Team:USTC CHINA/Parts

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             <ul class="subs">
             <ul class="subs">
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
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                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></li>
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                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
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<div class="conbar2">
<div class="conbar2">
<div class="leftbar" align="left" style="margin-bottom:30px;">
<div class="leftbar" align="left" style="margin-bottom:30px;">
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<div class="part-col-1">
         <h1>TD1, Transdermal peptide</h1>
         <h1>TD1, Transdermal peptide</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074000">BBa_K1074000</a></h2>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074000">BBa_K1074000</a></h2>
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         <p>TD1 is a short synthetic peptide(ACSSSPSKHCG)identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin. Studies suggested that the peptide creates a transient opening in the skin barrier to enable macromolecular material to reach systemic circulation.</p>
+
         <p align="center">TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...</p>
</div>
</div>
  <div class="part-col-1">
  <div class="part-col-1">
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         <h2>Pgrac+RBS+SamyQ+TD1+GFP</h2>
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         <h1>Pgrac+RBS+SamyQ+TD1<br>+GFP</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074006">BBa_K1074006</a></h2>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074006">BBa_K1074006</a></h2>
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         <p>This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method.</p>
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         <p align="center">This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...</p>
</div>
</div>
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<div class="clear"></div>
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  <div class="part-col-1">
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001"><h1>BBa_K1074001</h1></a>
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         <h1>PHT43</h1>
-
         <p>PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis. It is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTGinducible)promoter by addition of the lac operator. An efficient Shine-Dalgarno (SD) sequence as well as a multiple cloning site (BamH I, Xba I, Aat II, Sma I) were also inserted. To obtain secretion of recombinant proteins, the coding region for the signal peptide of the amyQ gene encoding an α-amylase was fused to the SD sequence. </p>
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        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2>
 +
         <p align="center">PHT43 is a <i>E.coli-B.subtilis</i> shuttle vector allowing high-level expression of secreted proteins in <i>B.subtilis</i>... </p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
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         <h1>Overview</h1>
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         <h1>HBsAg</h1>
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074002">BBa_K1074002</a></h2>
 +
        <p align="center">HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
-
         <h1>Overview</h1>
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         <h1>Ag85b</h1>
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074003">BBa_K1074003</a></h2>  
 +
         <p align="center">The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin... </p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
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         <h1>Overview</h1>
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         <h1>Protective Antigen Domain 4</h1>
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         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074004">BBa_K1074004</a></h2>  
 +
         <p align="center">Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax... </p>
</div>
</div>
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<div class="clear"></div>
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<div class="part-col-2">
<div class="part-col-2">
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         <h1>Overview</h1>
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         <h1>LTB</h1>
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2>
 +
        <p align="center">This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of <i>E.coli</i>...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
-
         <h1>Overview</h1>
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         <h1>Pctc+RBS+<br>amilCP</h1>
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2>  
 +
         <p align="center">Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in <i>B.subtilis</i> activated by endogenous sigma factor B ...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
-
         <h1>Overview</h1>
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         <h1>SdpA,SdpB,<br>SpbC</h1>
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2>  
 +
         <p align="center">Killing factor of <i>B.subtilis</i> ,SpbC Induces the lysis of other <i>B.subtilis</i> cells that have not entered the sporulation pathway...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
-
         <h1>Overview</h1>
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         <h1>Pgrac+RBS+SDP</h1>
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2>  
 +
         <p align="center">Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of <i>B.subtilis</i>...</p>
</div>
</div>
<div class="clear"></div>
<div class="clear"></div>
<div class="part-col-2">
<div class="part-col-2">
-
         <h1>Overview</h1>
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         <h1>PsdpRI+RBS<br>+SDP</h1>
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
      <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2>  
 +
         <p align="center">We construct this gene circuit as our kill switch to kill the engineered <i>B.subtilis</i> for the safety purpose...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
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         <h1>Overview</h1>
+
       
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
 
 +
 
 +
         <h1>Pgrac promoter</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074012">BBa_K1074012</a></h2>  
 +
         <p align="center">Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG... </p>
</div>
</div>
 +
 +
 +
<div class="part-col-2">
<div class="part-col-2">
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         <h1>Overview</h1>
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         <h1>promoter sdpR/I</h1>
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2>  
 +
         <p align="center">This part is a sigma factor A-dependent promotor of the gene sdpR derived from the <i>B.subtilis</i>...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
-
         <h1>Overview</h1>
+
 
-
         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
+
 
 +
         <h1>SamyQ</h1>
 +
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074014">BBa_K1074014</a></h2>
 +
        <p align="center">SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in <i>B.subtilis</i> WB800N...</p>
</div>
</div>
 +
 +
 +
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<div class="part-col-2">
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        <h1>RBS for Pgrac</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2>
 +
        <p align="center">An efficient RBS for promoter Pgrac(BBa_K1074012) in <i>B.subtilis</i>.../p>
 +
</div>
 +
 +
<div class="part-col-2">
 +
        <h1>RBS for Pctc</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2>
 +
        <p align="center">This is an efficient RBS for promoter Pctc(BBa_K143010) in <i>B.subtilis</i>...</p>
 +
</div>
 +
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<div class="clear"></div>
</div>
</div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
-
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background">Background</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">Design</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">Furthur Work</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/AdvancingWork">Advancing Work</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/FutureWork">Future Work</a></div>
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
</div></div></div>         
</div></div></div>         

Latest revision as of 12:33, 28 October 2013

TD1, Transdermal peptide

BBa_K1074000

TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...

Pgrac+RBS+SamyQ+TD1
+GFP

BBa_K1074006

This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...

PHT43

BBa_K1074001

PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis...

HBsAg

BBa_K1074002

HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...

Ag85b

BBa_K1074003

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin...

Protective Antigen Domain 4

BBa_K1074004

Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax...

LTB

BBa_K1074005

This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli...

Pctc+RBS+
amilCP

BBa_K1074007

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B.subtilis activated by endogenous sigma factor B ...

SdpA,SdpB,
SpbC

BBa_K1074009

Killing factor of B.subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway...

Pgrac+RBS+SDP

BBa_K1074010

Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of B.subtilis...

PsdpRI+RBS
+SDP

BBa_K1074011

We construct this gene circuit as our kill switch to kill the engineered B.subtilis for the safety purpose...

Pgrac promoter

BBa_K1074012

Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG...

promoter sdpR/I

BBa_K1074013

This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis...

SamyQ

BBa_K1074014

SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in B.subtilis WB800N...

RBS for Pgrac

BBa_K1074015

An efficient RBS for promoter Pgrac(BBa_K1074012) in B.subtilis.../p>

RBS for Pctc

BBa_K1074016

This is an efficient RBS for promoter Pctc(BBa_K143010) in B.subtilis...

Project

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