Team:Penn/MethylaseOverview
From 2013.igem.org
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Now that the MaGellin assay was validated for non-specific methylases, we were ready to test site-specific methylases. These are fusion proteins, a DNA binding domain linked to a methylase by a serine glycine chain. We used the prokaryotic methylase M.SssI for all of our studies (BBa_K1128000). | Now that the MaGellin assay was validated for non-specific methylases, we were ready to test site-specific methylases. These are fusion proteins, a DNA binding domain linked to a methylase by a serine glycine chain. We used the prokaryotic methylase M.SssI for all of our studies (BBa_K1128000). | ||
- | We wanted to first recapitulate published results with a zinc finger binding domain, and then characterize our two novel site-specific methylases: using the TALE and CRISPR/Cas binding domains. | + | <p>We wanted to first recapitulate published results with a zinc finger binding domain, and then characterize our two novel site-specific methylases: using the TALE and CRISPR/Cas binding domains. |
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INSERT TABLE COMPARISON HERE | INSERT TABLE COMPARISON HERE |
Revision as of 11:38, 27 October 2013
Site Specific Methylase
We wanted to first recapitulate published results with a zinc finger binding domain, and then characterize our two novel site-specific methylases: using the TALE and CRISPR/Cas binding domains. INSERT TABLE COMPARISON HERE