Team:Shenzhen BGIC ATCG/parts

From 2013.igem.org

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(Created page with "<!---------------------- Title: Parts Team: Shenzhen_BGIC_ATCG Author: BGI_K2 Twitter: @BGI_K2 Email: im.ss.kk@gmail.com Please contact me if you would like to use this sc...")
 
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<ul id="myGallery1">
<ul id="myGallery1">
-
           <li><img src="https://static.igem.org/mediawiki/2012/b/b5/Yao.result.jpg" />
+
           <li><img src="https://static.igem.org/mediawiki/2013/b/b9/Blank-01.png" /><li>
-
          <li><img src="https://static.igem.org/mediawiki/2012/7/77/Yao.012-001.jpg" />
+
-
            <li><img src="https://static.igem.org/mediawiki/2012/1/1b/Result_yao_genome_p2.jpg" />
+
</ul></div>
</ul></div>
     <div id="box1" class="box">
     <div id="box1" class="box">
-
        <h3>Best BioBricks</h3>
+
      <h3>Best BioBricks</h3>
 +
<h4>Best BioBrick (Natural/Collection), BBa_K1051303, BBa_K1051300 ~ BBa_K1051306</h4>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051303"><p>K1051303</p>
 +
<img src="https://static.igem.org/mediawiki/2013/0/0c/K1051303.png"></a>
 +
<p>K1051300</p>
 +
<img src="https://static.igem.org/mediawiki/2013/8/8d/K1051300.png">
 +
<p>K1051301</p>
 +
<img src="https://static.igem.org/mediawiki/2013/c/c0/K1051301.png">
 +
<p>K1051302</p>
 +
<img src="https://static.igem.org/mediawiki/2013/c/c6/K1051302.png">
 +
<p>K1051304</p>
 +
<img src="https://static.igem.org/mediawiki/2013/f/fa/K1051304.png">
 +
<p>K1051305</p>
 +
<img src="https://static.igem.org/mediawiki/2013/4/49/K1051305.png">
 +
<p>K1051306</p>
 +
<img src="https://static.igem.org/mediawiki/2013/5/5d/K1051306.png">
 +
<h4>Best BioBrick (Engineered), BBa_K1051900</h4>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051900"><p>K1051900</p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/c/ce/K1051900a.png">
 +
<img src="https://static.igem.org/mediawiki/igem.org/f/fa/K1051900b.png"></a>
 +
<h4>Best Measurement, BBa_K1051257 ~ BBa_K1051259</h4>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051257"><p>K1051257</p>
 +
<img src="https://static.igem.org/mediawiki/2013/4/43/K1051257.png"></a>
 +
<p>K1051258</p>
 +
<img src="https://static.igem.org/mediawiki/2013/d/d4/K1051258.png">
 +
<p>K1051259</p>
 +
<img src="https://static.igem.org/mediawiki/2013/e/ec/K1051259.png">
 +
 
 +
 
</div>
</div>
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<div class="media">
<div class="media">
<ul id="myGallery2">
<ul id="myGallery2">
-
           <li><img src="https://static.igem.org/mediawiki/2012/b/b5/Yao.result.jpg" title="YAO.Genome"/>
+
           <li><img src="https://static.igem.org/mediawiki/2013/b/b9/Blank-01.png" /><li>
</ul></div>
</ul></div>
     <div id="box2" class="box">
     <div id="box2" class="box">
         <h3>Devices List</h3>
         <h3>Devices List</h3>
 +
<h4>Final Constructions</h4>
 +
<h5>Final Constructions of <i>E.coli</i></h5>
 +
<p>K1051910</p>
 +
<img src="https://static.igem.org/mediawiki/2013/d/d7/K1051910.png">
 +
<p>K1051911</p>
 +
<img src="https://static.igem.org/mediawiki/2013/0/0a/K1051911.png">
 +
<p>K1051912</p>
 +
<img src="https://static.igem.org/mediawiki/2013/b/bc/K1051912.png">
 +
<h5>Final Constructions of Budding Yeast</h5>
 +
<p>K1051900a</p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/c/ce/K1051900a.png">
 +
<p>K1051900b</p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/f/fa/K1051900b.png">
 +
<p>K1051901</p>
 +
<img src="https://static.igem.org/mediawiki/2013/e/e3/K1051901.png">
 +
<p>K1051902</p>
 +
<img src="https://static.igem.org/mediawiki/2013/2/2c/K1051902.png">
 +
<p>K1051903</p>
 +
<img src="https://static.igem.org/mediawiki/2013/1/11/K1051903.png">
 +
<h4>Promoter Verification</h4>
 +
<h5>Verification of <i>E.coli</i></h5>
 +
<p>K1051245</p>
 +
<img src="https://static.igem.org/mediawiki/2013/d/d8/K1051245.png">
 +
<p>K1051246</p>
 +
<img src="https://static.igem.org/mediawiki/2013/2/2b/K1051246.png">
 +
<p>K1051247</p>
 +
<img src="https://static.igem.org/mediawiki/2013/7/79/K1051247.png">
 +
<h5>Verification of Budding Yeast</h5>
 +
<p>K1051307</p>
 +
<img src="https://static.igem.org/mediawiki/2013/c/c5/K1051307.png">
 +
<p>K1051355</p>
 +
<img src="https://static.igem.org/mediawiki/2013/1/12/K1051355.png">
 +
<p>K1051356</p>
 +
<img src="https://static.igem.org/mediawiki/2013/7/74/K1051356.png">
 +
<p>K1051357</p>
 +
<img src="https://static.igem.org/mediawiki/2013/4/48/K1051357.png">
 +
<p>K1051358</p>
 +
<img src="https://static.igem.org/mediawiki/2013/d/d6/K1051358.png">
 +
<p>K1051359</p>
 +
<img src="https://static.igem.org/mediawiki/2013/6/65/K1051359.png">
 +
<p>K1051365</p>
 +
<img src="https://static.igem.org/mediawiki/2013/4/40/K1051365.png">
 +
<p>K1051366</p>
 +
<img src="https://static.igem.org/mediawiki/2013/3/33/K1051366.png">
 +
<p>K1051367</p>
 +
<img src="https://static.igem.org/mediawiki/2013/4/4f/K1051367.png">
 +
<p>K1051368</p>
 +
<img src="https://static.igem.org/mediawiki/2013/8/8a/K1051368.png">
 +
<p>K1051369</p>
 +
<img src="https://static.igem.org/mediawiki/2013/5/52/K1051369.png">
 +
<h4>Degradation Rate Measurement</h4>
 +
<h5>Measurement of <i>E.coli</i></h5>
 +
<p>K1051257</p>
 +
<img src="https://static.igem.org/mediawiki/2013/4/43/K1051257.png">
 +
<p>K1051258</p>
 +
<img src="https://static.igem.org/mediawiki/2013/d/d4/K1051258.png">
 +
<p>K1051259</p>
 +
<img src="https://static.igem.org/mediawiki/2013/e/ec/K1051259.png">
 +
<h5>Measurement of Budding Yeast</h5>
 +
<p>K1051278</p>
 +
<img src="https://static.igem.org/mediawiki/2013/b/b2/K1051278.png">
 +
<p>K1051279</p>
 +
<img src="https://static.igem.org/mediawiki/2013/1/1d/K1051279.png">
 +
<p>K1051280</p>
 +
<img src="https://static.igem.org/mediawiki/2013/1/1a/K1051280.png">
 +
<p>K1051281</p>
 +
<img src="https://static.igem.org/mediawiki/2013/5/5e/K1051281.png">
 +
<p>K1051288</p>
 +
<img src="https://static.igem.org/mediawiki/2013/8/88/K1051288.png">
 +
<h4>Targeting Peptides Verification</h4>
 +
<p>K1051150</p>
 +
<img src="https://static.igem.org/mediawiki/2013/d/db/K1051150.png">
 +
<p>K1051151</p>
 +
<img src="https://static.igem.org/mediawiki/2013/9/97/K1051151.png">
 +
<p>K1051152</p>
 +
<img src="https://static.igem.org/mediawiki/2013/0/0e/K1051152.png">
 +
<p>K1051153</p>
 +
<img src="https://static.igem.org/mediawiki/2013/c/cb/K1051153.png">
 +
<h4>CRISPRi Verification</h4>
 +
<p>K1051852</p>
 +
<img src="https://static.igem.org/mediawiki/2013/3/33/K1051852.png">
 +
<h4>Alternative Splicing Verification</h4>
 +
<p>K1051706</p>
 +
<img src="https://static.igem.org/mediawiki/2013/2/2b/K1051706.png">
 +
     </div>
     </div>
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<div class="media">
<div class="media">
<ul id="myGallery3">
<ul id="myGallery3">
-
           <li><img src="https://static.igem.org/mediawiki/2012/b/b5/Yao.result.jpg" />
+
           <li><img src="https://static.igem.org/mediawiki/2013/b/b9/Blank-01.png" /><li>
</ul></div>
</ul></div>
     <div id="box3" class="box">
     <div id="box3" class="box">
         <h3>Basic Parts</h3>
         <h3>Basic Parts</h3>
 +
<h4>Universal Parts</h4>
 +
<h5>Reporters</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051000'>BBa_K1051000</A></TD><TD>Reporter</TD><TD>Stop codon free RFP in RFC[23] standard</TD><TD >Xiang LI</TD><TD ALIGN='right'>675</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051001'>BBa_K1051001</A></TD><TD>Reporter</TD><TD>Stop codon free ECFP in RFC[23] standard</TD><TD >Xiang LI</TD><TD ALIGN='right'>716</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051002'>BBa_K1051002</A></TD><TD>Reporter</TD><TD>Stop codon free EYFP in RFC[23] standard</TD><TD >Xiang LI</TD><TD ALIGN='right'>717</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051003'>BBa_K1051003</A></TD><TD>Reporter</TD><TD>Stop codon free GFP in RFC[23] standard</TD><TD >Xiang LI</TD><TD ALIGN='right'>714</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051004'>BBa_K1051004</A></TD><TD>Reporter</TD><TD>Stop codon free mOrange in RFC[23] standard</TD><TD >Xiang LI</TD><TD ALIGN='right'>738</TD>
 +
</table>
 +
<h5>Yeast Terminators</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051006'>BBa_K1051006</A></TD><TD>Terminator</TD><TD>Saccharomycescerevisiae TBY-1 terminator with stopcodon</TD><TD >Xiang LI</TD><TD ALIGN='right'>31</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051007'>BBa_K1051007</A></TD><TD>Terminator</TD><TD>S. cerevisiae SSV7 terminator with stopcodon</TD><TD >Xiang LI</TD><TD ALIGN='right'>39</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051008'>BBa_K1051008</A></TD><TD>Terminator</TD><TD>S. cerevisiae CAA terminator with stopcodon</TD><TD >Xiang LI</TD><TD ALIGN='right'>18</TD>
 +
</table>
 +
<h5><i>E. coli</i> Terminators</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051009'>BBa_K1051009</A></TD><TD>Terminator</TD><TD>e.coli terminator ilvGEDA_T with stop codon</TD><TD >Xiang LI</TD><TD ALIGN='right'>92</TD>
 +
</table>
 +
<h4>Cyclin Promoters</h4>
 +
<h5>Yeast</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051300'>BBa_K1051300</A></TD><TD>Regulatory</TD><TD> cln2  promoter (G1 phase to S phase)</TD><TD >Kequan Lin</TD><TD ALIGN='right'>1000</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051301'>BBa_K1051301</A></TD><TD>Regulatory</TD><TD> clb2  promoter (during G2 phase)</TD><TD >Kequan Lin</TD><TD ALIGN='right'>1063</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051302'>BBa_K1051302</A></TD><TD>Regulatory</TD><TD> clb5 promoter (during G1 phase)</TD><TD >Kequan Lin</TD><TD ALIGN='right'>648</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051304'>BBa_K1051304</A></TD><TD>Regulatory</TD><TD> cln3  promoter (M phase to G1 phase)</TD><TD >Kequan Lin</TD><TD ALIGN='right'>1075</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051305'>BBa_K1051305</A></TD><TD>Regulatory</TD><TD>met16 promoter (M phase to G1 phase)</TD><TD >Zhu Shuang</TD><TD ALIGN='right'>551</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051306'>BBa_K1051306</A></TD><TD>Regulatory</TD><TD>met28 promoter (S phase)</TD><TD >Zhu Shuang</TD><TD ALIGN='right'>500</TD>
 +
</table>
 +
<h5><i>E. coli</i></h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051400'>BBa_K1051400</A></TD><TD>Regulatory</TD><TD>promoter->dnaAp1</TD><TD >Bingwei Zheng</TD><TD ALIGN='right'>359</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051401'>BBa_K1051401</A></TD><TD>Regulatory</TD><TD>promoter->dnaAp1 no RBS</TD><TD >Bingwei Zheng</TD><TD ALIGN='right'>184</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051402'>BBa_K1051402</A></TD><TD>Regulatory</TD><TD>promoter->gyrB</TD><TD >Bingwei Zheng</TD><TD ALIGN='right'>188</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051403'>BBa_K1051403</A></TD><TD>Regulatory</TD><TD>promoter->gyrB no RBS</TD><TD >Bingwei Zheng</TD><TD ALIGN='right'>158</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051404'>BBa_K1051404</A></TD><TD>Regulatory</TD><TD>promoter->ftsQp</TD><TD >Bingwei Zheng</TD><TD ALIGN='right'>485</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051405'>BBa_K1051405</A></TD><TD>Regulatory</TD><TD>promoter->ftsQp no RBS</TD><TD >Bingwei Zheng</TD><TD ALIGN='right'>198</TD>
 +
</table>
 +
<h4>Degradation Tags</h4>
 +
<h5>Yeast</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051200'>BBa_K1051200</A></TD><TD>DNA</TD><TD>ubiquitin</TD><TD >wanling ZHOU</TD><TD ALIGN='right'>1146</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051201'>BBa_K1051201</A></TD><TD>DNA</TD><TD>D-BOX</TD><TD >wanling ZHOU</TD><TD ALIGN='right'>39</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051202'>BBa_K1051202</A></TD><TD>DNA</TD><TD>cln1-PEST</TD><TD >wanling ZHOU</TD><TD ALIGN='right'>489</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051203'>BBa_K1051203</A></TD><TD>DNA</TD><TD>Cln2-PEST</TD><TD >wanling ZHOU</TD><TD ALIGN='right'>537</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051204'>BBa_K1051204</A></TD><TD>DNA</TD><TD>cln3-PEST</TD><TD >wanling ZHOU</TD><TD ALIGN='right'>588</TD>
 +
</table>
 +
<h5><i>E. coli</i></h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051206'>BBa_K1051206</A></TD><TD>Tag</TD><TD>The degradation tag in E. coil ,M0050 with TAATAA.</TD><TD >Rui Guan</TD><TD ALIGN='right'>39</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051207'>BBa_K1051207</A></TD><TD>Tag</TD><TD>The degradation tag in E. coil ,M0051 with TAATAA.</TD><TD >Rui Guan</TD><TD ALIGN='right'>45</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051208'>BBa_K1051208</A></TD><TD>Tag</TD><TD>The degradation tag in E. coil ,M0052 with TAATAA.</TD><TD >Rui Guan</TD><TD ALIGN='right'>39</TD>
 +
</table>
 +
<h4>Targeting Peptides</h4>
 +
<h5>Mitochondria</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051100'>BBa_K1051100</A></TD><TD>Coding</TD><TD>targeting peptide to mitochondria inner membrane</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>209</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051101'>BBa_K1051101</A></TD><TD>Coding</TD><TD>Cox4 targeting peptide to mitochondria inner membrane</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>75</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051102'>BBa_K1051102</A></TD><TD>Coding</TD><TD>targeting peptide to mitochondria inner membrane</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>141</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051103'>BBa_K1051103</A></TD><TD>Coding</TD><TD>TOM22, targeting peptide to mitochondria outer membrane</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>69</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051104'>BBa_K1051104</A></TD><TD>Coding</TD><TD>VDAC1;targeting peptide to mitochondria outer membrane</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>93</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051105'>BBa_K1051105</A></TD><TD>Coding</TD><TD>TOM40;targeting peptide to mitochondria outer membrane</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>147</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051106'>BBa_K1051106</A></TD><TD>Coding</TD><TD>targeting peptide to mitochondria outer membrane</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>96</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051107'>BBa_K1051107</A></TD><TD>Coding</TD><TD>Tom70;targeting peptide to mitochondria outer membrane</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>90</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051108'>BBa_K1051108</A></TD><TD>Coding</TD><TD>targeting peptide to mitochondria outer membrane;Tom20</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>120</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051109'>BBa_K1051109</A></TD><TD>Coding</TD><TD>targeting peptide to mitochondria matrix;MIA40</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>93</TD>
 +
<tr>
 +
</table>
 +
<h5>Centrosome</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051110'>BBa_K1051110</A></TD><TD>Coding</TD><TD>ScSfi1;targeting peptide to centrosome</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>63</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051111'>BBa_K1051111</A></TD><TD>Coding</TD><TD>ScSfi1E; targeting peptide to centrosome;</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>63</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051112'>BBa_K1051112</A></TD><TD>Coding</TD><TD>ScKar1;targeting peptide to centrosome</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>60</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051113'>BBa_K1051113</A></TD><TD>Coding</TD><TD>ScSac3;targeting peptide to centrosome</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>57</TD>
 +
</table>
 +
<h5>Nucleus</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051114'>BBa_K1051114</A></TD><TD>Coding</TD><TD>H2A2;targeting peptide to nucleus</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>408</TD>
 +
</table>
 +
<h5>Vacuolar</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051115'>BBa_K1051115</A></TD><TD>Coding</TD><TD>ZRC1;Vacuolar Membrane Targeting Protein</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>396</TD>
 +
</table>
 +
<h5>Actin</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051116'>BBa_K1051116</A></TD><TD>Coding</TD><TD>Actin Targeting Protein</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>1779</TD>
 +
</table>
 +
<h5>Plasma Membrane</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051117'>BBa_K1051117</A></TD><TD>Coding</TD><TD>Plasma Membrane Targeting Sequence</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>12</TD>
 +
</table>
 +
<h5>Peroxisomes</h5>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051118'>BBa_K1051118</A></TD><TD>Coding</TD><TD>ScPEX21p&#65307;baking yeast targeting peptide to peroxisome ;</TD><TD >Jinchun Zhang</TD><TD ALIGN='right'>30</TD>
 +
</table>
 +
 +
<h4>CRISPRi Verification</h4>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051800'>BBa_K1051800</A></TD><TD>Coding</TD><TD>protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.</TD><TD >Shihong Chen</TD><TD ALIGN='right'>4164</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051801'>BBa_K1051801</A></TD><TD>Device</TD><TD>Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib</TD><TD >Shihong Chen</TD><TD ALIGN='right'>388</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051801'>BBa_K1051801</A></TD><TD>Device</TD><TD>Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib</TD><TD >Shihong Chen</TD><TD ALIGN='right'>388</TD>
 +
</table>
 +
<h4>Alternative Splicing Verification</h4>
 +
<table>
 +
<TH>Name</TH><TH>Type<TH>Description<TH>Designer<TH>Length<TH>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051700'>BBa_K1051700</A></TD><TD>DNA</TD><TD>SRC1 Intron -4bp at 5'</TD><TD >Yang Zhou</TD><TD ALIGN='right'>129</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051701'>BBa_K1051701</A></TD><TD>DNA</TD><TD>SRC1 Intron+GG</TD><TD >Yang Zhou</TD><TD ALIGN='right'>132</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051702'>BBa_K1051702</A></TD><TD>DNA</TD><TD>SRC1 Intron+CGG</TD><TD >Yang Zhou</TD><TD ALIGN='right'>133</TD>
 +
<tr>
 +
<TD><A href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051703'>BBa_K1051703</A></TD><TD>DNA</TD><TD>SRC1 Intron with additional 6bp at both directions</TD><TD >Yang Zhou</TD><TD ALIGN='right'>142</TD>
 +
</table>
     </div>
     </div>
Line 135: Line 438:
<div class="media">
<div class="media">
<ul id="myGallery4">
<ul id="myGallery4">
-
           <li><img src="https://static.igem.org/mediawiki/2012/b/b5/Yao.result.jpg"/>
+
           <li><img src="https://static.igem.org/mediawiki/2013/8/8d/Sequence_Result.png" title="Sequencing Result" description="An example for part sequencing." ></li>
 +
<li><img src="https://static.igem.org/mediawiki/2013/7/74/Alignment.png" title="Alignment Result" description="An example for part sequencing result alignment." ></li>
</ul></div>
</ul></div>
     <div id="box4" class="box">
     <div id="box4" class="box">
-
         <h3>Parts Sequencing</h3><br/>
+
         <h3>Parts Sequencing</h3>
 +
<p>All of our parts have been sequenced to get verification.
 +
</p>
 +
<p>Sequencing example of K1051701
 +
</p>
 +
<img src="https://static.igem.org/mediawiki/2013/8/8d/Sequence_Result.png">
 +
<center><p>Figure. Sequencing Result</p></center>
 +
<p>Alignment example of K1051701</p>
 +
<img src="https://static.igem.org/mediawiki/2013/7/74/Alignment.png">
 +
<center><p>Figure. Alignment Result</p></center>
     </div>
     </div>

Latest revision as of 10:57, 28 October 2013


Ball Ball

Playing with my eyes
aren't you?

Hi I am Dr. Mage!
A "budding" yeast cell!

Best BioBricks

Best BioBrick (Natural/Collection), BBa_K1051303, BBa_K1051300 ~ BBa_K1051306

K1051303

K1051300

K1051301

K1051302

K1051304

K1051305

K1051306

Best BioBrick (Engineered), BBa_K1051900

K1051900

Best Measurement, BBa_K1051257 ~ BBa_K1051259

K1051257

K1051258

K1051259

Devices List

Final Constructions

Final Constructions of E.coli

K1051910

K1051911

K1051912

Final Constructions of Budding Yeast

K1051900a

K1051900b

K1051901

K1051902

K1051903

Promoter Verification

Verification of E.coli

K1051245

K1051246

K1051247

Verification of Budding Yeast

K1051307

K1051355

K1051356

K1051357

K1051358

K1051359

K1051365

K1051366

K1051367

K1051368

K1051369

Degradation Rate Measurement

Measurement of E.coli

K1051257

K1051258

K1051259

Measurement of Budding Yeast

K1051278

K1051279

K1051280

K1051281

K1051288

Targeting Peptides Verification

K1051150

K1051151

K1051152

K1051153

CRISPRi Verification

K1051852

Alternative Splicing Verification

K1051706

Basic Parts

Universal Parts

Reporters
NameTypeDescriptionDesignerLength
BBa_K1051000ReporterStop codon free RFP in RFC[23] standardXiang LI675
BBa_K1051001ReporterStop codon free ECFP in RFC[23] standardXiang LI716
BBa_K1051002ReporterStop codon free EYFP in RFC[23] standardXiang LI717
BBa_K1051003ReporterStop codon free GFP in RFC[23] standardXiang LI714
BBa_K1051004ReporterStop codon free mOrange in RFC[23] standardXiang LI738
Yeast Terminators
NameTypeDescriptionDesignerLength
BBa_K1051006TerminatorSaccharomycescerevisiae TBY-1 terminator with stopcodonXiang LI31
BBa_K1051007TerminatorS. cerevisiae SSV7 terminator with stopcodonXiang LI39
BBa_K1051008TerminatorS. cerevisiae CAA terminator with stopcodonXiang LI18
E. coli Terminators
NameTypeDescriptionDesignerLength
BBa_K1051009Terminatore.coli terminator ilvGEDA_T with stop codonXiang LI92

Cyclin Promoters

Yeast
NameTypeDescriptionDesignerLength
BBa_K1051300Regulatory cln2 promoter (G1 phase to S phase)Kequan Lin1000
BBa_K1051301Regulatory clb2 promoter (during G2 phase)Kequan Lin1063
BBa_K1051302Regulatory clb5 promoter (during G1 phase)Kequan Lin648
BBa_K1051304Regulatory cln3 promoter (M phase to G1 phase)Kequan Lin1075
BBa_K1051305Regulatorymet16 promoter (M phase to G1 phase)Zhu Shuang551
BBa_K1051306Regulatorymet28 promoter (S phase)Zhu Shuang500
E. coli
NameTypeDescriptionDesignerLength
BBa_K1051400Regulatorypromoter->dnaAp1Bingwei Zheng359
BBa_K1051401Regulatorypromoter->dnaAp1 no RBSBingwei Zheng184
BBa_K1051402Regulatorypromoter->gyrBBingwei Zheng188
BBa_K1051403Regulatorypromoter->gyrB no RBSBingwei Zheng158
BBa_K1051404Regulatorypromoter->ftsQpBingwei Zheng485
BBa_K1051405Regulatorypromoter->ftsQp no RBSBingwei Zheng198

Degradation Tags

Yeast
NameTypeDescriptionDesignerLength
BBa_K1051200DNAubiquitinwanling ZHOU1146
BBa_K1051201DNAD-BOXwanling ZHOU39
BBa_K1051202DNAcln1-PESTwanling ZHOU489
BBa_K1051203DNACln2-PESTwanling ZHOU537
BBa_K1051204DNAcln3-PESTwanling ZHOU588
E. coli
NameTypeDescriptionDesignerLength
BBa_K1051206TagThe degradation tag in E. coil ,M0050 with TAATAA.Rui Guan39
BBa_K1051207TagThe degradation tag in E. coil ,M0051 with TAATAA.Rui Guan45
BBa_K1051208TagThe degradation tag in E. coil ,M0052 with TAATAA.Rui Guan39

Targeting Peptides

Mitochondria
NameTypeDescriptionDesignerLength
BBa_K1051100Codingtargeting peptide to mitochondria inner membraneJinchun Zhang209
BBa_K1051101CodingCox4 targeting peptide to mitochondria inner membraneJinchun Zhang75
BBa_K1051102Codingtargeting peptide to mitochondria inner membraneJinchun Zhang141
BBa_K1051103CodingTOM22, targeting peptide to mitochondria outer membraneJinchun Zhang69
BBa_K1051104CodingVDAC1;targeting peptide to mitochondria outer membraneJinchun Zhang93
BBa_K1051105CodingTOM40;targeting peptide to mitochondria outer membraneJinchun Zhang147
BBa_K1051106Codingtargeting peptide to mitochondria outer membraneJinchun Zhang96
BBa_K1051107CodingTom70;targeting peptide to mitochondria outer membraneJinchun Zhang90
BBa_K1051108Codingtargeting peptide to mitochondria outer membrane;Tom20Jinchun Zhang120
BBa_K1051109Codingtargeting peptide to mitochondria matrix;MIA40Jinchun Zhang93
Centrosome
NameTypeDescriptionDesignerLength
BBa_K1051110CodingScSfi1;targeting peptide to centrosomeJinchun Zhang63
BBa_K1051111CodingScSfi1E; targeting peptide to centrosome;Jinchun Zhang63
BBa_K1051112CodingScKar1;targeting peptide to centrosomeJinchun Zhang60
BBa_K1051113CodingScSac3;targeting peptide to centrosomeJinchun Zhang57
Nucleus
NameTypeDescriptionDesignerLength
BBa_K1051114CodingH2A2;targeting peptide to nucleusJinchun Zhang408
Vacuolar
NameTypeDescriptionDesignerLength
BBa_K1051115CodingZRC1;Vacuolar Membrane Targeting ProteinJinchun Zhang396
Actin
NameTypeDescriptionDesignerLength
BBa_K1051116CodingActin Targeting ProteinJinchun Zhang1779
Plasma Membrane
NameTypeDescriptionDesignerLength
BBa_K1051117CodingPlasma Membrane Targeting SequenceJinchun Zhang12
Peroxisomes
NameTypeDescriptionDesignerLength
BBa_K1051118CodingScPEX21p；baking yeast targeting peptide to peroxisome ;Jinchun Zhang30

CRISPRi Verification

NameTypeDescriptionDesignerLength
BBa_K1051800Codingprotein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.Shihong Chen4164
BBa_K1051801DeviceTargeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhibShihong Chen388
BBa_K1051801DeviceTargeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhibShihong Chen388

Alternative Splicing Verification

NameTypeDescriptionDesignerLength
BBa_K1051700DNASRC1 Intron -4bp at 5'Yang Zhou129
BBa_K1051701DNASRC1 Intron+GGYang Zhou132
BBa_K1051702DNASRC1 Intron+CGGYang Zhou133
BBa_K1051703DNASRC1 Intron with additional 6bp at both directionsYang Zhou142

Parts Sequencing

All of our parts have been sequenced to get verification.

Sequencing example of K1051701

Figure. Sequencing Result

Alignment example of K1051701

Figure. Alignment Result

Retrieved from "http://2013.igem.org/Team:Shenzhen_BGIC_ATCG/parts"