Team:SydneyUni Australia

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== '''Brief Project Description''' ==
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*1,2-dichloroethane (DCA) is part of a family of chlorinated hydrocarbons that are primarily derived from industrial solvents such as tetrachloroethene (PCE) and trichloroethene (TCE). Some of these organochlorines are toxic, carcinogenic and generally nasty.
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*DCA is a soluble and mobile contaminant of the groundwater in Botany Bay, Sydney, but also elsewhere around the world.
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*Conventional treatment involves significant costs, such as the pumping and heat-stripping of groundwater at Botany Bay. A biological alternative may be cheaper and more effective.
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*A suite of techniques (including sampling of contaminated sites, growth in bioreactors with selective conditions and protein engineering) have led to an understanding that there are two primary pathways of DCA-degradation (Fig. 1).
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'''Goals'''
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*Construct a BioBrick-compatible vector inspired by the broad host range vector, pBBR1MCS2.
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*Construct and compare two of the proposed pathways of DCA biodegradation.
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*Characterise the components of the DCA-degradation pathway for admission into the Registry of Standard Parts.
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*(If we get time!) Demonstrate the integration of our the pathway in the chromosome of Pseudomonas stutzeri via natural transformation and site-specific recombination.
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We’re a team of eight excited undergraduates from Sydney University, Australia, with huge interests in biochemistry and bioengineering. We’re new to iGEM this year, but fingers crossed, we'll do well and have a blast while doing it!
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<a href="https://2013.igem.org/Team:SydneyUni_Australia/Team">
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[[File:DCApathwaysHartman.jpg|center]]
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Most of our work involved trying to build a pathway to degrade <a href="https://2013.igem.org/Team:SydneyUni_Australia/Project/Background#Chlorinated_Hydrocarbons"> dichloroethane</a> (DCA). This contaminant has leaked into the groundwater in <a href="https://2013.igem.org/Team:SydneyUni_Australia/Project/Background">Botany</a>, Sydney, and elsewhere around the world. Our aim is to assemble a pathway for DCA degradation.
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<a href="https://2013.igem.org/Team:SydneyUni_Australia/Project/Background">
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We created a science-writing competition for Australian high-school students. By providing an online platform for students and teachers to explore Synthetic Biology we hope to inspire imagination and critical reflection about our future.
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<a href="https://2013.igem.org/Team:SydneyUni_Australia/Strange_Nature">
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{{Team:SydneyUni_Australia/Footer}}

Latest revision as of 13:49, 27 October 2013

SydneyUniversity Top Banner.jpg SydneyUniversity Bottom Banner.jpg


We’re a team of eight excited undergraduates from Sydney University, Australia, with huge interests in biochemistry and bioengineering. We’re new to iGEM this year, but fingers crossed, we'll do well and have a blast while doing it!
Most of our work involved trying to build a pathway to degrade dichloroethane (DCA). This contaminant has leaked into the groundwater in Botany, Sydney, and elsewhere around the world. Our aim is to assemble a pathway for DCA degradation.
We created a science-writing competition for Australian high-school students. By providing an online platform for students and teachers to explore Synthetic Biology we hope to inspire imagination and critical reflection about our future.

With thanks to:



Retrieved from "http://2013.igem.org/Team:SydneyUni_Australia"