Team:Groningen/Labwork/17 July 2013

From 2013.igem.org

(Difference between revisions)
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<br>The ''E. coli'' cells with the BBa_k818000 that were to grow overnight didn't grow.
<br>The ''E. coli'' cells with the BBa_k818000 that were to grow overnight didn't grow.
<br>The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.
<br>The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.
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<br> Made -80 C stock of the Munich backbone BBa_K823023, in tray iGEM 2012 A, position 26.
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<br> Inoculated the BBa_818000, made 5 tubes
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<table border = "1">
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<tr>
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<td>Sample</td>
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<td>Composition</td>
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</tr>
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<tr>
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<td>1</td>
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<td>4mL LB + 8uL Amp</td>
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</tr>
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<tr>
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<td>2</td>
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<td>4mL LB + 8uL Amp</td>
 +
</tr>
 +
<tr>
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<td>3</td>
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<td>4mL LB + 8uL Amp (negative control)</td>
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</tr>
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<tr>
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<td>4</td>
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<td>4mL LB</td>
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</tr>
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<tr>
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<td>5</td>
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<td>4mL LB (negative control)</td>
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</tr>
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</table>

Revision as of 14:40, 17 July 2013

Mirjam
Check of the restriction done yesterday. This revealed that 5/7 colonies did contain the transformant. The cells of the fourth plate started to grow, so a mini prep analysis is performed. A restriction analysis is done with EcoRV.

A second confirmation of the constructs is done using PCR.

Inne
The E. coli cells with the BBa_k818000 that were to grow overnight didn't grow.
The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.


Made -80 C stock of the Munich backbone BBa_K823023, in tray iGEM 2012 A, position 26.


Inoculated the BBa_818000, made 5 tubes

Sample Composition
1 4mL LB + 8uL Amp
2 4mL LB + 8uL Amp
3 4mL LB + 8uL Amp (negative control)
4 4mL LB
5 4mL LB (negative control)
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