00:33:48

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/26 00:34:19

PCR-Reaction
Reaction /μL

dNTP ( Mixture（2.5 mM each） ) /μL


Template /μL
rTaq /μL
H₂O /μL

2013/8/26 00:31:30

2013/8/28 12:02:00

2013/8/29 11:28:51--Ruosang

2013/8/29 11:29:30

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/29 11:30:51

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/29 11:32:21

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/29 11:34:47

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/29 11:41:40

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/29 11:42:42

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/29 11:43:26

2013/8/29 11:44:18

2013/8/31 11:44:58

2013/9/2 11:52:55

pBAD-2M19

2013/8/3 18:01:27--Ruosang

2013/8/3 18:01:51

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/3 18:02:03

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/3 18:03:36

EX SP
Extract plasmid
	(A)	(B)	(C)
Plasmid

EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H₂0 /μL

2013/8/3 18:03:49

2013/8/3 18:04:55--Ruosang

2013/8/3 18:05:49

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/3 18:08:04

Plasmid Extraction

A_260/280

ng/μL

(A)

(B)

2013/8/3 18:10:12--Ruosang

2013/8/3 18:10:41

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/3 18:11:50

EX SP
Extract plasmid
	(A)	(B)	(C)
Plasmid

EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H₂0 /μL

2013/8/3 19:45:37

2013/8/3 18:13:13

Analyse Electrophoretogram
Electrophoretogram and recover
	A_260/280	ng/μL	bp
(A)
(B)
(C)
Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel

2013/8/3 18:13:35

Ligation

	Recommend	Actual use
A (vector) /μL
B (insert) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/3 18:14:18

2013/8/5 11:54:23

Ligation

	Recommend	Actual use
A (vector) /μL
B (insert) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/8 12:43:18

2013/8/9 12:49:26

2013/8/10 22:17:02--Ruosang

2013/8/10 22:17:31

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/8/10 22:17:54

EX SP
Extract plasmid
	(A)	(B)	(C)
Plasmid

EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H₂0 /μL

2013/8/10 22:18:10

2013/8/10 22:18:46

Analyse Electrophoretogram
Electrophoretogram and recover
	A_260/280	ng/μL	bp
(A)
(B)
(C)
Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel

2013/8/10 22:19:07

Ligation

	Recommend	Actual use
A (insert) /μL
B (vector) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/10 22:19:36

Ligation

	Recommend	Actual use
A (insert) /μL
B (vector) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/8/10 22:20:43

2013/8/13 23:24:12--Ruosang

2013/8/13 23:25:39

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/13 23:28:09

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/13 23:28:51

2013/8/18 00:00:42--Ruosang

2013/8/18 00:01:15

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/18 00:02:21

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/18 00:02:55

PCR-Reaction
Reaction /μL

dNTP ( Mixture（2.5 mM each） ) /μL


Template /μL
rTaq /μL
H₂O /μL

2013/8/18 00:05:05

2013/8/20 00:11:54

PCR-Reaction
Reaction /μL

dNTP ( Mixture（2.5 mM each） ) /μL


Template /μL
rTaq /μL
H₂O /μL

2013/8/20 00:10:09

2013/8/31 11:49:18

2013/8/26 00:35:21--Ruosang

2013/8/26 00:35:54

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/26 00:37:04

PCR-Reaction
Reaction /μL

dNTP ( Mixture（2.5 mM each） ) /μL


Template /μL
rTaq /μL
H₂O /μL

2013/8/26 00:40:19

2013/8/27 00:42:13

2013/8/27 00:42:54

2013/8/28 11:26:54

2013/9/16 12:56:58

2013/9/16 12:58:29

2013/8/26 00:39:17--Ruosang

2013/8/26 00:39:25

Template and Primers
choose plasmid		primer1	name
plasmid	name		sequence
plasmid	location		direction
type			position
part-only	sequence	primer2	name
part-only	length		sequence
backbone	name		direction
backbone	length		position

2013/8/26 00:40:04

PCR-Reaction
Reaction /μL

dNTP ( Mixture（2.5 mM each） ) /μL


Template /μL
rTaq /μL
H₂O /μL

2013/8/26 00:40:51

2013/8/27 00:42:25

2013/8/28 11:27:57

2013/9/6 12:40:40--Ruosang

2013/9/6 12:41:24

Start to Ligate Plasmids

choose plasmid (A)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

choose plasmid (B)
plasmid	name
plasmid	location
type
part-only	sequence
part-only	length
backbone	name
backbone	length

2013/9/6 12:42:40

2013/9/6 12:43:17

2013/9/6 12:44:27

Analyse Electrophoretogram
Electrophoretogram and recover
	A_260/280	ng/μL	bp
(A)
(B)
(C)
Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel

2013/9/6 12:44:44

Ligation

	Recommend	Actual use
A (vector) /μL
B (insert) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/9/7 12:48:34

2013/9/7 12:49:01

Ligation

	Recommend	Actual use
A (vector) /μL
B (insert) /μL

ligase /μL
	Recommend	Actual use
A /μL
B /μL
C /μL

ligase /μL
H₂O /μL

2013/9/7 12:49:19

2013/9/9 12:50:45

@@ Line 4: / Line 4: @@
 		<script src="http://ajax.googleapis.com/ajax/libs/jquery/1.8.0/jquery.min.js"></script>
 		<script type="text/javascript" async="" src="https://2013.igem.org/wiki/index.php?title=Team:XMU_Software/js&action=raw&amp;ctype=text/javascript"></script>
-		<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:XMU_Software/css&action=raw&amp;ctype=text/css"/>
+		<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:XMU_Software/Notebook/css&action=raw&amp;ctype=text/css"/>
+<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:XMU_Software/css&action=raw&amp;ctype=text/css"/>
 	</head>
 <body>
@@ Line 13: / Line 14: @@
 A-AI-2M19
 		</div>
-		<div class="dataOutput">
+		<div class="dataOutput1">
 				<div class="M1_exp_stepInfo" title="AI-2M19" id="sb0s0">			<button class="M1_exp_step_name_button"><input value="AI-2M19" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/7/29 09:15:49--Ruosang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s0i0">2013/7/29 09:16:24</td>			<td class="M1_exp_item_content">			<table id="i0s0i0" class="C8">				<tbody><tr>					<th colspan="2" class="C8_td_title">						Start to Ligate Plasmids					</th>				</tr>				<tr>					<td>						<table class="C8">							<tbody><tr>								<th colspan="3" class="C8_td_title">									choose plasmid (A)				</th>							</tr>							<tr>								<th rowspan="2" class="C3_td">									plasmid								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="AI" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									location								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									type								</th>								<td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									part-only								</th>								<th class="C3_td">									sequence								</th>								<td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="904" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									backbone								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="psb1a2" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="2079" class="C3_input"></td>							</tr>						</tbody></table>					</td>					<td>						<table class="C8">							<tbody><tr>								<th colspan="3" class="C8_td_title">									choose plasmid (B)				</th>							</tr>							<tr>								<th rowspan="2" class="C3_td">									plasmid								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="2M19" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									location								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									type								</th>								<td class="C3_td" colspan="2"><input value="rbs-gfp-tt" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									part-only								</th>								<th class="C3_td">									sequence								</th>								<td class="C3_td"><input value="atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="857" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									backbone								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="psb1a2" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="2079" class="C3_input"></td>							</tr>						</tbody></table>					</td>					<td style="display: none;">						<table class="C8">							<tbody><tr>								<th colspan="3" class="C8_td_title">									choose plasmid (C)				</th>							</tr>							<tr>								<th rowspan="2" class="C3_td">									plasmid								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									location								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									type								</th>								<td class="C3_td" colspan="2"><input value="" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									part-only								</th>								<th class="C3_td">									sequence								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									backbone								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>						</tbody></table>					</td>				</tr>				<tr>					<td class="C3_td" colspan="2">						<input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s0i0">						<input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s0i0" style="display: none;">					</td>				</tr>			</tbody></table></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s0i1">2013/7/30 09:18:19</td>			<td class="M1_exp_item_content">				<table id="i0s0i1" class="C4">					<tbody><tr>						<th colspan="3">Plasmid Extraction</th>						<td rowspan="9" class="C4_td_textarea">							<textarea class="C3_textarea" id="i0s0i19">						</textarea></td>					</tr>					<tr>						<td class="C3_td">      </td>						<th class="C3_td">A<sub>260/280</sub></th>						<th class="C3_td">ng/μL</th>					</tr>					<tr>						<td class="C3_td" align="center">(A)</td>						<td class="C3_td"><input value="1.80" class="C3_input"></td>						<td class="C3_td"><input value="46.4" class="C3_input"></td>					</tr>					<tr>						<td class="C3_td" align="center">(B)</td>						<td class="C3_td"><input value="1.83" class="C3_input"></td>						<td class="C3_td"><input value="26.2" class="C3_input"></td>					</tr>					<tr class="C4_tr_C" style="display: none;">						<td class="C3_td" align="center">(C)</td>						<td class="C3_td"><input value="" class="C3_input"></td>						<td class="C3_td"><input value="" class="C3_input"></td>					</tr>					<tr>						<th class="C3_td" colspan="3">							<button class="C8_button" id="i0s0i112" name="#i0s0i1">								Evaluate							</button>						</th>					</tr>					<tr style="display: none;">						<td colspan="3">							<table class="C3">								<tbody><tr>									<td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td>									<td class="C3_td">great</td>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>								<tr>									<td class="C3_td"><input value="" class="C3_input"></td>									<td class="C3_td">fine</td>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>								<tr>									<td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td>									<td class="C3_td" rowspan="2">not good</td>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>								<tr>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>							</tbody></table>						</td>					</tr>				</tbody></table></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s0i2">2013/7/30 09:19:34</td>			<td class="M1_exp_item_content">	<table id="i0s0i2" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i0s0i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s0i22M19,18AI.jpg"><br>				<form id="i0s0i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i0s0i22" form="i0s0i24" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t1" form="i0s0i24">				<input name="id" type="hidden" value="i0s0i2" form="i0s0i24">				<input name="oldgraph" type="hidden" id="i0s0i25" form="i0s0i24" value="t1i0s0i22M19,18AI.jpg">				<input id="i0s0i26" type="submit" value="submit" form="i0s0i24" class="C1_button2" disabled="">				<div id="i0s0i23" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i0s0i21" class="C1_text">2m19  ai  M500</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s0i3">2013/7/30 09:21:18</td>			<td class="M1_exp_item_content">		<table class="C5" id="i0s0i3">			<tbody><tr>				<th colspan="3" id="i0s0i329" class="C5_th_C">Extract plasmid</th>				<td rowspan="10" class="C3_td_textarea">					<textarea class="C3_textarea" id="i0s0i30">					</textarea>				</td>			</tr>			<tr>				<th class="C5_canvas_td" colspan="3" id="i0s0i330">					<div class="C5_canvas_matrix">					</div>					<div class="C5_TEXT1">EX</div>					<div class="C5_TEXT2">SP</div>					<input type="button" class="C5_canvas_button1" id="i0s0i31" name="#i0s0i3" value="AI (A)">					<input type="button" class="C5_canvas_button2" id="i0s0i32" name="#i0s0i3" value="2M19 (B)">					<input type="button" class="C5_canvas_button3" id="i0s0i33" name="#i0s0i3" value="AI (A)">				</th>			</tr>				<tr>					<th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th>					<th class="C3_td">(A)</th>					<th class="C3_td">(B)</th>					<th class="C3_td_C5_C" style="display: none;">(C)</th>				</tr>				<tr>					<th class="C3_td">Plasmid</th>					<td class="C3_td"><input value="50" class="C3_input"></td>					<td class="C3_td"><input value="50" class="C3_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td>				</tr>				<tr>					<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>					<td class="C3_td"><input value="10" class="C3_input"></td>					<td class="C3_td"><input value="10" class="C3_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td>				</tr>				<tr>					<th class="C3_td"><em>Eco</em>R I /μL</th>					<td class="C3_td"><input value="0" class="C5_input"></td>					<td class="C3_td"><input value="0" class="C5_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td>				</tr>				<tr>					<th class="C3_td"><em>Xba</em> I /μL</th>					<td class="C3_td"><input value="0" class="C5_input"></td>					<td class="C3_td"><input value="5" class="C5_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td>				</tr>				<tr>					<th class="C3_td"><em>Spe</em> I /μL</th>					<td class="C3_td"><input value="5" class="C5_input"></td>					<td class="C3_td"><input value="0" class="C5_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td>				</tr>				<tr>					<th class="C3_td"><em>Pst</em> I /μL</th>					<td class="C3_td"><input value="5" class="C5_input"></td>					<td class="C3_td"><input value="5" class="C5_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td>				</tr>				<tr>					<th class="C3_td">H<sub>2</sub>0 /μL</th>					<td class="C3_td"><input value="30" class="C3_input"></td>					<td class="C3_td"><input value="30" class="C3_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td>					<input type="hidden" id="i0s0i326" value="0">					<input type="hidden" id="i0s0i327" value="1">					<input type="hidden" id="i0s0i328" value="0">				</tr>		</tbody></table></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s0i4">2013/7/30 09:21:31</td>			<td class="M1_exp_item_content">	<table id="i0s0i4" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i0s0i40" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s0i4回收2m19,18ai.jpg"><br>				<form id="i0s0i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i0s0i42" form="i0s0i44" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t1" form="i0s0i44">				<input name="id" type="hidden" value="i0s0i4" form="i0s0i44">				<input name="oldgraph" type="hidden" id="i0s0i45" form="i0s0i44" value="t1i0s0i4回收2m19,18ai.jpg">				<input id="i0s0i46" type="submit" value="submit" form="i0s0i44" class="C1_button2" disabled="">				<div id="i0s0i43" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i0s0i41" class="C1_text">2M19  M500 AI</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s0i5">2013/7/30 09:22:23</td>			<td class="M1_exp_item_content">			<table id="i0s0i5" class="C6">				<tbody><tr>					<th class="C3_td_title" colspan="5">Electrophoretogram and recover</th>					<td rowspan="10" class="C6_td_textarea">						<textarea class="C3_textarea" id="i0s0i56">						</textarea>					</td>				</tr>				<tr>					<th colspan="5" class="C3_td">						Analyse Electrophoretogram					</th>				</tr>				<tr class="mdoelC5Correct0s0i5"> 					<td class="C3_td"></td>					<th class="C3_td">A<sub>260/280</sub></th>					<th class="C3_td">ng/μL</th>					<th class="C3_td">bp</th>					<td class="C3_td" rowspan="4">						<input type="button" value="Correct" class="C6_button" name="0s0i5">					</td>				</tr>				<tr class="mdoelC5Correct0s0i5">					<td class="C3_td" nowrap="nowrap">(A)</td>					<td class="C3_td"><input value="1.67" class="C3_input"></td>					<td class="C3_td"><input value="18.8" class="C6_input"></td>					<td class="C3_td"><input value="2983" class="C6_input"></td>				</tr>				<tr class="mdoelC5Correct0s0i5">					<td class="C3_td" nowrap="nowrap">(B)</td>					<td class="C3_td"><input value="1.87" class="C3_input"></td>					<td class="C3_td"><input value="2.6" class="C6_input"></td>					<td class="C3_td"><input value="857" class="C6_input"></td>				</tr>				<tr class="mdoelC5Correct0s0i5" style="display: none;">					<td class="C3_td" nowrap="nowrap">(C)</td>					<td class="C3_td"><input value="" class="C3_input"></td>					<td class="C3_td"><input value="" class="C3_input"></td>					<td class="C3_td"><input value="" class="C3_input"></td>				</tr>				<tr class="mdoelC5Wrong0s0i5" style="display: none;">					<td class="C3_td" colspan="4" rowspan="3">						Possible reason:<br>						1. The validity or contamination of gel extraction kit and DD water.<br>						2. The validity of enzyme and buffer<br>						3. Incomplete dissolution of agar gel<br>					</td>					<td class="C3_td" rowspan="4">						<input type="button" value="Wrong" class="C6_button" name="0s0i5">					</td>				</tr>				<tr class="mdoelC5Wrong0s0i5" style="display: none;">				</tr>				<tr class="mdoelC5Wrong0s0i5" style="display: none;">				</tr>				<tr class="mdoelC5Wrong0s0i5" style="display: none;">				</tr>			</tbody></table>			<input type="hidden" id="i0s0i57" value="1"></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s0i6">2013/7/30 09:23:12</td>			<td class="M1_exp_item_content">			<table id="i0s0i6" class="C7">				<tbody><tr>					<th class="C3_td_title" colspan="3">						Ligation					</th>					<td rowspan="8" class="C3_td_textarea">						<textarea class="C3_textarea" id="i0s0i60">						</textarea>					</td>				</tr>				<tr class="C7_tr_AB">					<th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th>					<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>				</tr>				<tr class="C7_tr_AB">					<td class="C3_td"></td>					<th class="C3_td">Recommend</th>					<th class="C3_td">Actual use</th>				</tr>				<tr class="C7_tr_AB">					<th class="C3_td" id="i0s0i626">A (vector) /μL</th>					<td class="C3_td"><input value="1.1061817844701252" class="C3_input"></td>					<td class="C3_td"><input value="1" class="C3_input"></td>				</tr>				<tr class="C7_tr_AB">					<th class="C3_td" id="i0s0i627">B (insert) /μL</th>					<td class="C3_td"><input value="6.893818215529875" class="C3_input"></td>					<td class="C3_td"><input value="6" class="C3_input"></td>				</tr>				<tr class="C7_tr_AB">					<th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>					<td class="C3_td"><input value="1" class="C7_blur_2"></td>					<td class="C3_td"><input value="1" class="C3_input"></td>				</tr>				<tr class="C7_tr_AB">					<th class="C3_td">ligase /μL</th>					<td class="C3_td"><input value="1" class="C7_blur_2"></td>					<td class="C3_td"><input value="1" class="C3_input"></td>				</tr>																				<tr class="C7_tr_ABC" style="display: none;">					<th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th>					<td class="C3_td">Recommend</td>					<td class="C3_td">Actual use</td>				</tr>				<tr class="C7_tr_ABC" style="display: none;">					<td class="C3_td">A /μL</td>					<td class="C3_td"><input value="1" class="C7_input_C"></td>					<td class="C3_td"><input value="" class="C3_input"></td>				</tr>				<tr class="C7_tr_ABC" style="display: none;">					<td class="C3_td">B /μL</td>					<td class="C3_td"><input value="1" class="C7_input_C"></td>					<td class="C3_td"><input value="" class="C3_input"></td>				</tr>				<tr class="C7_tr_ABC" style="display: none;">					<td class="C3_td">C /μL</td>					<td class="C3_td"><input value="1" class="C7_input_C"></td>					<td class="C3_td"><input value="" class="C3_input"></td>				</tr>				<tr class="C7_tr_ABC" style="display: none;">					<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>					<td class="C3_td"><input value="1" class="C7_input_C"></td>					<td class="C3_td"><input value="" class="C3_input"></td>				</tr>				<tr class="C7_tr_ABC" style="display: none;">					<td class="C3_td">ligase /μL</td>					<td class="C3_td"><input value="1" class="C7_input_C"></td>					<td class="C3_td"><input value="" class="C3_input"></td>				</tr>				<tr class="C7_tr_ABC" style="display: none;">					<td class="C3_td">H<sub>2</sub>O /μL</td>					<td class="C3_td"><input value="20" class="C7_input_C"></td>					<td class="C3_td"><input value="" class="C3_input"></td>				</tr>			</tbody></table></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s0i7">2013/7/31 09:23:55</td>			<td class="M1_exp_item_content"><textarea id="i0s0i7" class="C0" rows="1">Transformation</textarea></td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="AI-2M19" id="sb0s1">			<button class="M1_exp_step_name_button"><input value="AI-2M19" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/8/1 09:24:26--Ruosang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s1i0">2013/8/1 09:24:42</td>			<td class="M1_exp_item_content">			<table id="i0s1i0" class="C8">				<tbody><tr>					<th colspan="2" class="C8_td_title">						Start to Ligate Plasmids					</th>				</tr>				<tr>					<td>						<table class="C8">							<tbody><tr>								<th colspan="3" class="C8_td_title">									choose plasmid (A)				</th>							</tr>							<tr>								<th rowspan="2" class="C3_td">									plasmid								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="AI2M19 1 (6)" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									location								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									type								</th>								<td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									part-only								</th>								<th class="C3_td">									sequence								</th>								<td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="1767" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									backbone								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="psb1a2" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="2079" class="C3_input"></td>							</tr>						</tbody></table>					</td>					<td>						<table class="C8">							<tbody><tr>								<th colspan="3" class="C8_td_title">									choose plasmid (B)				</th>							</tr>							<tr>								<th rowspan="2" class="C3_td">									plasmid								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="AI2M19 2 (6)" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									location								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									type								</th>								<td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									part-only								</th>								<th class="C3_td">									sequence								</th>								<td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="1767" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									backbone								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="psb1a2" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="2079" class="C3_input"></td>							</tr>						</tbody></table>					</td>					<td style="display: none;">						<table class="C8">							<tbody><tr>								<th colspan="3" class="C8_td_title">									choose plasmid (C)				</th>							</tr>							<tr>								<th rowspan="2" class="C3_td">									plasmid								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									location								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									type								</th>								<td class="C3_td" colspan="2"><input value="" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									part-only								</th>								<th class="C3_td">									sequence								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									backbone								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>						</tbody></table>					</td>				</tr>				<tr>					<td class="C3_td" colspan="2">						<input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s1i0">						<input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s1i0" style="display: none;">					</td>				</tr>			</tbody></table></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s1i1">2013/8/1 09:25:15</td>			<td class="M1_exp_item_content">				<table id="i0s1i1" class="C4">					<tbody><tr>						<th colspan="3">Plasmid Extraction</th>						<td rowspan="9" class="C4_td_textarea">							<textarea class="C3_textarea" id="i0s1i19">						</textarea></td>					</tr>					<tr>						<td class="C3_td">      </td>						<th class="C3_td">A<sub>260/280</sub></th>						<th class="C3_td">ng/μL</th>					</tr>					<tr>						<td class="C3_td" align="center">(A)</td>						<td class="C3_td"><input value="1.73" class="C3_input"></td>						<td class="C3_td"><input value="30.2" class="C3_input"></td>					</tr>					<tr>						<td class="C3_td" align="center">(B)</td>						<td class="C3_td"><input value="1.87" class="C3_input"></td>						<td class="C3_td"><input value="41.3" class="C3_input"></td>					</tr>					<tr class="C4_tr_C" style="display: none;">						<td class="C3_td" align="center">(C)</td>						<td class="C3_td"><input value="" class="C3_input"></td>						<td class="C3_td"><input value="" class="C3_input"></td>					</tr>					<tr>						<th class="C3_td" colspan="3">							<button class="C8_button" id="i0s1i112" name="#i0s1i1">								Evaluate							</button>						</th>					</tr>					<tr style="display: none;">						<td colspan="3">							<table class="C3">								<tbody><tr>									<td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td>									<td class="C3_td">great</td>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>								<tr>									<td class="C3_td"><input value="" class="C3_input"></td>									<td class="C3_td">fine</td>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>								<tr>									<td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td>									<td class="C3_td" rowspan="2">not good</td>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>								<tr>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>							</tbody></table>						</td>					</tr>				</tbody></table></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib0s1i2">2013/8/1 09:25:39</td>			<td class="M1_exp_item_content">	<table id="i0s1i2" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i0s1i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s1i213.8.1 18AI-2M19 (2,3).jpg"><br>				<form id="i0s1i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i0s1i22" form="i0s1i24" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t1" form="i0s1i24">				<input name="id" type="hidden" value="i0s1i2" form="i0s1i24">				<input name="oldgraph" type="hidden" id="i0s1i25" form="i0s1i24" value="t1i0s1i213.8.1 18AI-2M19 (2,3).jpg">				<input id="i0s1i26" type="submit" value="submit" form="i0s1i24" class="C1_button2" disabled="">				<div id="i0s1i23" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i0s1i21" class="C1_text">AI2M19 1 M500 2
@@ Line 40: / Line 41: @@
 			pBAD-2M19
 		</div>
-		<div class="dataOutput">
+		<div class="dataOutput2">
 				<div class="M1_exp_stepInfo" title="pBAD" id="sb1s0">			<button class="M1_exp_step_name_button"><input value="pBAD" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/8/3 18:01:27--Ruosang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib1s0i0">2013/8/3 18:01:51</td>			<td class="M1_exp_item_content">			<table id="i1s0i0" class="C8">				<tbody><tr>					<th colspan="2" class="C8_td_title">						Start to Ligate Plasmids					</th>				</tr>				<tr>					<td>						<table class="C8">							<tbody><tr>								<th colspan="3" class="C8_td_title">									choose plasmid (A)				</th>							</tr>							<tr>								<th rowspan="2" class="C3_td">									plasmid								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="pbad" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									location								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									type								</th>								<td class="C3_td" colspan="2"><input value="pbad" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									part-only								</th>								<th class="C3_td">									sequence								</th>								<td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="130" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									backbone								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="psb1a3" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="2155" class="C3_input"></td>							</tr>						</tbody></table>					</td>					<td>						<table class="C8">							<tbody><tr>								<th colspan="3" class="C8_td_title">									choose plasmid (B)				</th>							</tr>							<tr>								<th rowspan="2" class="C3_td">									plasmid								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									location								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									type								</th>								<td class="C3_td" colspan="2"><input value="" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									part-only								</th>								<th class="C3_td">									sequence								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									backbone								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>						</tbody></table>					</td>					<td style="display: none;">						<table class="C8">							<tbody><tr>								<th colspan="3" class="C8_td_title">									choose plasmid (C)				</th>							</tr>							<tr>								<th rowspan="2" class="C3_td">									plasmid								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									location								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									type								</th>								<td class="C3_td" colspan="2"><input value="" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									part-only								</th>								<th class="C3_td">									sequence								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th rowspan="2" class="C3_td">									backbone								</th>								<th class="C3_td">									name								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>							<tr>								<th class="C3_td">									length								</th>								<td class="C3_td"><input value="" class="C3_input"></td>							</tr>						</tbody></table>					</td>				</tr>				<tr>					<td class="C3_td" colspan="2">						<input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i1s0i0">						<input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i1s0i0" style="display: none;">					</td>				</tr>			</tbody></table></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib1s0i1">2013/8/3 18:02:03</td>			<td class="M1_exp_item_content">				<table id="i1s0i1" class="C4">					<tbody><tr>						<th colspan="3">Plasmid Extraction</th>						<td rowspan="9" class="C4_td_textarea">							<textarea class="C3_textarea" id="i1s0i19">						</textarea></td>					</tr>					<tr>						<td class="C3_td">      </td>						<th class="C3_td">A<sub>260/280</sub></th>						<th class="C3_td">ng/μL</th>					</tr>					<tr>						<td class="C3_td" align="center">(A)</td>						<td class="C3_td"><input value="1.88" class="C3_input"></td>						<td class="C3_td"><input value="19" class="C3_input"></td>					</tr>					<tr>						<td class="C3_td" align="center">(B)</td>						<td class="C3_td"><input value="" class="C3_input"></td>						<td class="C3_td"><input value="" class="C3_input"></td>					</tr>					<tr class="C4_tr_C" style="display: none;">						<td class="C3_td" align="center">(C)</td>						<td class="C3_td"><input value="" class="C3_input"></td>						<td class="C3_td"><input value="" class="C3_input"></td>					</tr>					<tr>						<th class="C3_td" colspan="3">							<button class="C8_button" id="i1s0i112" name="#i1s0i1">								Evaluate							</button>						</th>					</tr>					<tr style="display: none;">						<td colspan="3">							<table class="C3">								<tbody><tr>									<td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td>									<td class="C3_td">great</td>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>								<tr>									<td class="C3_td"><input value="" class="C3_input"></td>									<td class="C3_td">fine</td>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>								<tr>									<td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td>									<td class="C3_td" rowspan="2">not good</td>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>								<tr>									<td class="C3_td"><input value="" class="C3_input"></td>								</tr>							</tbody></table>						</td>					</tr>				</tbody></table></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib1s0i2">2013/8/3 18:03:36</td>			<td class="M1_exp_item_content">		<table class="C5" id="i1s0i2">			<tbody><tr>				<th colspan="3" id="i1s0i229" class="C5_th_C">Extract plasmid</th>				<td rowspan="10" class="C3_td_textarea">					<textarea class="C3_textarea" id="i1s0i20">					</textarea>				</td>			</tr>			<tr>				<th class="C5_canvas_td" colspan="3" id="i1s0i230">					<div class="C5_canvas_matrix">					</div>					<div class="C5_TEXT1">EX</div>					<div class="C5_TEXT2">SP</div>					<input type="button" class="C5_canvas_button1" id="i1s0i21" name="#i1s0i2" value="pbad (A)">					<input type="button" class="C5_canvas_button2" id="i1s0i22" name="#i1s0i2" value=" (B)">					<input type="button" class="C5_canvas_button3" id="i1s0i23" name="#i1s0i2" value="pbad (A)">				</th>			</tr>				<tr>					<th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th>					<th class="C3_td">(A)</th>					<th class="C3_td">(B)</th>					<th class="C3_td_C5_C" style="display: none;">(C)</th>				</tr>				<tr>					<th class="C3_td">Plasmid</th>					<td class="C3_td"><input value="40" class="C3_input"></td>					<td class="C3_td"><input value="0" class="C3_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td>				</tr>				<tr>					<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>					<td class="C3_td"><input value="10" class="C3_input"></td>					<td class="C3_td"><input value="10" class="C3_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td>				</tr>				<tr>					<th class="C3_td"><em>Eco</em>R I /μL</th>					<td class="C3_td"><input value="0" class="C5_input"></td>					<td class="C3_td"><input value="0" class="C5_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td>				</tr>				<tr>					<th class="C3_td"><em>Xba</em> I /μL</th>					<td class="C3_td"><input value="0" class="C5_input"></td>					<td class="C3_td"><input value="5" class="C5_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td>				</tr>				<tr>					<th class="C3_td"><em>Spe</em> I /μL</th>					<td class="C3_td"><input value="5" class="C5_input"></td>					<td class="C3_td"><input value="0" class="C5_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td>				</tr>				<tr>					<th class="C3_td"><em>Pst</em> I /μL</th>					<td class="C3_td"><input value="5" class="C5_input"></td>					<td class="C3_td"><input value="5" class="C5_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td>				</tr>				<tr>					<th class="C3_td">H<sub>2</sub>0 /μL</th>					<td class="C3_td"><input value="40" class="C3_input"></td>					<td class="C3_td"><input value="80" class="C3_input"></td>					<td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td>					<input type="hidden" id="i1s0i226" value="0">					<input type="hidden" id="i1s0i227" value="1">					<input type="hidden" id="i1s0i228" value="0">				</tr>		</tbody></table></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib1s0i3">2013/8/3 18:03:49</td>			<td class="M1_exp_item_content">	<table id="i1s0i3" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i1s0i30" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s0i313.8.3 牛津样，pBAD.jpg"><br>				<form id="i1s0i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i1s0i32" form="i1s0i34" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t1" form="i1s0i34">				<input name="id" type="hidden" value="i1s0i3" form="i1s0i34">				<input name="oldgraph" type="hidden" id="i1s0i35" form="i1s0i34" value="t1i1s0i313.8.3 牛津样，pBAD.jpg">				<input id="i1s0i36" type="submit" value="submit" form="i1s0i34" class="C1_button2" disabled="">				<div id="i1s0i33" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i1s0i31" class="C1_text">OTHER SAMPLE M(500) PBAD
@@ Line 81: / Line 82: @@
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