Team:DTU-Denmark/Notebook/14 June 2013
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*transformation of [http://parts.igem.org/Part:BBa_I0500 Biobrick I0500] on Amp instead of Kan | *transformation of [http://parts.igem.org/Part:BBa_I0500 Biobrick I0500] on Amp instead of Kan | ||
*transformation of [http://parts.igem.org/Part:BBa_K564012 Biobrick K564012] on CAM | *transformation of [http://parts.igem.org/Part:BBa_K564012 Biobrick K564012] on CAM | ||
+ | * we used two different batches of competent cells and 200 ul of cells instead of 50ul | ||
+ | * we incubated on LB rather than SOC | ||
Revision as of 12:31, 17 July 2013
14 June 2013
208
- PCR was run
- transformation of [http://parts.igem.org/Part:BBa_I0500 Biobrick I0500] on Amp instead of Kan
- transformation of [http://parts.igem.org/Part:BBa_K564012 Biobrick K564012] on CAM
- we used two different batches of competent cells and 200 ul of cells instead of 50ul
- we incubated on LB rather than SOC
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