Team:BIT/project hardware

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<li><a href="https://2013.igem.org/Team:BIT/project_AC">AMPLIFIER</a></li>
<li><a href="https://2013.igem.org/Team:BIT/project_AC">AMPLIFIER</a></li>
<li><a href="https://2013.igem.org/Team:BIT/project_microchip">MICRO CHIP</a></li>
<li><a href="https://2013.igem.org/Team:BIT/project_microchip">MICRO CHIP</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_hardware">HARDWARE</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_hardware">MEASUREMENT</a></li>
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      <td width="608" class="t1">Hardware</td>
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      <td width="814" class="t1"><p class="aaa"><span class="aaa"><span class="aaa">BIT's </span></span><span class="high">measurement</span></p>
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          <p class="high"><span class="aaa"><span class="aaa"><span class="aaa">Based on</span> </span></span><a href="#sbb" class="high">Standard Biomedical Bricks</a></p>
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          <p class="high">&nbsp;</p></td>
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      <td class="t2"><strong>Introduction</strong></td>
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      <td class="t2"><p><span class="high">Measurement</span>:</p>
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        <p>&nbsp; &nbsp; &nbsp;Ok, now we have contributed a new sensor,  put forward a concept of <a href="https://2013.igem.org/Team:BIT/project_AC">controlled amplifier</a> and are working on optimizing it. But we think this is just a star, for we can then use this kind of idea to detect nearly all kinds of antibiotics in the future and we are still trying our best to do so. </p>
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          <p>&nbsp; &nbsp; &nbsp;But a matter is that we cannot see the florescence in normal condition, just like the picture below, we need UV exiting light, narrowband filter and light sensor to tell us the specific intensity of the light, we also need to calculate the material we want to detect using our mathematical model. That’s so complicated.</p></td>
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            <td class="t3"><img src="https://static.igem.org/mediawiki/2013/2/2b/BIT_H1.jpg" alt="" width="355" height="250">
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        <p class="t2">&nbsp; &nbsp; &nbsp;Here, a small, cheap and handheld device, together with our <a href="https://2013.igem.org/Team:BIT/project_microchip">micro chip</a> , is produced which together with our biological core, forms a complete detecting system. If you are interested in the detailed structure of our detecting system, please click <a href="http://v.youku.com/v_show/id_XNjI3NDk1ODAw.html">Here</a>.</p>
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            <td class="t3"><img src="https://static.igem.org/mediawiki/2013/0/06/BIT_H2.png" alt="" width="736" height="299">
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            <td width="739"><img src="https://static.igem.org/mediawiki/2013/d/d1/BITsam.jpg" alt="" width="233" height="192"><img src="https://static.igem.org/mediawiki/2013/8/85/BITchi.jpg" alt="" width="233"><img src="https://static.igem.org/mediawiki/2013/c/c7/BITdet.jpg" alt="" width="233" height="195"></td>
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            <td><p class="t2"><img src="https://static.igem.org/mediawiki/2013/c/cd/BITsys.jpg" alt="" width="700"></p></td>
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      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;As the joint of biological and non-biological research, this device aims to detect the fluorescent intensity of GFP and calculate the concentration of the chemicals (Cr (VI), beta-lactam, and tetracycline, respectively) detected based on it. We can then assess the quality of the sample detected.
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        <p class="t2"><span class="aaa"><span class="high"><span class="t2">Parameters of the system :</span></span></span></p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Cost: 260RMB/$40 (device &amp; chip). </p>
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            <td class="t3"><img src="https://static.igem.org/mediawiki/2013/0/00/BIT_H3.png" alt="" width="450" height="350">
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Size: 10cmX6.5cmX5cm</p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Power: 20W Max, 3W Average</p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Functions: User-define mathematical model. Fast detection. Temperature &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;  &nbsp;&nbsp;Control . </p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Resolution: 5 ng/ml Standard fluorescein sodium, 2~5ng/ml Tet.</p>
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    <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp; The mechanism of this device is simple (figure). The exciting light coming from blue LED through 490nm narrowband filter, which only allow 490nm light  to pass through. The filtered light then penetrates our test chip. If GFP exists on our test chip, it would transform the frequency of the excitation light into about 520nm, which is the only frequency of light that can pass through the 517nm narrowband filter. Then our sensor will be able to detect the intensity of the light and calculate the content of GFP, indicating the composition of the tested sample with our mathematical model.<br>
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<p class="t2">&nbsp; &nbsp; &nbsp;The small device uses normal color sensors and light path similar to UV spectrophotometer, but contains a new, or rather strange mathematical model, a combination of our biological model and relative non-biological equations. </p>
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          &nbsp;&nbsp;&nbsp;&nbsp; The characteristic of our device is that it is really CHEAP. Although it costs less than 300RMB in total and can easily cooperate with our biological products.
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        <p class="t2">&nbsp; &nbsp; &nbsp;For the specific data of the measure system, please click <a href="https://2013.igem.org/Team:BIT/project_data">Here.</a>         </p>
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        <p class="t2">&nbsp;</p>
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        <p class="t2"><span class="high">Standard Biomedical Bricks</span><a name="sbb"></a></p>
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      <td class="t2"><strong>Result</br></br>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Well, as for biological detection of harmful materials, such as <span class="aaas"><span class="ww">antibiotics, heavy metals, aromatic hydrocarbons, radioactive substances, pesticide residues</span></span> and so on, we need a biological output in <span class="ww">color, fluorescence, conductivity, OD value </span>and many other forms.</p>
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A) Linear Regression</strong></td>
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        <p class="t2"><img src="https://static.igem.org/mediawiki/2013/4/43/BITaa.gif" width="700" height="500"></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;But those outputs may be so weak, just like our fluorescence, without the <a href="https://2013.igem.org/Team:BIT/project_AC" class="ww">controlled amplifier</a>, it can hardly be seen by our eyes, even under UV light.</p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Biomedical machines are used in this case, but  those machines can hardly be found out of the lab.</p>
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    <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp; To indicate the reliability of our system, we conducted experiments with fluorescein sodium solutions of different concentration. The figure below shows the curve of our result, which fits perfectly with the linear curve with an R-squared of 0.9842. The result indicates that our detection system is reliable.</td>
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        <p class="t2">&nbsp; &nbsp; &nbsp; So we put forward <span class="high"><span class="ww">standard biomedical &amp; biological system</span></span>, that is: to design a biological core, finishing the corresponding mathematical model, and a standard biomedical external device. </p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Just like us, our aim is <span class="ww"><span class="aaa"><span class="ww">Practical Application</span></span></span>. We created a biosensor, an amplifier and their mathematical models. </p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;<span class="ww">Standard biomedical bricks</span> means the specific mathematical models which suits for our low cost sensors. iGEM is an application-oriented contest, so detection needs to be economic and convenient. Now we have many standard electronic sensors, BIT iGEM team has also optimized a processor which has an interface suiting for any kinds of sensors.<span class="ww"> What we need to do</span> is to <span class="ww"><span class="t2"><span class="aaa"><span class="ww">build up our engineered bacteria</span></span></span></span>,<span class="ww"> burn the standard mathematical models in the processor</span> and <span class="ww">connect the suitable sensor to the processor</span>. Then a complete and application-oriented system is built. Just like our detecting system, we have made many editions :</p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Then we arrange user-friendly I/O interface to the standard processor, making a colorful cover to the whole system. That means, you can achieve any kinds of detection using the same device, that sounds cool, isn’t it?</p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;So what we have done at this time is just to simplify the old measurement and provide a new, convenient, economic measurement which detection can be achieved in kitchen. Maybe, some years later, you can see a &quot;bio-antibiotic detector&quot; in supermarket, and let's wait for the coming of that day !</p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;We have put forward a concept, and now we have make part of the concept into reality. We will go further on our way to overcome any difficulties, and we eagerly need partners to fight with us. If you have interest in it, please <a href="mailto:hqing@bit.edu.cn;824074989@qq.com">CLICK HERE</a> to contact us.</p>
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    <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp; We conducted a “Blind test”, in which we detected the concentration of antibiotics in pure water. In the test, we had seven samples:</br>
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1) Five SAFE samples, with only pure water;</br>
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2) One HARMFUL sample, with a low concentration of antibiotics;</br>
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<p class="t2">&nbsp;</p>
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3) One TOXIC sample, with a high concentration of antibiotics.</br>
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Before the test, we did not know which sample was safe and which sample was harmful. Both of the conventional method (VarioskaFlash) and our new method were adopted to detect the concentration of antibiotics in the sample and here is the result. As can be seen from the figure below, the data of our detector was consistent with that of VarioskaFlash, which means our system really worked well.
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         <td> E-mail: yifei0114@bit.edu.cn</td>
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         <td> E-mail:<a href="mailto:yifei0114@bit.edu.cn"> yifei0114@bit.edu.cn</a></td>
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Latest revision as of 14:14, 28 October 2013

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