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Talk:Team:Groningen/Labwork/17 July 2013
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''' Mirjam''' | ''' Mirjam''' | ||
| - | Check of the restriction done yesterday. This revealed that 5/7 colonies did contain the transformant. The cells of the fourth plate started to grow, so a mini prep analysis is performed. A restriction analysis is done with EcoRV. | + | <br/>Check of the restriction done yesterday. This revealed that 5/7 colonies did contain the transformant. The cells of the fourth plate started to grow, so a mini prep analysis is performed. A restriction analysis is done with EcoRV. |
A second confirmation of the constructs is done using PCR. | A second confirmation of the constructs is done using PCR. | ||
Revision as of 09:22, 17 July 2013
Mirjam
Check of the restriction done yesterday. This revealed that 5/7 colonies did contain the transformant. The cells of the fourth plate started to grow, so a mini prep analysis is performed. A restriction analysis is done with EcoRV.
A second confirmation of the constructs is done using PCR.
