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| | {{Template:Team:Bonn}} | | {{Template:Team:Bonn}} |
| - | {{Template:Team:Bonn:menu}} | + | {{Template:Team:Bonn:NetworkData}} |
| | + | {{Template:Team:Bonn:Network}} |
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| | + | {{Template:Team:Bonn:NetworkBottom}} |
| | + | {{Template:Team:Bonn:Menu}} |
| | + | {{Template:Team:Bonn:aboutus}} |
| | <html> | | <html> |
| | + | <body onload=showElements(0,0,"mainpage")> |
| | + | <div id="content-page"> |
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| - | <div class="page-top">
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| - | <div id="page-top-slideshow">
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| - | <div id="page-top-intro" class="page-top-slide">
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| - | <div style="margin-left:200px;margin-top:-30px">
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| - | <img src="https://static.igem.org/mediawiki/2013/f/f7/IGEM_project_LightProtAct.png" height="170px" >
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| - | </div>
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| - | <div style="width:800px;margin:auto;">
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| - | Regulating protein activity is important throughout lifescience research. Even though there are several
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| - | systems available for regulation of proteins all suffer from certain disadvantages. </br>
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| - | <span style="font-style:italic">Aiming to overcome all of these drawbacks we engineer a novel system for
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| - | light dependend regulation of protein degradation resulting in a rapid change of protein activity.
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| - | Our system can be not only of great importance for fundamental science to investigate protein function but
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| - | also for accurate control of bioreactors and biological machines.</span>
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| - | <div id="page-top-game" class="page-top-slide">
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| - | <a href="https://2013.igem.org/Team:Bonn/HP/game/"><img src="https://static.igem.org/mediawiki/2013/f/f8/Bonn_game_titelpic.png" width="950px"></a>
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| - | <div id="page">
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| - | <table id="page-table">
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| - | <td rowspan="10" colspan="4" >
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| - | <div class="page-box" id="page-box1">
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| - | <img src="https://static.igem.org/mediawiki/2013/6/67/Bonn_Obiwanecoli001.png">
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| - | <div class="page-box-bottom" id="page-box1-bottom">
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| - | <div class="page-box-bottom-headline" id="page-box1-bottom-headline">
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| - | Project
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| - | Making versatile control of biological machines easily possible for everybody we engineer a system for
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| - | light dependend control of protein activity per protein degradation.</br>
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| - | Harnessing the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused
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| - | protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is
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| - | activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br>
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| - | Modelling of the light dependency of protein degradation will even make simple and accurate control of
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| - | protein activity for any desired level of activity possible.</br>
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| - | As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.</br>
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| - | </div>
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| - | <div class="page-box-bottom-links">
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| - | Find out more about </br>
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| - | split SspB </br>
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| - | light inducible heterodimerisation </br>
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| - | our protocols </br>
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| - | </div>
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| - | <div class="page-box" id="page-box2">
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| - | <img src="https://static.igem.org/mediawiki/2013/b/b1/Bonn_Masteryoda001.png" height="230px">
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| - | <div class="page-box-bottom-headline" id="page-box2-bottom-headline">
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| - | Background
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| - | </div>
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| - | <div class="page-box-bottom-text">
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| - | To enable protein degradation we decided on one proteases system (ClpXP), which allows specific degradation
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| - | of proteins, is highly conserved and well established.
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| - | For light dependency we compared several systems to finally find the best suited one. We created a
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| - | comprehensive overview of all systems making artificially light control possible so that everyone can
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| - | easily choose the optimal system for his approach.
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| - | </div>
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| - | <div class="page-box-bottom-links">
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| - | Find out more about </br>
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| - | <div style="margin: 5px 0 0 10px">
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| - | the ClpXP protease System </br>
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| - | our Comparison of several light sensitive systems</br>
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| - | </div>
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| - | </div>
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| - | <div class="page-box" id="page-box3">
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| - | <img src="https://static.igem.org/mediawiki/2013/3/33/Bonn_Darthcherry001.png" width="445px">
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| - | <div class="page-box-bottom" id="page-box3-bottom">
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| - | <div class="page-box-bottom-headline" id="page-box3-bottom-headline">
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| - | Human Practice
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| - | The major goal of our Human Practice work is to make Synthethic Biology easily understandable and intresting for everybody.</br>
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| - | For that purpose we introduce Synthetic Biology and our project to the generald public via developing a comic series, programming a computer game and conduct several presentations in schools all over germany.
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| - | Getting the general public even more in touch with science we furthermore planned a Science Slam.
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| - | </div>
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| - | <div class="page-box-bottom-links">
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| - | Find out more about </br>
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| - | <div style="margin: 5px 0 0 10px">
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| - | the ClpXP protease System </br>
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| - | our Comparison of several light sensitive systems</br>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | </td>
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| - | <td rowspan="4" colspan="10" >
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| - | <div class="page-box" id="page-box4">
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| - | <img src="https://static.igem.org/mediawiki/2013/4/4e/Bonn_PrincLayer.png" width="800px">
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| - | <div class="page-box-bottom" id="page-box4-bottom">
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| - | <div class="page-box-bottom-headline" id="page-box4-bottom-headline">
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| - | Team
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| - | <div class="page-box-bottom-text">
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| - | We are the iGEM team from Bonn consisting of 22 students from the fields Biomedicine, Biology, Biotechnology and Mathematics. Organization and planning of our project is carried out by veternary members, already have been part of the last year iGEM Team. A large portion of our team consists of first year bachelor students making our team highly dynamic, innovative and motivated.
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| - | Our lab is part of the Life and Medical Sciences Institute in Bonn.
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| - | We would like to thank all of our sponsors for making this project possible.
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| - | </div>
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| - | <div class="page-box-bottom-links">
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| - | Find out more about </br>
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| - | <div style="margin: 5px 0 0 10px">
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| - | the ClpXP protease System </br>
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| - | our Comparison of several light sensitive systems</br>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | </td>
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| - | </table>
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| | </div> | | </div> |
| - | </div> <!-- close page -->
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| - | <!-- Ultra-crap-hack: Close two div containers which have been opened in an included template (apparently) -->
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| - | <!--
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| - | TODO: Investigate what we are closing here, and if we can do better (if not, DOCUMENT THIS somewhere and make
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| - | it known to everyone in our Web/PR/Etc. teams.
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| - | --- Matthias
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| - | -->
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| | </html> | | </html> |
| | {{Template:Team:Bonn:sponsors}} | | {{Template:Team:Bonn:sponsors}} |
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