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| | {{Template:Team:Bonn}} | | {{Template:Team:Bonn}} |
| - | {{Template:Team:Bonn:menu}} | + | {{Template:Team:Bonn:NetworkData}} |
| | + | {{Template:Team:Bonn:Network}} |
| | + | {{Template:Team:Bonn:NetworkTop}} |
| | + | {{Template:Team:Bonn:NetworkBottom}} |
| | + | {{Template:Team:Bonn:Menu}} |
| | + | {{Template:Team:Bonn:aboutus}} |
| | <html> | | <html> |
| | + | <body onload=showElements(0,0,"mainpage")> |
| | + | <div id="content-page"> |
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| - | <div class="page-top">
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| - | <div id="page-top-slideshow">
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| - | <div id="page-top-intro" class="page-top-slide">
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| - | <div style="width:950px;">
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| - | Regulating protein activity is important throughout lifescience research. Even though there are several
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| - | systems available for regulation of proteins all suffer from certain disadvantages. </br>
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| - | <span style="font-style:italic">Aiming to overcome all of these drawbacks we engineer a novel system for
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| - | light dependend regulation of protein degradation resulting in a rapid change of protein activity.
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| - | Our system can be not only of great importance for fundamental science to investigate protein function but
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| - | also for accurate control of bioreactors and biological machines.</span>
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| - | </div>
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| - | <div style="margin:auto;">
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| - | <img src="https://static.igem.org/mediawiki/2013/f/f7/IGEM_project_LightProtAct.png" height="170px" >
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| - | <div id="page-top-game" class="page-top-slide">
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| - | <a href="https://2013.igem.org/Team:Bonn/HP/game/"><img src="https://static.igem.org/mediawiki/2013/f/f8/Bonn_game_titelpic.png"></a>
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| - | <div id="page">
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| - | <table id="page-table">
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| - | <td rowspan="10" colspan="4" >
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| - | <div class="page-box" id="page-box1">
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| - | <img src="https://static.igem.org/mediawiki/2013/6/67/Bonn_Obiwanecoli001.png">
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| - | <div class="page-box-bottom" id="page-box1-bottom">
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| - | <div class="page-box-bottom-headline" id="page-box1-bottom-headline">
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| - | Project
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| - | Making versatile control of biological machines easily possible for everybody we engineer a system for
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| - | light dependend control of protein activity per protein degradation.</br>
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| - | Harnessing the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused
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| - | protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is
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| - | activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br>
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| - | Modelling of the light dependency of protein degradation will even make simple and accurate control of
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| - | protein activity for any desired level of activity possible.</br>
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| - | As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.</br>
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| - | </div>
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| - | <div class="page-box-bottom-links">
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| - | Find out more about </br>
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| - | <div style="margin: 5px 0 0 10px">
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| - | split SspB </br>
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| - | light inducible heterodimerisation </br>
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| - | our protocols </br>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | </td>
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| - | <td rowspan="5" colspan="6" >
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| - | <div class="page-box" id="page-box2">
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| - | <img src="https://static.igem.org/mediawiki/2013/b/b1/Bonn_Masteryoda001.png" height="230px">
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| - | <div class="page-box-bottom" id="page-box2-bottom">
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| - | <div class="page-box-bottom-headline" id="page-box2-bottom-headline">
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| - | Background
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| - | </div>
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| - | <div class="page-box-bottom-text">
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| - | To enable protein degradation we decided on one proteases system (ClpXP), which allows specific degradation
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| - | of proteins, is highly conserved and well established.
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| - | For light dependency we compared several systems to finally find the best suited one. We created a
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| - | comprehensive overview of all systems making artificially light control possible so that everyone can
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| - | easily choose the optimal system for his approach.
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| - | </div>
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| - | <div class="page-box-bottom-links">
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| - | Find out more about </br>
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| - | <div style="margin: 5px 0 0 10px">
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| - | the ClpXP protease System </br>
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| - | our Comparison of several light sensitive systems</br>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | </td>
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| - | <div class="page-box" id="page-box3">
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| - | <img src="https://static.igem.org/mediawiki/2013/3/33/Bonn_Darthcherry001.png" height="230px">
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| - | <div class="page-box-bottom" id="page-box3-bottom">
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| - | <div class="page-box-bottom-headline" id="page-box3-bottom-headline">
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| - | Human Practice
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| - | </div>
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| - | <div class="page-box" id="page-box4">
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| - | <img src="https://static.igem.org/mediawiki/2013/4/4e/Bonn_PrincLayer.png" width="800px">
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| - | <div class="page-box-bottom" id="page-box4-bottom">
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| - | <div class="page-box-bottom-headline" id="page-box4-bottom-headline">
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| - | Team
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| - | </div>
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| - | </div>
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| - | </div>
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| | </div> | | </div> |
| - | </div> <!-- close page -->
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| - | <!-- Ultra-crap-hack: Close two div containers which have been opened in an included template (apparently) -->
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| - | <!--
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| - | TODO: Investigate what we are closing here, and if we can do better (if not, DOCUMENT THIS somewhere and make
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| - | it known to everyone in our Web/PR/Etc. teams.
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| - | --- Matthias
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| - | -->
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| | </div> | | </div> |
| | </div> | | </div> |
| - | <script>
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| | </html> | | </html> |
| | {{Template:Team:Bonn:sponsors}} | | {{Template:Team:Bonn:sponsors}} |
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